AA-5HT, 2-AG, AM-251, AM-630, OEA, PEA and URB597 were obtained from Biomol GmbH (Hamburg, Germany). AEA was purchased from Enzo Life Sciences GmbH (Lörrach, Germany). Dimethyl sulfoxide (DMSO), ethylenediaminetetraacetic acid (EDTA), glycerol, glycine, hydrogen peroxide (H2O2), sodium chloride (NaCl), Tris hydrocloride (Tris-HCl) and Tris ultrapure were obtained from AppliChem (Darmstadt, Germany). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was bought from Ferak (Berlin, Germany). Aprotinine, bovine serum albumin, capsazepine, HCl, leupeptine, luminal, orthovanadate, para-coumaric acid, para-formaldehyde, phenylmethylsulfonyl fluoride (PMSF) and Triton® X-100 were from Sigma (Taufkirchen, Germany). Dulbecco's modified eagle medium (DMEM) with 4.5 g/l glucose and with L-glutamine was provided by Lonza (Cologne, Germany). Fetal calf serum (FCS) and penicillin-streptomycin were purchased from Invitrogen (Darmstadt, Germany) and phosphate-buffered saline (PBS) was provided by PAN Biotech (Aidenbach, Germany). Milk powder was obtained from Bio-Rad Laboratories GmbH (Munich, Germany). Acrylamide (Rotiphorese® Gel 30) was obtained from Carl Roth GmbH (Karlsruhe, Germany). Pure standards for LC-MS analysis of AEA, 2-AG, OEA, PEA and AEA-d8 were purchased from Cayman Chemical (Ann Arbor, MI, USA).
Luminal
Manufactured by Merck Group
Sourced in United States, Germany
Luminal is a laboratory equipment product manufactured by Merck Group. It is designed to provide a controlled and consistent environment for various scientific experiments and analyses. The core function of Luminal is to maintain precise temperature, humidity, and other environmental conditions as required for the specific application.
Automatically generated - may contain errors
Lab products found in correlation
Glycerol, by AppliChem (2 mentions)
Phosphate buffered saline (pbs), by PAN Biotech (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
P coumaric acid, by Merck Group (2 mentions)
Aprotinine, by Merck Group (1 mentions)
Leupeptine, by Merck Group (1 mentions)
Orthovanadate, by Merck Group (1 mentions)
Milk powder, by Bio-Rad (1 mentions)
Phenylmethylsulfonyl fluoride pmsf, by Merck Group (1 mentions)
Tris ultrapure, by AppliChem (1 mentions)
Para coumaric acid, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Rotiphorese gel 30, by Carl Roth (1 mentions)
Aea d8, by Cayman Chemical (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Capsazepine, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by AppliChem (1 mentions)
6 protocols using luminal
1
Cannabinoid Signaling Pathway Compound Acquisition
2
Reagent Procurement for Cell Culture Assays
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NS-398 was purchased from Alexis Deutschland GmbH (Grünberg, Germany). Aprotinin, Δ-glycerophosphate, ethylenediaminetetraacetic acid (EDTA), leupeptin, lovastatin lactone, luminal, mevastatin, p-coumaric acid, phenylmethylsulfonyl fluoride (PMSF), (R)-mevalonic acid lithium salt, sodium molybdate and sodium orthovanadate were obtained from Sigma-Aldrich (Taufkirchen, Germany). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was from Ferak (Berlin, Germany). Dimethyl sulfoxide (DMSO), dithiothreitol (DTT), glycerol, p-nitrophenylphosphate, sodium chloride, sodium dodecylsulfate (SDS) and sodium fluoride were from AppliChem (Darmstadt, Germany) and GW9662 and Nonidet® P-40 from Enzo Life Sciences (Lörrach, Germany). Lovastatin hydroxy acid, sodium salt, was provided from Toronto Research Chemical (Toronto, Canada) and Triton® X-100, acetonitrile (LC-MS grade) and trifluoroacetic acid (analytical grade) from Roth (Karlsruhe, Germany). Penicillin-streptomycin was from Invitrogen (Darmstadt, Germany). Dulbecco's Modified Eagle's medium (DMEM) with 4 mM L-glutamine and 4.5 g/L glucose was from Lonza (Cologne, Germany). Phosphate-buffered saline (PBS) and fetal calf serum (FCS) were obtained from PAN Biotech (Aidenbach, Germany).
3
Chemiluminescence Assay for ROS Quantification
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The ROS levels in culture media were evaluated by chemiluminescence assay (26 (link)) using 5-amino-2,3-dihydro-1,4- phthalazinedione (luminal; Sigma, St. Louis, MO, USA) as a probe in a luminometer (Synergy H1 Hybrid Reader; Biotek, USA). Each sample was scanned at 37°C for 15 minutes by the luminometer. ROS values were expressed as relative light units (RLUs). All the samples were measured in duplicate.
4
Measuring Chitin-Induced ROS Burst in Rice
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The method for measuring the chitin-induced ROS burst levels is described in [54 (link)]. Briefly, leaf discs were cut from 7-day-old rice seedlings and floated on water overnight, and then the water was removed. After that, the leaf discs were incubated with 100 µL reaction solution (20 µM luminal, Sigma Aldrich, Saint Louis, MO, USA, Cat. No. 123072 and 2.5 µg/mL peroxidase, Solarbio Science & Technology Co., Ltd. Beijing, China, Cat. No. P8020) containing 400 nM chitin (Sigma-Aldrich, Saint Louis, MO, USA, Cat. No. H1396) to immediately detect the ROS burst. The luminescence was measured by a Berthold Mithras luminometer (Berthold Technologies GmbH & Co.KG, Bad Wildbad, Germany) every 1~2 min for 55 min. Eight replicates were performed for each sample.
5
Luminescent Measurement of H2O2 in Tomato Leaves
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The peptides AtPep1 and flg22 with purity of over 95% were synthesized by China Peptides Company Limited. Tomato leaf disks of 3 mm at diameter were dipped in 200 μl of distilled water in a 96-well plate in dark over night. Water was replaced with 200 μl solution containing 100 μM luminal (Sigma-Aldrich) and 1 μg of horseradish peroxidase. AtPep1 (10 nM) and flg22 (100 nM) were added and immediately H2O2 were measured for 30 min as luminescence using a luminometer (Glomax 96 microplate luminometer, Promega).
6
Assessment of Kidney Injury Markers
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PKR specific inhibitor C16 (imoxin), Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME hydrochloride), poly-L-lysine solution (0.1 % w/v in H2O), Bicinchonic acid assay kit (BCA-1), 4′,6-diamidino-2-phenylindole (DAPI), RIPA buffer, Protease inhibitor, Phenyl methyl sulfonyl fluoride Eosin, hematoxylin, luminal, and p-Coumaric acid were purchased from Sigma Aldrich (St. Louis, MO, USA). TUNEL assay kit and Masson's trichrome kit were purchased from Abcam (Milton, Cambridge, UK). Commercial kits for assessment of serum creatinine, blood urea nitrogen, (BUN) were purchased from Arkray Health care Pvt. Ltd, Surat, Gujrat, India. Dpx mountant media (Ref:Lamb/Dpx).
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