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Polysorbate 20

Manufactured by Thermo Fisher Scientific
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Polysorbate 20 is a non-ionic surfactant commonly used in laboratory applications. It functions as a wetting agent, emulsifier, and dispersant to improve the solubility and stability of various compounds in aqueous solutions.

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Lab products found in correlation

Oleic acid, by Thermo Fisher Scientific (3 mentions) Sodium glycocholate, by Thermo Fisher Scientific (3 mentions) Leucine, by Thermo Fisher Scientific (2 mentions) Stearic acid, by Thermo Fisher Scientific (2 mentions) Toluene, by Thermo Fisher Scientific (1 mentions) 1 butanol, by Thermo Fisher Scientific (1 mentions) Potassium nitrate, by Thermo Fisher Scientific (1 mentions) Ferrocene, by Thermo Fisher Scientific (1 mentions) Menadione, by MP Biomedicals (1 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Cholesterol, by Merck Group (1 mentions) Transferrin, by Merck Group (1 mentions) D mannitol, by Thermo Fisher Scientific (1 mentions) Trifluoracetic acid, by Thermo Fisher Scientific (1 mentions) Remdesivir, by Alsachim (1 mentions) Gs 441524, by Alsachim (1 mentions) Lactose monohydrate, by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Hanks balanced salt solution hbbs, by Merck Group (1 mentions) Glass coverslip, by Thermo Fisher Scientific (1 mentions)

13 protocols using polysorbate 20

1

Electrochemical Detection of Menadione

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Polysorbate 20 (Acros, >95%), sodium dodecyl sulfate (SDS; Fisher, ≥ 99%), 1-butanol (Fisher, 99.98%), toluene (Fisher, 99.9%), potassium nitrate (Fisher, ≤ 3ppm heavy metals, iron), ferrocene (Alfa Aesar, 99%), and menadione (MP Biomedicals, ≥ 98%) were used as received. Chemical structures of surfactants and electroactive species are shown in Figure 1.
Barth B.A., Imel A., Nelms K.M., Goenaga G.A, & Zawodzinski T. (2022). Microemulsions: Breakthrough Electrolytes for Redox Flow Batteries. Frontiers in Chemistry, 10, 831200.
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2

Remdesivir Dry Powder Inhaler Formulation

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The Remdesivir for formulation was purchased from Medkoo Biosciences (Research Triangle Park, NC, USA). Remdesivir, GS-441524, and its heavy isotope internal standards were purchased from Alsachim (Illkirch-Graffenstaden, France). Lactose monohydrate, leucine, polysorbate 20, acetonitrile (HPLC grade), and trifluoracetic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA). D-Mannitol was bought from Acros Organic (Fair lawn, NJ, USA). Dipalmitoylphosphotidylcholine (DPPC) was purchased from Avanti Polar Lipid, Inc. (Alabaster, AL, USA). Cholesterol, albumin, transferrin, ascorbic acid and Hanks’ Balanced Salt solution (HBBS) were purchased from Sigma-Aldrich (St.Louis, MO, USA). Sulfobutylether-beta-cyclodextrin (SBECD, Captisol®) was kindly provided by CyDex Pharmaceuticals, Inc. (Lawrence, KS, USA) High-resistance Monodose RS00 dry powder inhalers were kindly provided by Plastiape S.p.A. (Osnago, Italy).
Sahakijpijarn S., Moon C., Koleng J.J., Christensen D.J, & Williams RO I.I.I. (2020). Development of Remdesivir as a Dry Powder for Inhalation by Thin Film Freezing. Pharmaceutics, 12(11), 1002.
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3

Aggregation Susceptibility of Monoclonal Antibody

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Humanized IgG1 monoclonal antibody (molecular weight 146 kDa), here denoted as “3M”, was provided by MedImmune (Gaithersburg, MD).37 (link) The antibody was obtained at a stock concentration of 150 mg/mL in 10 mM L-histidine at pH 6. The antibody 3M is a human IgG1 with three mutations (S239D/A330L/I332E) in the CH2 portion of the Fc. These mutations reduce the thermal stability of 3M,37 (link) which was chosen for the current work because of previous studies36 (link) that showed it to be prone to aggregation when exposed to silicone oil-water interfaces. Polysorbate 20 (>97% purity, Fisher BioReagents) was obtained from Fisher Scientific (Pittsburgh, PA). All buffer salts were of reagent grade or higher, and all solutions were prepared in de-ionized water filtered through a 0.22 μm Millipore filter (Billerica, MA). Silicone oil (Dow Corning 360, 100 cSt) was of medical grade and purchased from Nexeo Solutions (Denver, CO). The syringes used in the incubation studies were BD Hypak SCF 1mL long 27G1/2 (BD Medical-Pharmaceutical Systems, Franklin Lakes, NJ). Glass coverslips, Micro-90, and isopropyl alcohol were obtained from Fisher Scientific (Waltham, MA). Nickel TEM grids (EMS G100-Ni) were obtained from Electron Microscopy Sciences (Hatfield, PA). Teflon® rings were fabricated in-house at the University of Colorado-Boulder.
Gerhardt A., McUmber A.C., Nguyen B.H., Lewus R., Schwartz D.K., Carpenter J.F, & Randolph T.W. (2015). Surfactant Effects on Particle Generation in Antibody Formulations in Pre-filled Syringes. Journal of pharmaceutical sciences, 104(12), 4056-4064.
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4

Niclosamide Formulation Development

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Niclosamide was purchased from Shenzhen Nexconn Pharmatechs LTD. (Shenzhen, China). Tert-butanol (TBA), acetone, acetonitrile, leucine, polysorbate 20, trifluoracetic acid were acquired from Fisher Scientific (Pittsburgh, PA, USA). Pearlitol® PF-mannitol was purchased from Roquette America (Keokuk, IA, USA).
Jara M.O., Warnken Z.N., Sahakijpijarn S., Moon C., Maier E.Y., Christensen D.J., Koleng J.J., Peters J.I., Hackman Maier S.D, & Williams III R.O. (2021). Niclosamide inhalation powder made by thin-film freezing: Multi-dose tolerability and exposure in rats and pharmacokinetics in hamsters. International Journal of Pharmaceutics, 603, 120701.
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5

Lipid-based Nanoformulations for Drug Delivery

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RX-HCl, liquid lipids (capmul MCM C8, castor oil, and labrafil M 1944 CS), and surfactants (polysorbate 80, polysorbate 20, and cremophor EL) were purchased from Fisher Scientific (Hanover Park, IL USA). Solid lipids, glyceryl monostearate, compritol 888ATO, precirol ATO5, and dynasan 118 were gifted from Gattefossé (Paramus, NJ USA). Poloxamer 188 (Lutrol® F68) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and solvents used in the study were of HPLC grade and de-ionized water was used throughout the study.
Muhindo D., Ashour E.A., Almutairi M., Joshi P.H, & Repka M.A. (2021). CONTINUOUS PRODUCTION OF RALOXIFENE HYDROCHLORIDE LOADED NANOSTRUCTURED LIPID CARRIERS USING HOT-MELT EXTRUSION TECHNOLOGY. Journal of drug delivery science and technology, 65, 102673.
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6

IVIG Stability Assessment Protocol

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Gammagard® (IVIG), 100 mg/mL in 200 mM Glycine, pH 4.2 (Lot# LE12N107AB, Expiry: May 2016) manufactured by Baxter Healthcare Corporation (Westlake Village CA.) was used as the model protein in this study. Glycine, sodium phosphate, sodium chloride and polysorbate 20 were purchased from Fisher Scientific (Fair Lawn, NJ).
Pardeshi N.N., Zhou C., Randolph T.W, & Carpenter J.F. (2018). Protein nanoparticles promote microparticle formation in intravenous immunoglobulin solutions during freeze-thawing and agitation stresses. Journal of pharmaceutical sciences, 107(7), 1852-1857.
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7

Production and Characterization of Viral Glycoproteins

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Recombinant viral glycoproteins EBOV-GP, MARV-GP, and SUDV-GP were produced at the University of Hawaiʻi at Mānoa as previously described16 (link). Ammonium acetate was purchased from J.T. Baker (Avantor, Radnor, PA) and trehalose was generously donated from Pfanstiehl, Inc. (Waukegan, IL). FIOLAX® 3 mL alumino-borosilicate glass vials were obtained from Schott (Lebanon, PA), and 13 mm two-leg butyl rubber stoppers and 13 mm aluminum seals were purchased from DWK Life Sciences, LLC (Millville, NJ). Coomassie Brilliant Blue G-250 was purchased from Amresco (Solon, OH). Mini-Protean® TGX™ Gels, 4x Laemmli Sample Buffer, and Precision Plus Protein™ All Blue Standards were purchased from Bio-Rad Laboratories (Hercules, CA). The following materials were purchased from Sigma Aldrich (St. Louis, MO): 10x phosphate buffered saline (PBS), DL-dithiothreitol (DTT), sodium dodecyl sulfate (SDS), sodium sulfate, sodium phosphate dibasic, and acrylamide. Materials from Thermo Fisher Scientific (Waltham, MA) included polysorbate 20, tris(hydroxymethyl)aminomethane, glycine, and sodium phosphate monobasic.
Preston K.B., Monticello C.R., Wong T.A., To A., Donini O., Lehrer A.T, & Randolph T.W. (2020). Preservation of Quaternary Structure in Thermostable, Lyophilized Filovirus Glycoprotein Vaccines: A Search for Stability-Indicating Assays. Journal of pharmaceutical sciences, 109(12), 3716-3727.
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8

Methylene Blue Nanoparticle Synthesis

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Methylene blue (MB) dye 89%, Stearic acid 97%, oleic acid 90%, De-ionized water, polysorbate 20 (tween20), sodium glycocholate 97.5% and sodium tourocholate, 96% were purchased from Alfa Aesar (Thermo Fisher Scientific, Germany), while citronellal 93% and carvacrol 95% monoterpens were obtained from Acros Organics (Thermo Fisher Scientific, Germany). All the chemical reagents were used without any purification.
Radwan I.T., Sayed-Ahmed M.Z., Ghazawy N.A., Alqahtani S.S., Ahmad S., Alam N., Alkhaibari A.M., Ali M.S., Selim A, & AbdelFattah E.A. (2023). Effect of nanostructure lipid carrier of methylene blue and monoterpenes as enzymes inhibitor for Culex pipiens. Scientific Reports, 13, 12522.
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9

Isothermal DNA Amplification Reagents

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Betaine, calcein, KCl, MgSO4, MnCl2, (NH4)2SO4, and Triton X-100 were purchased from MilliporeSigma (St. Louis, MO). Bst 2.0 WarmStart DNA polymerase was purchased from New England Biolabs (Beverly, MA), deoxynucleotide triphosphates from Promega (Madison, WI), Tris (pH 7.5) from Invitrogen (Carlsbad, CA), Nuclease-free water, DMSO, NaOH, and polysorbate 20 from ThermoFisher (Waltham, MA). Tris (pH 8.8) was purchased from VWR (Radnor, PA), and PCR tubes with optically clear lid strips from Bio-Rad (Hercules, CA). Primers were purchased from Integrated DNA Technologies (Coralville, IA).
Barnes L., Heithoff D.M., Mahan S.P., Fox G.N., Zambrano A., Choe J., Fitzgibbons L.N., Marth J.D., Fried J.C., Soh H.T, & Mahan M.J. (2018). Smartphone-based pathogen diagnosis in urinary sepsis patients. EBioMedicine, 36, 73-82.
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10

Curcumin-Based Nanoformulation Development

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Curcumin (Cur) 97%, oleic acid 90%, stearic acid 97%, polysorbate 20 (tween 20), sodium taurocholate 96%, sodium glycocholate 97.5%, hydrogen peroxide 29%, and deionized water were procured from Alfa Aesar (Thermo Fisher Scientific, Dreieich, Germany), whereas linalool and geraniol were procured from Acros Organics (Thermo Fisher Scientific, Dreieich, Germany). Skweez (contains Imidacloprid 34%) insecticide was purchased locally from Semadak (Damanhur, El Beheira governorate Egypt). All chemical reagents were utilized without being purified, with the exception of Skweez insecticide, which was lyophilized to produce a powder.
Radwan I.T., Ghazawy N.A., Alkhaibari A.M., Gattan H.S., Alruhaili M.H., Selim A., Salem M.E., AbdelFattah E.A, & Hamama H.M. (2024). Nanostructure Lipid Carrier of Curcumin Co-Delivered with Linalool and Geraniol Monoterpenes as Acetylcholinesterase Inhibitor of Culex pipiens. Molecules, 29(1), 271.
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