Fetal bovine serum (fbs)

Na₂HPO₄, NaH₂PO₄, KCl, HCl, NaOH, and PBS tablets were purchased from Sigma Aldrich (St. Louis, MO, USA). PBS tablets were dissolved in 200 mL deionized water. Chloroform (CHCl₃), tetrahydrofuran (THF), and absolute ethanol (EtOH) were bought from Merck KGaA (Darmstadt, Germany). The polymers monocarboxy terminated poly(N-isopropyl acrylamide) (PNIPAAM-COOH), poly(acrylic acid) (PAA), and the adhesion promoter poly(glycidyl methacrylate) (PGMA) were purchased and characterized from Polymer Source, Inc. (Dorval, QC, Canada). Here, PGMA with molecular weight (MW) of 17.500 g/mol was chosen, whereas PAA had a MW of 26.500 g/mol and PNIPAAM of 47.600 g/mol. The positive photoresist AZ5214e was obtained by MicroChemicals GmbH, (Ulm, Germany). Cell culture reagents such as McCoy’s 5A, FBS, trypsin, L-glutamine, pen/strep, and sterile water were obtained from Biochrome AG (Berlin, Germany). Phosphate buffer solutions (0.1 M; Na₂HPO₄/NaH₂PO₄) with pH ranges from 5.7 to 8.0 were prepared.
Saos-2 cells were donated by Prof. Wiesmann, TU Dresden, IfWW.

For in vitro experiments, the Burkitt lymphoma cell line Raji as well as the melanoma cell line 526-Mel were transduced with lentiviral vectors encoding a GFP-firefly luciferase cassette. GFP⁺ 526-Mel cells were additionally modified to stably express CD20. For in vivo studies, a mouse-adapted Raji^ffLuc cell line was used [37 (link)]. All tumor cell lines were cultured in RPMI 1640 (Biowest, Nuaillé, France) supplemented with 2 mM glutamine (Lonza, Basel, Switzerland) and 10% fetal bovine serum (FBS, Biochrome, Berlin, Germany). The human embryonic kidney cell line 293 T was cultured in Dulbecco’s modified Eagle medium (Biowest, Nuaillé, France) supplemented with 10% FBS.

The two human breast cancer cell lines MCF-7 (Merck KGaA, Darmstadt, Germany) and MDA-MB-231 (Merck KGaA, Darmstadt, Germany) were cultivated at 37 °C in 5% CO₂ and 90% humidity under sterile conditions. Both cell lines were grown in Dulbecco’s modified Eagle’s serum (DMEM, PAN-Biotech GmbH, Aidenbach, Germany) supplemented with 10% fetal bovine serum (FBS, Biochrome AG, Berlin, Germany) and 1% Penicillin-Streptomycin (PenStrep, Gibco, Carlsbad, CA, USA). The cell lines were tested to be free of mycoplasma.

Human hepatocellular carcinoma cells (HepG2/C3A, ATCC, ref. number CRL-10741) maintained in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO Life Technologies, Darmstadt, Germany), supplemented with 10% fetal bovine serum (FBS, Biochrome, Berlin, Germany), 1% of 200 mM L-glutamine (Biochrome), and 1% of antibiotics solution (Penicillin G:10.000 IE/mL/Streptomycin: 10 mg/mL; Biochrome, Berlin, Germany) were used. Cells were routinely incubated under a humidified atmosphere containing 5% CO₂ at 37 °C and were regularly subcultured every 2–3 days.

Human embryonic kidney 293 T cells, African green monkey COS-7 kidney cells and African green monkey Vero 76 kidney cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; PAN Biotech) supplemented with 10% fetal bovine serum (FBS; Biochrome), 100 U/ml penicillin and 100 μg/ml streptomycin (PAN Biotech), and 1% L-glutamine (PAN Biotech). The cells were grown in a humidified atmosphere at 37°C and 5% CO₂.

Peripheral blood mononuclear cells (PBMCs) were collected from three to five (indicated in each figure legend) healthy human blood donors by venipuncture in EDTA-coated vacutainer tubes (Sarstedt). Due to the fact that blood was only obtained from the authors, according to our local ethics committee (University of Freiburg Ethics Committee), under the relevant national and local regulations, ethical approval and informed consent was not needed. PBMCs were separated from other blood components by Ficoll-Plaque (GE Healthcare Life Sciences) density gradient centrifugation and resuspended in MACs buffer containing anti-CD14 microbeads (Miltenyi Biotec). The isolation was performed via positive selection using the MS MACs Column (Miltenyi Biotec) and the MiniMACs magnet (Miltenyi Biotec) according to the manufacturer’s protocol. The CD14+ monocytes were counted and seeded at a density of 50,000 cells/ml in RPMI-1640 cell medium (Sigma Aldrich) containing 10% FBS (Bio Chrome) and PenStrep (Life Technologies Corporation). The CD14+ monocytes were treated with maturation factors GM-CSF (10 ng/mL, Peprotech) or M-CSF (25 ng/mL, Peprotech) to induce M1 or M2 macrophages, while M0 macrophages were left untreated. The cell suspensions were placed in T25 flasks (Greiner Bio-One) for two days.