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Tetrahydrolipstatin

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Tetrahydrolipstatin is a synthetic compound that inhibits the action of the enzyme lipase. It is used in laboratory research settings to study lipid metabolism and related biological processes.

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Lab products found in correlation

Devazepide, by Bio-Techne (1 mentions) Jzl184, by Bio-Techne (1 mentions) Sch23390, by Merck Group (1 mentions) Eticlopride, by Merck Group (1 mentions)

Close spelling variants of this product

Tetrahydrolipstatin (THL) (2 citations)

2 protocols using tetrahydrolipstatin

1

Pharmacological Validation of Lipase Assays

Cited 2 times
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AM6545, a peripherally-restricted CB1R neutral antagonist, was given by IP injection at 10 mg per kg (Northeastern University Center for Drug Discovery, Boston, MA, USA). Devazepide (Tocris, Bristol, UK), a CCKA receptor antagonist, was given IP at 0.3 mg per kg. Both drugs were dissolved in vehicle consisting of 7.5% DMSO, 7.5% Tween80, and 85% sterile saline, and warmed in a water bath to ensure solubility. All control conditions were matched, using vehicle in place of drugs and injections occurred 1 h prior to behavior recording (1,600 h). A 72-h washout period was allowed between drug treatments. JZL184 (Tocris, Bristol, UK), a potent inhibitor of monoacylglycerol lipase (MGL), was used to prevent monoacylglycerol hydrolysis in the diacylglycerol lipase (DGL) assay and to validate our MGL assay (described below). Tetrahydrolipstatin (Tocris, Bristol, UK), a lipase inhibitor used routinely to study DGL activity (Gregg et al., 2012 (link); Jung et al., 2012 (link)), was used to validate our DGL assay.
Argueta D.A., Perez P.A., Makriyannis A, & DiPatrizio N.V. (2019). Cannabinoid CB1 Receptors Inhibit Gut-Brain Satiation Signaling in Diet-Induced Obesity. Frontiers in Physiology, 10, 704.
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2

Dopamine and Endocannabinoid Receptor Antagonists

Cited 5 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The DA D1 receptor antagonist SCH23390 (SCH; 125941-87-9, Sigma-Aldrich) and the DA D2 receptor antagonist Eticlopride (97612-24-3, Sigma-Alrich) were each dissolved in aCSF to one of three doses (0.25μg/0.5μl; 0.5μg/0.5μl; 1.0 μg/0.5μl) for IC delivery. Similar doses of SCH diminish responding for positive reinforcers and do not result in locomotor impairments [48 (link), 61 –63 (link)]. The CB1 antagonist rimonabant (National Institute on Drug Abuse Drug Supply Program) was dissolved in a 1:1:18 mixture of ETOH, alkamuls (EL620, Rhodia Group), and 0.9%physiological saline to either of two doses (0.56mg/0.1ml; 1mg/0.1ml) for IV delivery. Rimonabant was dissolved in a 1:1:18 mixture of ETOH, alkamuls, and aCSF to one of two doses (0.2μg/0.5μl; 1.0μg/0.5μl) for IC delivery. Similar doses of rimonabant have been utilized by our laboratory to investigate food-maintained responding, and have not been found to affect locomotor behavior [39 (link), 64 (link)–67 (link)]. The diacylglycerol lipase inhibitor tetrahydrolipstatin (Tocris Bioscience) was dissolved in a 1:1:18 mixture of ETOH, alkamuls, and aCSF to a dose of 5μg/0.5μl or 0.5 μg/0.5μl [33 (link), 68 (link)].
Wenzel J.M., Oleson E.B., Gove W.N., Cole A.B., Gyawali U., Dantrassy H.M., Bluett R.J., Dryanovski D.I., Stuber G.D., Deisseroth K., Mathur B.N., Patel S., Lupica C.R, & Cheer J.F. (2018). Phasic dopamine signals in the nucleus accumbens that cause active avoidance require endocannabinoid mobilization in the midbrain. Current biology : CB, 28(9), 1392-1404.e5.
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