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Ret 3

Manufactured by Physitemp
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The RET-3 is a laboratory equipment designed for temperature measurement. It features three channels for simultaneous monitoring of temperatures. The device is capable of displaying the measured values on a digital display.

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25 protocols using ret 3

1

Thermoneutral Acclimation and Cold Exposure

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Mice were pre-acclimated at thermoneutrality (28-30°C) for 1wk and then shifted to 4°C. Body temperature was measured with a rectal probe (Physitemp, RET3) and a reader (Physitemp, BAT-12) and implanted temperature probes (Bio Medic Data Systems, IPTT-300) with a transponder (Bio Medic Data Systems, DAS-7007R)34 ,35 .
Hu B., Jin C., Zeng X., Resch J.M., Jedrychowski M.P., Yang Z., Desai B.N., Banks A.S., Lowell B.B., Mathis D, & Spiegelman B.M. (2020). γδT cells and adipocyte IL-17RC control fat innervation and thermogenesis. Nature, 578(7796), 610-614.
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2

Measuring Mouse Temperature and Drug Discrimination

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Temperature was measured by inserting a probe (RET-3) (Physitemp Instruments, Inc., Clifton, NJ) attached to a microcomputer thermometer (7001H) (Physitemp Instruments, Inc., Clifton, NJ) 2 cm into the rectum. For drug discrimination, mice were placed in ventilated, sound-attenuating mouse operant chambers (MedAssociates, St. Albans, VT) with a house light located in the ceiling. On one wall were three holes spaced 5.5 cm apart (2.2 cm diameter each); each hole contained a photo beam and a light, and the center of each hole was 1.6 cm from the floor. In the center of the opposite wall was a fourth hole (also 2.2 cm diameter, center 1.6 cm from the floor) containing a dipper containing 0.01 cm3 condensed milk. An interface (MED-SYST-8, MedAssociates) connected the operant chambers to a computer running Med-PC software (MedAssociates), which controlled and recorded all experimental events.
Moerke M.J., de Moura F.B., Koek W, & McMahon L.R. (2016). Effects of nicotine in combination with drugs described as positive allosteric nicotinic acetylcholine receptor modulators in vitro: discriminative stimulus and hypothermic effects in mice. European journal of pharmacology, 786, 169-178.
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3

Measuring Mouse Body Temperature

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Mice were either housed at room temperature or pre-acclimatized at thermoneutrality (28–30 °C) for 1 week before being shifted to 4°C. Body temperature was measured with and a microprobe thermometer (Physitemp, BAT-12) equipped with a rectal probe (Physitemp, RET3).
Sun Y., Rahbani J.F., Jedrychowski M.P., Riley C.L., Vidoni S., Bogoslavski D., Hu B., Dumesic P.A., Zeng X., Wang A.B., Knudsen N.H., Kim C.R., Marasciullo A., Millán J.L., Chouchani E.T., Kazak L, & Spiegelman B.M. (2021). Mitochondrial TNAP Controls Thermogenesis by Hydrolysis of Phosphocreatine. Nature, 593(7860), 580-585.
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4

Measuring Mouse Thermoregulation

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Temperatures were taken 3 times during the light period (~10:00, 13:00 and 16:00) rectally using a probe (Physitemp RET-3) attached to a thermometer (Physitemp BAT-12) [43 (link)]. Mean temperatures for individual mice were then averaged by genotype. To test effects of cold, mice were housed individually at 4°C for 24 h under standard light/dark conditions [44 (link)].
Krois C.R., Vuckovic M.G., Huang P., Zaversnik C., Liu C.S., Gibson C.E., Wheeler M.R., Obrochta K.M., Min J.H., Herber C.B., Thompson A.C., Shah I.D., Gordon S.P., Hellerstein M.K, & Napoli J.L. (2019). RDH1 suppresses adiposity by promoting brown adipose adaptation to fasting and re-feeding. Cellular and molecular life sciences : CMLS, 76(12), 2425-2447.
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5

Thermoneutral Acclimation and Cold Exposure

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Mice were pre-acclimated at thermoneutrality (28-30°C) for 1wk and then shifted to 4°C. Body temperature was measured with a rectal probe (Physitemp, RET3) and a reader (Physitemp, BAT-12) and implanted temperature probes (Bio Medic Data Systems, IPTT-300) with a transponder (Bio Medic Data Systems, DAS-7007R)34 ,35 .
Hu B., Jin C., Zeng X., Resch J.M., Jedrychowski M.P., Yang Z., Desai B.N., Banks A.S., Lowell B.B., Mathis D, & Spiegelman B.M. (2020). γδT cells and adipocyte IL-17RC control fat innervation and thermogenesis. Nature, 578(7796), 610-614.
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6

Cold Exposure Impacts Marrow Adiposity

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At 8 or 52 weeks of age, 10 male C3H/HeJ (Jackson Labs, Stock: 000659) mice were placed individually into pre-cooled cages with bedding, food, and water, in a room held at 18°C. Littermate control mice (n = 10/strain) were held in identical conditions at room temperature (~22°C). After 1 week at 18°C, the cold room was adjusted to 4°C and maintained at this temperature for an additional 3 weeks. Control mice were held at 22°C for a total of 4 weeks (concurrently). Rectal core body temperature of control and cold-exposed mice was monitored daily using a Type T thermocouple rectal probe (RET-3, Physitemp Instruments, Inc., Clifton, NJ, USA) with a MicroTherma 2T hand held thermometer (ThermoWorks, Inc., Lindon, UT; Cat: THS-227-193). After 4 weeks, all mice were sacrificed and tissues were collected for analysis. Given the length of the intervention (21 days), we pre-scanned the non-decalcified tibia bones to calculate the marrow volume. After decalcification and osmium stain, the bones were re-scanned and the MAT volume was normalized to marrow volume in each region of interest to correct for any changes in the size of the tibiae between groups.
Scheller E.L., Doucette C.R., Learman B.S., Cawthorn W.P., Khandaker S., Schell B., Wu B., Ding S.Y., Bredella M.A., Fazeli P.K., Khoury B., Jepsen K.J., Pilch P.F., Klibanski A., Rosen C.J, & MacDougald O.A. (2015). Region-specific variation in the properties of skeletal adipocytes reveals regulated and constitutive marrow adipose tissues. Nature Communications, 6, 7808.
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7

Mice Acclimation to Cold Exposure

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Mice were pre-acclimated at thermoneutrality (28–30°C) for two weeks and then shifted to 4°C. Body temperature was measured with a rectal probe (Physitemp, RET3) and a reader (Physitemp, BAT-12).
Zeng X., Ye M., Resch J.M., Jedrychowski M.P., Hu B., Lowell B.B., Ginty D.D, & Spiegelman B.M. (2019). Innervation of Thermogenic Adipose Tissue via a Calsyntenin-3β/S100b Axis. Nature, 569(7755), 229-235.
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8

Indirect Calorimetry and Activity Monitoring in Obese Mice

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VO2 was measured using an indirect calorimetry system (Oxymax, Colombus Instrument OH) as described previosuly21 (link). HFD-induced obese mice were individually placed in the small acryl calorimeter chamber with free access to HFD and water from experiment day 12 to the end of experiment. After 12 h of adaptation, VO2 was estimated in individual mouse for 1 min at a 15-min interval over a 24 h period at airflow of 0.71/min. VO2 values were shown as ml/h/mouse. Locomotor activity of each mouse was monitored as numbers of infrared beam broken in both X and Y directions using an activity monitoring system (ATIMO-100 N, Shinfactory, Fukuoka, Japan). Rectal body temperature was measured using a thermometer (BAT-12R and RET-3, Physitemp).
Yoshino S., Iwasaki Y., Matsumoto S., Satoh T., Ozawa A., Yamada E., Kakizaki S., Trejo J.A., Uchiyama Y., Yamada M, & Mori M. (2020). Administration of small-molecule guanabenz acetate attenuates fatty liver and hyperglycemia associated with obesity. Scientific Reports, 10, 13671.
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9

Cold Tolerance Assay in Mice

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Mice were acclimatized at 30 °C for 4 weeks. The mice were then housed individually and acutely exposed to cold (4 °C). Rectal temperature was measured hourly using a digital thermometer (MicroTherma 2 T, Thermoworks) and a rectal probe (RET-3, Physitemp) for up to 8 hours. The end point was a 10 °C drop in temperature (approximately 27 °C) and mice were immediately euthanized.
Nam M., Akie T.E., Sanosaka M., Craige S.M., Kant S., Keaney Jr J.F, & Cooper M.P. (2017). Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression. Scientific Reports, 7, 2013.
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10

Mouse Rectal Temperature Measurement

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Mouse body temperature was measured between 9 and 11 a.m. Rectal temperature was measured with a digital thermometer (BAT‐12, Physitemp Instruments Inc., Clifton, NJ) equipped with a rectal probe (RET‐3, Physitemp Instruments Inc.) inserted to a depth of approximately 1.5 cm. The mice were lightly restrained during the insertion of the probe. To reduce methodological stress, mice were sufficiently acclimated to the procedure before the test period. All measurements were recorded when rectal temperature reading reached a plateau.
Yoshino N., Takeshita R., Kawamura H., Sasaki Y., Kagabu M., Sugiyama T., Muraki Y, & Sato S. (2017). Mast cells partially contribute to mucosal adjuvanticity of surfactin in mice. Immunity, Inflammation and Disease, 6(1), 117-127.
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