Antibodies employed were: anti-lamin A/C, goat polyclonal (Byorbit orb37882, Cambridge, UK) used at 1:100 dilution for IF and in situ proximity ligation assay (PLA); anti-lamin A/C (E1, Santa Cruz Biotechnology, Dallas, TX, USA) used at 1:500 dilution for IF and in situ proximity ligation assay (PLA) and 1:2000 for WB; anti-desmin (Abcam Ab15200 Cambridge, UK) used 1:1000 for IF and 1:2000 for PLA and WB; anti-desmin (Chemicon 1:400 for IF; phalloidin (Sigma-Aldrich, St. Louis, MO, USA) 1:1000 for IF; anti H3k9ac (Abcam, Cambridge, UK) 1:200 for IF; anti YAP (Santa Cruz Biotechnology, Dallas, TX, USA) 1:100 for IF; anti-emerin (Monosan, Uden, The Netherlands) 1:100 for IF; anti-FLAG tag (Sigma-Aldrich, St. Louis, MO, USA) 1:1000 for IF; anti-plectin 1 (D6A11, Cell signaling Technology, Danvers, MA, USA) 1:100 for IF and PLA.
Anti lamin a c e1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-lamin A/C (E1) is a mouse monoclonal antibody that recognizes the lamin A/C protein. Lamin A/C is a structural protein that is a component of the nuclear lamina, a scaffolding-like structure that provides mechanical support and organization to the cell nucleus. This antibody can be used to detect and localize lamin A/C in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Santa Cruz Biotechnology (2 mentions)
Anti lamin a c n 18, by Santa Cruz Biotechnology (2 mentions)
Odyssey blocker pbs, by LI COR (2 mentions)
Anti flag tag, by Merck Group (1 mentions)
Anti desmin, by Abcam (1 mentions)
Anti h3k9ac, by Abcam (1 mentions)
Anti yap, by Santa Cruz Biotechnology (1 mentions)
Ecl detection system, by Thermo Fisher Scientific (1 mentions)
Nonspecific immunoglobulins, by Santa Cruz Biotechnology (1 mentions)
Protein a g, by Santa Cruz Biotechnology (1 mentions)
Odyssey clx imaging system, by LI COR (1 mentions)
Image studio software version 5, by LI COR (1 mentions)
Rabbit anti ha c29f4, by Cell Signaling Technology (1 mentions)
Irdye 680lt goat anti mouse, by LI COR (1 mentions)
Anti arid1a psg3, by Santa Cruz Biotechnology (1 mentions)
Anti arid2 d8d8u, by Cell Signaling Technology (1 mentions)
Odyssey clx, by LI COR (1 mentions)
Irdye 800cw goat anti rabbit, by LI COR (1 mentions)
Anti pias1 d33a7, by Cell Signaling Technology (1 mentions)
Anti ha c29f4, by Cell Signaling Technology (1 mentions)
6 protocols using anti lamin a c e1
1
Antibodies Used for Lamin, Desmin, and Chromatin Analysis
2
Immunoprecipitation and Western Blot Analysis
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For immunoprecipitation analysis, stretched myoblasts were lysed in high detergent-IP buffer containing: 50 mM Tris-HCl (pH = 7.5), 150 mM NaCl, 0.1% SDS, 1% NP-40, 1 mM DTT, 1.5 mM MgCl, 20 mM NaF, 1 mM PMSF, and protease and phosphatase inhibitors. For each sample, 500 µg of lysate was incubated at 4 °C overnight with 2.5 µg of anti-lamin A/C (E1, Santa Cruz Biotechnology, Dallas, TX, USA) or nonspecific immunoglobulins form Santa Cruz as a negative control. After the addition of 30 μL of protein A/G (Santa Cruz Biotechnology, Dallas, TX, USA) for 60 min at 4 °C, the immunoprecipitated proteins were washed 3 times in IP buffer. Later, the samples were added to Laemmli’s buffer, boiled at 100 °C for 5 min, and subjected to Western blot analysis. Immunoblotted bands were detected by ECL detection system (Thermo Fisher Scientific, Waltham, MA, USA) and an intensity measurement was performed using a Bio-Rad MP Imaging System with Image Lab Touch Software version 3.0.1.14 (Bio-Rad Laboratories, Segrate, Milan, Italy).
3
Immunoblotting Antibody Dilutions
4
Western Blotting Immunodetection Procedure
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In all, 15 µg of protein was loaded into each well of an SDS-PAGE gel and transferred onto a PVDF membrane. Blots were blocked at room temperature for 1 hour in Odyssey Blocker PBS (Li-COR) + 2.5% BSA. Blots were incubated with primary antibody overnight at 4 °C and with secondary antibody (goat anti-mouse, Li-COR, 926-68020, 1:20,000 and/or goat anti-rabbit, Li-COR, 926-32211, 1:20,000) for 1 hour at room temperature. Li-COR Odyssey Clx imaging system was used to image blots. Image Studio Software version 5.2 (Li-COR) was used for blot analysis. Antibodies used for western blotting included mouse monoclonal anti-Lamin A/C (E1) (Santa Cruz Biotechnology, sc-376248, 1:1000), rabbit polyclonal anti-AFF1 (Bethyl, A-3020344A-T, 1:500), rabbit polyclonal anti-AFF4 (Abclonal, A4644, 1:1000), rabbit polyclonal anti-HEXIM1 (Bethyl, A303-112A-T, 1:1000), rabbit monoclonal anti-ATF-3 (E9J4N) (Cell Signaling, 18665, 1:1000), rabbit monoclonal anti-DUSP1 (E8L7D) (Cell Signaling, 48625, 1:1000), mouse monoclonal anti-RhoE (RND3) (Cell Signaling, 3664, 1:300), Rabbit anti-PhosphoThr186 CDK9 (Cell Signaling, 2549, 1:1000), Rabbit anti-CDK9 (C12F7) (Cell Signaling, 2316, 1:1000), Rabbit anti-HA (C29F4) (Cell Signaling, 3724, 1:1000), Rabbit anti-DNMT1 (D63A6) (Cell Signaling, 5032, 1:1000), and Rabbit anti-MYC (D84C12) (Cell Signaling, 5605, 1:1000).
5
Immunoblot Analysis of Chromatin Remodeling Proteins
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For immunoblot analysis, 10–30 μg of cell lysate was loaded per lane for SDS-page and then transferred to PVDF membranes. Blots were blocked with Odyssey Blocker PBS (Li-COR) + 2.5% BSA at room temperature for 1 h. Blots were then incubated with primary antibody at 4°C overnight and then with secondary antibodies (IRDye 680LT goat anti-mouse, LI-COR, 926–68020, 1:15,000, and/or IRDye 800CW goat anti-rabbit, LI-COR, 926–32211, 1:15,000) at room temperature for 1 h. The blots were imaged using Li-COR Odyssey CLx (LI-COR). Primary antibodies used for western blotting include anti-PBRM1 (Bethyl, A700-019), anti-PIAS1 (D33A7) (Cell Signaling, 3550), anti-ARID2 (D8D8U) (Cell Signaling, 82342), anti-BRG1 (G-7) (Santa Cruz Biotechnology, sc-17796), anti-ARID1A (PSG3) (Santa Cruz Biotechnology, sc-32761), anti-BRD7 (Bethyl, A302-304A-T), anti-HA (C29F4) (Cell Signaling, 3724), and anti-Lamin A/C (E−1) (Santa Cruz Biotechnology, sc-376248).
6
Immunoblotting Antibody Dilutions
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The following antibodies were used for immunoblotting at the indicated dilution: Anti-LAMIN A/C (N-18) 1:100 (SantaCruz, Catalogue No: sc6215), Anti-LAMIN A/C (E-1) 1:100 (SantaCruz, Catalogue No: sc376248), anti-β actin 1: 600 (SantaCruz, Catalogue No: sc47778), anti-α tubulin 1: 750 (Calbiochem, Catalogue No: CP06). HRP-conjugated secondary antibodies were anti-goat 1:500 (Novex, Catalogue No: A15909), anti-mouse 1:1000 (Cell Signaling, Catalogue No: 7076)
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