Axio z2

Immediately following dissection, tumors were isolated and washed in PBS, prior to immersion in 30% sucrose overnight. After fixation in 4% neutral-buffered formalin, tumors were then embedded in Tissue-Tek® O.C.T. Compound (Sakura Finetek) and sectioned with a cryostat at 20 μm. Tumor sections were blocked and probed first with G6PD antibody (Abcam) followed by incubation with an HRP-conjugated secondary antibody (R&D Systems). The sections were then stained with DAB and counterstained with hematoxylin. For Ki67 immunohistochemistry, sections were incubated with anti-Ki67 antibody (Abcam), followed by immunostaining with a fluorescent-labeled secondary antibody (Invitrogen). Slides were counterstained using DAPI (Sigma-Aldrich). Finally, H&E staining was also performed as previously described (Fischer et al., 2008 ). Briefly, slides were immersed in a hematoxylin solution, followed by a series of rinses and counterstaining with eosin prior to application of a clear coverslip. These slides were imaged using color microscopy (Zeiss Axio Z2) or confocal microscopy (Leica SP8 Confocal).