Multicolor immunofluorescence analyses were performed using 3-μm-thick sections of formalin-fixed paraffin-embedded (FFPE) tissues. Briefly, slides were deparaffinized in xylene and hydrated in a series of decreasing graded ethanol series. After heat-induced antigen retrieval in citrate buffer (pH = 6), samples were permeabilized with 0.5% Triton X-100, blocked with 5% goat serum-phosphate-buffered saline (PBS), and sequentially co-stained with antibodies recognizing VCAM-1 (Abcam, ab134047), CD248 (Abcam, ab217535), 4-HNE (Abcam, ab46545), FAPα (Invitrogen, BMS168; Abcam, ab218164), Mfap4 (Thermo, PA5-24865), Sparcl1 (Santa Cruz, sc-514275), F4/80 (Cell Signaling Technology, #70076), rabbit IgG Isotype Control (Invitrogen, 31235), mouse IgG Isotype Control (Invitrogen, 14-4714-82), and DAPI. A TSA indirect kit (PerkinElmer) was used according to the manufacturer’s instructions. Vectra® Polaris™ Imaging System (Akoya Biosciences) was used to collect images, and image analysis was performed using HALO™ Image Analysis Software.
Ab46545
Manufactured by Thermo Fisher Scientific
Sourced in United States
Ab46545 is a laboratory instrument designed for high-precision measurements. It features advanced technology to ensure accurate and reliable data collection. The core function of this product is to provide researchers with a versatile and dependable tool for their laboratory work.
Automatically generated - may contain errors
Lab products found in correlation
F4 80, by Cell Signaling Technology (1 mentions)
Tsa indirect kit, by PerkinElmer (1 mentions)
Vectra polaris imaging system, by Akoya Biosciences (1 mentions)
Ab134047, by Abcam (1 mentions)
Rabbit igg isotype control, by Thermo Fisher Scientific (1 mentions)
Mouse igg isotype control, by Thermo Fisher Scientific (1 mentions)
2 protocols using ab46545
1
Multicolor Immunofluorescence of FFPE Tissues
2
Methylarsonic Acid Metabolism Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We purchased all chemicals from Sigma-Aldrich (St Louis, MO, USA) if not contrarily indicated, 99% pure methylarsonic acid (MMAV) disodium salt from Chem Service (West Chester, PA, USA); sodium borohydride from EM Science (Gibbstown, NJ, USA). Santa Cruz Biotechnology (Santa Cruz, CA, USA) provided the following primary antibodies for Western blots: SIRT1 (B-7, sc74465), PGCα1 (D-5, sc-518025), NRF1 (147.1, sc-101102), TFAM (F-6, sc-166965), MT-CO2 (D-5, sc-514489), GFAP (GA-5, sc-58766), and secondary antibody anti-mouse IgM (sc-2064). From Proteintech (Rosemont, IL, USA) Anti-SLC25A39 (14,963-1-AP) was purchased. Cell Signaling Technology (Danvers, MA, USA) provided Anti-SLC1A4 (8442 s) antibody and secondary goat anti-rabbit antibodies. Abcam (Cambridge, MA, EE. UU) provided anti-4-Hydroxynonenal antibody (ab46545) and anti-ATF-4 antibody (ab23760), and Invitrogen (Waltham, MA, USA), the secondary anti-mouse IgG antibody.
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