For in vivo treatment of mice with the exosome maturation inhibitor GW4869 (Sigma), 8-12 weeks old C57BL/6N mice were injected intraperitoneally with GW4869 (0.3125 or 1.25µg per gram bodyweight) or carrier control once per day for five consecutive days. On day six, mice were intraperitoneally infected with 1x106L. monocytogenes (strain LO28). Bacterial inoculum was prepared as previously described 45 (link). At 24 hours p.i., mice were sacrificed and spleens were harvested. For cfu assays, livers and spleens were weighed, homogenized in PBS, and 1:10 serial dilutions were plated on Oxford agar plates (Merck). Colonies were counted after approximately 30 hours at 37°C. Some mice were treated with ODN1826 (InvivoGen) at a dose of 10 μg per mouse. Animals were housed under specific pathogen-free conditions according to FELASA guidelines and animal experiments have been approved by the Vienna University of Veterinary Medicine institutional ethics committee and performed according to protocols approved by the Austrian law (BMWF 68.205/0032-WF/II/3b/2014).
Oxford agar plates
Manufactured by Merck Group
Sourced in Germany
Oxford agar plates are a type of laboratory equipment used for microbiological culturing and analysis. They provide a solid growth medium for the cultivation of various microbial organisms, such as bacteria and fungi. The plates are pre-prepared with a nutrient-rich agar substrate, which supports the growth and isolation of microbes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using oxford agar plates
1
In vivo Treatment of Mice with Exosome Inhibitor
2
In vivo Treatment of Mice with Exosome Inhibitor
3
Selective Enrichment and Detection of Listeria and Salmonella
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the selective enrichment of Listeria spp. 1 ml of cell suspension was added to 9 ml Buffered-Listeria-Enrichment-Broth (BLEB) (Merck KGaA, Darmstadt, Germany). After 2 h of preincubation at 30°C Listeria-Selective-Enrichment supplement (Merck KGaA, Darmstadt, Germany) was added and the culture was further incubated at 30°C. After 24 h, 48 h, and 7 days 0.1 ml of the enrichment culture were transferred to 10 ml Half-Fraser-Bouillon (Merck KGaA, Darmstadt, Germany) and incubated aerobically at 37°C. Enrichment culture dependent detection of Listeria spp. was conducted after 24 and 48 h using Oxford-Agarplates (Merck KGaA, Darmstadt, Germany) and Palcam-Agarplates (Merck KGaA, Darmstadt, Germany) inoculated with the second enrichment culture. In case of a detection of Listeria spp. Rapid‘L.mono medium (Bio-Rad Laboratories GmbH, Munich, Germany) plates were used to identify L. monocytogenes. After inoculation, the identification plates were incubated at 37°C for 48 h.
In order to enrich S. enterica cells, 1 ml of cell suspension was added to 9 ml of BPW and incubated aerobically 37°C for 24 h. Afterwards cultures were streaked out on Xylose-Lysine-Deoxycholat (XLD) agar plates (Fluka, Buchs, Switzerland) and incubated at 37°C for 24 h in order to identify Salmonella spp.
In order to enrich S. enterica cells, 1 ml of cell suspension was added to 9 ml of BPW and incubated aerobically 37°C for 24 h. Afterwards cultures were streaked out on Xylose-Lysine-Deoxycholat (XLD) agar plates (Fluka, Buchs, Switzerland) and incubated at 37°C for 24 h in order to identify Salmonella spp.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!