For gut morphological studies, adult C. longicaudata and T. domestica were anesthetized for 10 min at 4°C and dissected under a Zeiss Stemi 2000-C stereo microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY). The gut was carefully dissected from the rest of the body and images were taken with a Canon DS126311 camera (Canon, Ota, Tokyo, Japan) mounted on the stereo microscope. Adult C. longicaudata and T. domestica for histological studies were sacrificed by incubation at -20°C for ten minutes and then fixed in Carnoy’s solution (60% ethanol, 30% chloroform, and 10% glacial acetic acid) for four hours at 4°C. After fixing, whole insects were transferred to 70% ethyl alcohol and sent to the Biomedical and Diagnostic Services, University of Tennessee College of Veterinary Medicine (Knoxville, TN) for sectioning and staining with hematoxylin and eosin. Histological sections were examined and documented using an Olympus BX63F upright microscope (Olympus Corporation, Shinjuku, Tokyo, Japan).
Stemi 2000 c stereomicroscope
Manufactured by Zeiss
Sourced in Germany, United States
The Stemi 2000-C stereomicroscope is a compact and versatile instrument designed for a wide range of applications. It offers a magnification range of 6.4x to 40x, providing users with detailed observations of their samples. The stereomicroscope features a binocular viewing system, allowing for comfortable and ergonomic use. Its LED illumination ensures consistent and reliable lighting during the examination process.
Automatically generated - may contain errors
Lab products found in correlation
Ccd camera, by Zeiss (2 mentions)
Ht90132, by Zeiss (2 mentions)
Axiocam icc3 digital camera, by Zeiss (2 mentions)
Eos 90d, by Canon (2 mentions)
Ds126311 camera, by Canon (1 mentions)
Bx63f upright microscope, by Olympus (1 mentions)
Axiocam erc5, by Zeiss (1 mentions)
Zen 2011 imaging software, by Zeiss (1 mentions)
Gryphax naos 20 megapixel full hd usb 3.0 color digital microscope camera, by Jenoptik (1 mentions)
Lr white resin, by Merck Group (1 mentions)
B 600tifl microscope, by Optika (1 mentions)
X gluc, by Duchefa Biochemie (1 mentions)
Eos 90d digital camera, by Canon (1 mentions)
Powershot g16 digital camera, by Canon (1 mentions)
Primo star light microscope, by Zeiss (1 mentions)
Zen software, by Zeiss (1 mentions)
35 mm petri dishes, by Sarstedt (1 mentions)
Mitotracker orange cmtmros, by Thermo Fisher Scientific (1 mentions)
Axiocam erc5s digital camera, by Zeiss (1 mentions)
Spectra x led lamp, by Lumencor (1 mentions)
53 protocols using stemi 2000 c stereomicroscope
1
Gut Morphology Analysis of C. longicaudata and T. domestica
2
In Vitro Wound Healing Assay
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Reference points 2 mm apart were made using an ultra-fine-tip sharpie on the underside of the culture plate prior to addition of cells. Following the recovery period, cells were either treated with various inhibitor concentrations or left untreated, depending on treatment group. Wound was made 5 days following recovery period, whereby cells were washed once with PBS and a 2 cm “wound” was made using a 1-mm-diameter ART10 pipette tip (cat:2139, ThermoScientific). Cells were then washed an additional two times with PBS to remove excess cellular debris, followed by the addition of appropriate media and treatment. Digital photographs of the wound were taken at 50× magnification using the ZEISS Stemi 2000-C Stereo Microscope mounted with AxioCam ERc5s (ZEISS) and developed using ZEN 2011 Imaging software (ZEISS). Photos were taken at 12-h intervals for 72 h. Media and inhibitor treatment was changed every 24 h prior to photographs to ensure wound clarity. Contrast and sharpness of images were corrected manually for purposes of clarity utilizing Photoshop CC (Adobe Systems).
3
Chorioretinal Dissection and Imaging
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Whole globes were opened at the limbus, anterior segments were removed, and the posterior eyecups were examined microscopically (Zeiss Stemi 2000-C Stereo Microscope; Carl Zeiss, Inc.). One eye from each donor was cryopreserved while the second eye was processed for retinal and choroidal flat-mounts. Digital images of the eyecups were captured (Gryphax NAOS 20 Megapixel Full HD USB 3.0 Color Digital Microscope Camera; Jenoptik, Rochester Hills, MI, USA) using reflected and transmitted illumination prior to further dissection. Vitreous was removed and retinas were then excised from the RPE/choroid and placed overnight in 2% paraformaldehyde (PFA) in 0.1 M cacodylate buffer at 4°C. Eye cups containing the choroid with the RPE intact were reimaged before they were immersed in 1% EDTA (disodium salt; dihydrate crystal; Baker Chemical Co., Radnor, PA) in distilled water for 2 hours at room temperature to facilitate removal of the RPE. Any adherent RPE cells were removed by pipetting the choroid with EDTA solution from a syringe with a blunted 25-gauge needle. Gross digital images of choroids were captured again without RPE. RPE-denuded choroids were then dissected from the sclera, washed briefly in 0.1 M cacodylate, and fixed overnight in 2% PFA in 0.1 M cacodylate buffer at 4°C.
4
Seed Germination and Seedling Growth Assays
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To assess germination, seeds were plated on horizontal MS plates, germination rate determined as seed coat rupture after 2 days scored under a Zeiss Stemi 2000-C stereo microscope.
For root and hypocotyl length measurements, seedlings were imaged using Zeiss Stemi 2000-C stereo microscope and measured using ImageJ software (http://rsbweb.nih.gov/ij/index.html ).
To assess etiolation, seedlings of indicated lines were sown on sucrose free MS, exposed to 100 μE m−2 S−1 fluorescent light for 4 h, followed by 3 days at the dark. Hypocotyl length quantified as described above.
For root GA response assays, seeds were germinated on MS were transferred to 5 μM paclo after 4 days. On the next day, root length was marked. 1 μl of 5 μM GA3 diluted in water was applied to root tips for the three subsequent days. Roots were imaged and measured on day 10.
For root and hypocotyl length measurements, seedlings were imaged using Zeiss Stemi 2000-C stereo microscope and measured using ImageJ software (
To assess etiolation, seedlings of indicated lines were sown on sucrose free MS, exposed to 100 μE m−2 S−1 fluorescent light for 4 h, followed by 3 days at the dark. Hypocotyl length quantified as described above.
For root GA response assays, seeds were germinated on MS were transferred to 5 μM paclo after 4 days. On the next day, root length was marked. 1 μl of 5 μM GA3 diluted in water was applied to root tips for the three subsequent days. Roots were imaged and measured on day 10.
5
Angiogenic Potential of Hydrogel Implants
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A CAM assay was performed as previously described [19 (link)]. White fertilized chicken eggs were incubated at 37 °C in a temperature incubator (Termaks KB8000, Norway) for 3 days. After this, a window was opened into the shell to evaluate embryo viability. Prevascularized spongy-like hydrogels BP (n = 10), and after preservation with HTS (n = 10) or α-MEM (n = 10) were implanted on the CAM at day 10 of embryonic development, and the eggs returned to the incubator at 37 °C. Control groups with empty materials (n = 10) and without the material (n = 10) were also set up. After 4 days of implantation, embryos were sacrificed with 4 % (v/v) paraformaldehyde and subsequent incubation at −80 °C for 10 min. Then, the implanted materials with adjacent portions of CAM were cut and fixed with 4% (v/v) paraformaldehyde. Ex ovo images were captured using a Stemi 2000-C stereo microscope (ZEISS, Germany).
6
GUS Activity Detection in Arabidopsis
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GUS activity detection was performed by immersing the plantlets in a buffer containing potassium ferro- and ferricyanide (0.5 mM each), 50 mM sodium phosphate, 10 mM EDTA, and 0.1% Triton X-100, 10% methanol, and 1 mg/mL X-GLUC (Duchefa). After overnight staining, Arabidopsis plantlets or tissue samples were fixed and embedded in LR White resin (Sigma) following a protocol from Dr. Nicholas Harris (Dept. of Biological Sciences, U. Durham, ftp://ftp.arabidopsis.org/home/tair/Protocols/compleat_guide/2_fix_and_embed.pdf , accessed on 26 July 2022). Resin pieces were sectioned at 2 µm thickness and counterstained with 2% aqueous fuchsine. Light and fluorescence microphotographs were taken using a B-600TiFL microscope (Optika, Ponteranica (BG), Italy), and whole plantlets were photographed under a Stemi 2000-C stereomicroscope (Zeiss, Oberkochen, Germany).
7
Thrashing Assay for Worm Motility
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All thrashing assays were done at room temperature (21.5–22.5°C). Thrashing assays were performed in 12‐well plates with 2 ml of M9 buffer per well. Individual worms were transferred to M9 buffer and allowed to recover for 20–30 s. Movies of 30 s were captured using IC Capture software with a DBK‐21BUC03 digital camera (ImagingSource, Germany) and a Zeiss Stemi 2000‐C Stereo Microscope. Movies were saved as AVI format and analyzed using ImageJ wrMTrck plugin (ImageJ1.48) for the number of body bends and the average speed. A body bend was defined as a change in the direction of the segment from the head to the midbody of an animal. The average speed was calculated as track length (mm)/time(s).
8
New Species Identification Protocol
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Adults were collected by insect net and light trap. Type specimens of the new species in this study were deposited in the Entomological Museum of China Agricultural University , Beijing, China (CAU ) and the Entomological Museum of Qingdao Agricultural University, Shandong, China (QAU ). Studies were based on whole-animal preparations and dissections. Photographs were captured by a Canon EOS 90D digital camera through a macro lens. Genitalia were prepared by immersing the apical portion of the abdomen in warm lactic acid for 0.5–1 hours. Specimens were examined and illustrations prepared by using a ZEISS Stemi 2000-C stereomicroscope. After examination, the removed abdomen was transferred to fresh glycerine and stored in a microvial pinned to the respective specimen. Structural terminology is based primarily on Courtney (2000b) .
9
Visualizing Rhizobium Cellulose Production
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Cellulose production by native Rhizobium strains was assessed using Yeast Extract Mannitol (YEM) medium (10 g mannitol, 0.5 g K2HPO4, 0.2 g MgSO4, 0.1 g NaCl, 3.0 g CaCO3, and 3.0 g yeast extract per 10 g, pH 6.8), supplemented with 0.25% Congo Red. Congo Red, a dye that binds to β 1–4 bonds found in cellulose-like polysaccharides, enabled the visualization of red-stained bacterial colonies. The inoculated plates were incubated at 28°C for 5 days and observed using a Zeiss® Stemi 2000-C Stereo Microscope to detect stained colonies (Robledo et al., 2012 (link)).
10
Larval Growth and Imaging
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16 L1 larvae were moved to a new 60 mm NGM plate and incubated at 26 °C for 5 days. After 5 days the plates were imaged using a PowerShot G16 Digital Camera (Canon, Tokyo, Japan) mounted on a Zeiss Stemi 2000‐C Stereo Microscope (ZEISS, Jena, Germany).
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