Mouse polyclonal antibodies against human BEX4 protein were generated in C57BL/6 mice. Briefly, purified GST-BEX4 protein was injected four times intraperitoneally. Rabbit polyclonal antibodies against C-terminal polypeptides 89–106 of human BEX4 protein were commercially generated (Youngin Frontier, Seoul, Korea). The other antibodies used in this study were as follows: anti-GFP, anti-PLK1, anti-CDK1, anti-aurora A, anti-cIAP-1, anti-cdc20 (Santa Cruz Biotechnology, Dallas, TX, USA), anti-actin (Sigma-Aldrich, St. Louis, MO, USA), anti-poly(ADP-ribose) polymerase-1 (PARP), anti-cleaved-caspase 9, cleaved-caspase 7, anti-active-caspase 3, anti-phospho-threonine (p-Thr) (Cell Signaling Technology, Danvers, MA, USA), anti-aurora B (BD Biosciences PharMingen, San Diego, CA, USA), anti-securin (PTTG1; Zymed, San Francisco, CA, USA), anti-Myc (Bethyl Laboratories, Montgomery, TX, USA), and Alexa Fluor (Invitrogen, Leek, The Netherlands). The following reagents were used: MG132, cycloheximide (CHX), dimethyl sulfoxide (DMSO) (AG Scientific, San Diego, CA, USA), nocodazole (Sigma-Aldrich), and PLK1 kinase inhibitor BI2536 (Axon Medchem, Groningen, Netherlands).
Anti ciap 1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-cIAP-1 is a laboratory reagent used for the detection and quantification of the cellular inhibitor of apoptosis protein-1 (cIAP-1). cIAP-1 is a protein that plays a role in the regulation of cell survival and cell death pathways. The Anti-cIAP-1 reagent can be used in various biological assays and research applications to study the expression and function of cIAP-1 in different cell types and experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Anti ciap2, by Santa Cruz Biotechnology (3 mentions)
Bca kit, by Thermo Fisher Scientific (3 mentions)
Anti actin, by Merck Group (2 mentions)
Mg132, by AG Scientific (2 mentions)
Nocodazole, by Merck Group (1 mentions)
Anti cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Anti active caspase 3, by Cell Signaling Technology (1 mentions)
Anti plk1, by Santa Cruz Biotechnology (1 mentions)
Anti cdk1, by Santa Cruz Biotechnology (1 mentions)
Anti aurora a, by Santa Cruz Biotechnology (1 mentions)
Anti cdc20, by Santa Cruz Biotechnology (1 mentions)
Anti poly adp ribose polymerase 1 parp, by Cell Signaling Technology (1 mentions)
Anti aurora b, by BD (1 mentions)
Anti myc, by Fortis Life Sciences (1 mentions)
Anti gfp, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 7, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by AG Scientific (1 mentions)
Bi2536, by Axon Medchem (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Ecl detection system, by Cytiva (1 mentions)
5 protocols using anti ciap 1
1
Generation and Characterization of Anti-BEX4 Antibodies
2
Western Blot Analysis of Apoptosis Regulators
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Whole-cell lysates were obtained from cultured cells with RIPA buffer (Boston Bioproducts Inc., Ashland, MA, USA) containing protease inhibitor cocktail (Roche, Basel, Switzerland) plus 10 mM NaF and 10 mM Na3VO4. Proteins were fractionated by 10% SDS-polyacrylamide gel and transferred to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were incubated with the following antibodies: Anti-A20 (Active Motif, Carlsbad, CA, USA); Anti-cIAP-1, Anti-cIAP-2, Anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); Anti-GAPDH (Millipore); Anti-XIAP (Transduction Laboratories, San Jose, CA, USA); and Anti-vinculin (Sigma). Following incubation of anti-IgG, the proteins were visualized using an ECL detection system (Amersham, Piscataway, NJ, USA).
3
Protein Expression Analysis Protocol
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The total cell lysates were prepared by resuspending 0.45×106 cells in 20–50 µl of RIPA lysis buffer (50 mM Tris buffer, 20 mM HEPES, 100 mM NaF; 120 mM NaCl, 0.5% Triton X-100, 100 µM Na3VO4, pH 7.6) Total lysates was quantified using a BCA kit (#23225; Thermo Fisher Scientific) according to the manufacturer's protocols. The proteins (30–70 µg) were isolated using 10 or 12% SDS-PAGE gels and electrotransferred onto NC membranes (GE Healthcare). Target proteins were identified using the respective antibodies and Immobilon Western Chemiluminescent HRP Substrate Solution (WBKLS0100; Millipore) and visualized by Davinch-Chemi (CAS-400SM; Davinch-K). Anti-PARP antibody (1:1,000; #9542) and anti-Bax (1:1,000; #2772) were obtain from Cell Signaling Technology. Anti-caspase-3 (1:3,000; ADI-AAP-113) and anti-FLIP (1:700; ALX-804-961-0100) were purchased from Enzo Life Sciences. Anti-Bcl-2 (1:700; sc-7832), anti-Bcl-xL (1:1,000, sc-634), anti-Mcl-1 (1:1,000; sc-12756), anti-cIAP1 (1:1,000; sc-7943), anti-cIAP2 (1:1,000; sc-517317) and anti-β-actin antibody (1:5,000; sc-47778) were supplied by Santa Cruz Biotechnology, and anti-XIAP (1:10,000; 610717) antibody was obtained from BD Biosciences.
4
Antibody Profiling for Immune Signaling
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The following antibodies were used for subsequent studies: anti-Pellino-1 (F-7), anti-Bcl2, anti-cIAP1, anti-cIAP2, anti-TRAF6 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-TLR1, anti-TLR2, anti-TLR3, anti-TLR4, anti-TLR5, anti-TLR7, anti-TLR8, and anti-TLR9 (IMGENEX, San Diego, CA, USA), anti-actin, anti-Flag M2 (Sigma, St. Louis, MO, USA), anti-p-p65, anti-p65, anti-Rel-B, anti-p52, anti-p50, anti-TAK1, anti-PARP, anti-caspase 3 active (Cas-3a), anti-caspase 7 active (Cas-7a) (Cell signaling Technology, Danvers, MA, USA), anti-RIP (BD Biosciences, San Diego, CA, USA), anti-Lamin B1 (Abcam, Cambridge, UK), anti-HA, and anti-Myc (Roche, Basel, Switzerland) antibodies.
The following reagents were used for TLRs agonists: 5 mg/ml Poly(I:C) (TLR3 agonist), 5 mg/ml LPS (TLR4 agonist), and 5 mg/ml ssRNA40 (TLR8 agonist) (InvivoGen, San Diego, CA, USA). Cisplatin and paclitaxel (Selleck Chemicals, Houston, TX, USA), 25 μM MG132, 100 μg/ml CHX, dimethyl sulfoxide (DMSO) (A.G. Scientific, San Diego, CA, USA), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma, St. Louis, MO, USA) were purchased.
The following reagents were used for TLRs agonists: 5 mg/ml Poly(I:C) (TLR3 agonist), 5 mg/ml LPS (TLR4 agonist), and 5 mg/ml ssRNA40 (TLR8 agonist) (InvivoGen, San Diego, CA, USA). Cisplatin and paclitaxel (Selleck Chemicals, Houston, TX, USA), 25 μM MG132, 100 μg/ml CHX, dimethyl sulfoxide (DMSO) (A.G. Scientific, San Diego, CA, USA), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma, St. Louis, MO, USA) were purchased.
5
Western Blot Analysis of Apoptosis Regulators
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Whole cell lysates were prepared, resolved and blotted as previously described [5 (link)]. Membranes were probed with the following primary antibodies: anti-PTEN (Cell Signaling, Beverly, MA, USA); anti-c-FLIP (Enzo Life Sciences, Exeter, UK); anti-cIAP-1 (Santa Cruz, Heidelberg, Germany); anti-Bcl-2 (Cell Signaling, Beverly, MA, USA); anti-TNFR-1 (Santa Cruz, Heidelberg, Germany); and anti-Caspase-8 (Millipore, Billerica, MA, USA) at 4°C overnight. Membranes were washed three times with 1X PBS + 0.05% Tween-20 before being incubated with the appropriate horseradish peroxidase (HRP)-tagged secondary antibody (GE Healthcare, UK). Immunolabelled proteins were detected using the Luminata Crescendo substrate (Millipore, Billerica, MA, USA). Membranes were reprobed with GAPDH primary antibody (ABD Serotec, UK) to ensure equal loading.
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