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Triglycerides liquicolor test

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The Triglycerides LiquiColor Test is a quantitative diagnostic test used to measure the concentration of triglycerides in human serum or plasma samples. The test utilizes a colorimetric method to determine the triglyceride levels.

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Lab products found in correlation

Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Pierce bicinchoninic acid bca assay, by Thermo Fisher Scientific (1 mentions) Glycogen colorimetric assay kit, by Abcam (1 mentions) Porcine trehalase, by Merck Group (1 mentions) Glucose hk assay kit, by Merck Group (1 mentions) Glucose liquicolor test, by EKF Diagnostics (1 mentions) Rat mouse insulin elisa kit, by Merck Group (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions) Pierce bca assay, by Thermo Fisher Scientific (1 mentions)

4 protocols using triglycerides liquicolor test

1

Fly Triglyceride Measurement Protocol

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Total triglycerides normalized to total protein were measured as described in (14 (link)). Briefly, two flies per biological replicate were homogenized in lysis buffer [140 mM NaCl, 50 mM tris-HCl (pH 7.4), and 0.1% Triton X-100] containing protease inhibitor cocktail (Thermo Fisher Scientific). Lysate extract was used to determine protein and triglyceride concentrations using Pierce bicinchoninic acid (BCA) assay (Thermo Fisher Scientific; absorbance, 562 nm) and Triglycerides LiquiColor Test (Stanbio; abs, 500 nm), respectively.
Vaziri A., Khabiri M., Genaw B.T., May C.E., Freddolino P.L, & Dus M. (2020). Persistent epigenetic reprogramming of sweet taste by diet. Science Advances, 6(46), eabc8492.
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2

Lipid and Carbohydrate Metabolism Assays in Drosophila

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For Glucose, Glycogen and TAG assays, 5 flies, in triplicate, were homogenized in PBST, heated to 70° for 10 minutes and centrifuged; supernatant was collected. Samples were processed and levels measured using manufacturer's protocols: Glucose (HK) Assay Kit (Sigma), Glycogen Colorimetric Assay Kit (BioVision) and Triglycerides LiquiColor Test (Stanbio Laboratory), respectively. Protein levels were determined with the BCA Protein Assay Kit (Thermo Fisher Scientific) and used for normalization.
For circulating trehalose assays, 1 μl of hemolymph, in triplicate, was collected by centrifugation and diluted in Trehalase Buffer. Samples were heated at 70° for 10 minutes and treated with porcine trehalase (Sigma). Levels were measured using the Glucose (HK) Assay Kit (Sigma) following manufacturer's protocol. Total levels were calculated after subtracting free glucose and normalized per fly.
Figueroa-Clarevega A, & Bilder D. (2015). Malignant Drosophila tumors interrupt insulin signaling to induce cachexia-like wasting. Developmental cell, 33(1), 47-55.
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3

Metabolic Biomarker Measurements

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Serum glucose (Glucose Liquicolor test, Stanbio Laboratory, Boerne, Texas, USA), insulin (Rat/mouse insulin ELISA kit, Millipore Sigma, Burlington, Ontario, Canada) and triglycerides (Triglycerides Liquicolor test, Stanbio Laboratory, Boerne, Texas, US) were measured using commercially available ELISA-based kits following the manufactures instructions. Homeostatic Model Assessment of Insulin Resistance (HOMA) was calculated based on the standard formula (HOMA-IR = (fasting serum insulin (μU/mL)*fasting serum glucose (mmol/L)/22.5).
Mayengbam S., Chleilat F, & Reimer R.A. (2020). Dietary Vitamin B6 Deficiency Impairs Gut Microbiota and Host and Microbial Metabolites in Rats. Biomedicines, 8(11), 469.
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4

Fly Triglyceride and Protein Quantification

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Total triglycerides normalized to total protein were measured as described in (72) . Briefly, two flies per biological replicate were homogenized in lysis buffer (140 mM NaCl, 50 mM Tris-HCl pH 7.4, 0.1% Triton-X) containing protease inhibitor (Thermo Scientific). Lysate extract was used to determine protein and triglyceride concentrations using Pierce BCA assay (Thermo Scientific, abs 562 nm) and Triglycerides LiquiColor test (Stanbio, abs 500 nm), respectively.
Vaziri A., Khabiri M., Genaw B.T., May C.E., Freddolino P.L., & Dus M. (2020). Persistent Epigenetic Reprogramming of Sweet Taste by Diet.
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