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Es500w ccd camera

Manufactured by Ametek
Sourced in Japan, Germany

The ES500W CCD camera is a high-performance imaging device designed for laboratory and scientific applications. It features a charge-coupled device (CCD) sensor that captures images with high resolution and sensitivity. The core function of the ES500W is to provide reliable and accurate image acquisition for various analytical and research purposes, without any interpretation or extrapolation on its intended use.

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5 protocols using es500w ccd camera

1

Characterizing AuNRs by Transmission Electron Microscopy

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AuNRs were deposited on carbon-coated copper grids (Ted Pella) and air-dried prior to TEM (JEOL JEM-2100 TEM operated at 200 kV). Images were recorded on a Gatan 1.35 K × 1.04 K ×12 bit ES500W CCD camera.
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2

Quantification of Mitochondrial Ultrastructure

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Cells were grown in SD medium. Once they reached OD600 ~ 0.5, 5 mL of a fixative solution (6% paraformaldehyde, 4% glutaraldehyde and Cacodylate buffer 0.2 M (pH = 7.4)) was added to 5 mL of media. The samples were gently rotated for 40 minutes at 30 °C, then centrifuged and washed twice in Cacodylate buffer and incubated again for an hour in the fixative solution. After washing with Cacodylate buffer and centrifugation, samples were embedded in 10% gelatin in water, fixed overnight with the fixative solution, washed in Cacodylate buffer, and then incubated overnight in 2.3 M sucrose and rapidly frozen in liquid nitrogen. Frozen ultrathin (70–90 nm) sections were cut with a diamond knife (Diatome AG, Biel, Switzerland) at –120 °C on an EM UC6 ultramicrotome (Leica Microsystems, Vienna, Austria). The sections were collected on 200-mesh Formvar-coated nickel grids. Contrasting and embedding were performed as previously described [46 (link)]. The embedded sections were observed in a Tecnai T12 electron microscope (FEI, Eindhoven, The Netherlands) operating at 120 kV. Images were recorded using an Erlangshen ES500W CCD camera (GATAN) or an Eagle 2 k × 2 k CCD camera (FEI). The quantification of mitochondrial area and cristae was performed using imageJ [47 ].
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3

Ultrastructural Analysis of Sciatic Nerve

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Sciatic nerves fixed in 2% glutaraldehyde/1% paraformaldehyde and post-fixed in 1% osmium tetroxide were Epon-embedded using routine procedures, and ultrathin sections were cut. Semi-thin sections (0.5 μM) were used for toluidine blue staining, and ultrathin sections were used for electron microscopy. The ultrathin sections were mounted on formvar-coated copper grids and contrasted with uranyl acetate and lead citrate. The samples were viewed in a Jeol JEM-2100 electron microscope at an acceleration voltage of 120 kV and a magnification range of 1,500× to 4,000×. Electron micrographs were recorded on a Gatan Erlangshen ES500W CCD camera.
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4

TEM Imaging of M. gryphiswaldense Cells and Magnetosomes

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For transmission electron microscopy (TEM) analyses of M. gryphiswaldense cells and isolated magnetosomes, specimens were deposited onto carbon-coated copper-mesh grids (Science Services, Munich, Germany). Isolated particles were additionally negatively stained with 2% uranyl acetate. TEM was performed on a JEOL 1400 (JEOL, Tokyo, Japan) with an acceleration voltage of 80 kV. Images were taken with a Gatan Erlangshen ES500W CCD camera.
For localization studies, neurons, both control and stretched, were fixed with 1.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, at pH 7.4. After rinses with the same buffer, cells were post-fixed in reduced osmium tetroxide solution (1% OsO4, 1% K3Fe(CN)6, and 0.1 M sodium cacodylate buffer), stained with our homemade staining solution [52 (link)], dehydrated in a growing series of ethanol, and flat-embedded in epoxy resin.
Ultra-thin sections (90 nm) were cut with a UC7 LEICA ultramicrotome (UC7—Leica Microsystems, Vienna, Austria) and collected on 300 mesh copper grids (EMS—Electron Microscope Science, Hatfield, PA, USA).
Images for morphological characterization have been collected with a Transmission electron microscope Zeiss LIBRA 120 Plus operating at 120 KeV equipped with an in-column omega filter.
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5

Quantifying Magnetite Biomineralization

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Transmission electron microscopy of whole cells was performed with specimens directly deposited onto carbon-coated copper grids (Science Services, Munich, Germany). For TEM imaging a Jeol Jem 1400 + (Freising, Germany) was operated at an acceleration voltage of 80 kV. Image acquisition was performed with a Gatan Erlangshen ES500W CCD camera. Average particle sizes were measured by data processing with ImageJ software package v1.52i. For quantitative assessment of magnetite particle biomineralization at different timepoints, specifically at the process start, in the mid-exponential growth phase and at end of growth 1000 magnetosome particles per triplicate were counted and combined for evaluation.
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