Inova 500 mhz

Manufactured by Bruker

Sourced in Germany

The INOVA 500 MHz is a nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It operates at a frequency of 500 MHz and is capable of performing various NMR experiments to analyze the structure and properties of chemical compounds.

Automatically generated - may contain errors

Lab products found in correlation

Waters 2487 dual λ absorbance detector, by Waters Corporation (1 mentions) Waters 515 pump, by Waters Corporation (1 mentions) Jascop650 spectrometer, by Jasco (1 mentions) Avance 3 hd 600 mhz, by Bruker (1 mentions) P 2000 polarimeter, by Jasco (1 mentions) Gf254 plates, by Qingdao Marine Chemical (1 mentions) 1260 infinity 2, by Agilent Technologies (1 mentions) Carbonic anhydrase from bovine erythrocytes, by Merck Group (1 mentions) Cary 60, by Agilent Technologies (1 mentions) Siliaflash f60, by Silicycle (1 mentions) Iso h2s 2, by World Precision Instruments (1 mentions) Ascend 400 mhz, by Bruker (1 mentions) Ct dna, by Merck Group (1 mentions) Avance 400 mhz, by Bruker (1 mentions) Av400, by Agilent Technologies (1 mentions) Accutof 4g, by JEOL (1 mentions) Emxplus spectrometer, by Bruker (1 mentions) Mercury 300 mhz, by Agilent Technologies (1 mentions) Avance 3 500 mhz, by Bruker (1 mentions) Ir cod cl 2, by Strem Chemicals (1 mentions)

Close spelling variants of this product

UNITY INOVA 500 MHz Inova 500

6 protocols using inova 500 mhz

Phytochemical Analysis of Natural Products

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optical rotations were measured on P2000 polarimeter (JASCO, Tokyo, Japan). UV spectra were obtained on a JASCOP650 spectrometer (JASCO). IR spectra were recorded on a Nicolet 5700 FT-IR microscope instrument (FT-IR microscope transmission, Thermo Electron Corporation, Madison, WI, USA). 1D and 2D NMR spectra were acquired at 500 or 600 MHz for ¹H and 125 or 150 MHz for ¹³C, respectively, on Varian INOVA 500 MHz, or Bruker AVANCE III HD 600 MHz (Bruker Corporation, Karlsruhe, Germany), in acetone-d₆ or methanol-d₄, with solvent peaks as references. ESI-MS and HR-ESI-MS data were measured using an AccuToFCS JMST100CS spectrometer (Agilent Technologies, Ltd., Santa Clara, CA, USA). Column chromatography (CC) was performed with silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China). HPLC separation was performed on an instrument consisting of a Waters 515 pump and a Waters 2487 dual λ absorbance detector (Waters Corporation, Milford, MA, USA) with a YMC semi-preparative column (250 × 10 mm i.d.) packed with C18 (5 μM). TLC was carried out with glass precoated silica gel GF254 plates (Qingdao Marine Chemical, Inc., Qingdao, China). Spots were visualized under UV light or by spraying with 7% H₂SO₄ in 95% EtOH followed by heating.

Zhang J.Q., Li G.P., Kang Y.L., Teng B.H, & Yao C.S. (2017). Biomimetic Synthesis of Resveratrol Trimers Catalyzed by Horseradish Peroxidase. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(5), 819.

+ Open protocol

+ Expand

Synthesis and Characterization of Peroxynicotinoid Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents were purchased from Sigma-Aldrich, Tokyo Chemical Industry (TCI), Fisher Scientific, and VWR and used directly as received. Carbonic anhydrase (CA) from bovine erythrocytes was purchased from Sigma-Aldrich (C2624). Silica gel (SiliaFlash F60, Silicycle, 230–400 mesh) was used for column chromatography. Deuterated solvents were purchased from Cambridge Isotope Laboratories (Tewksbury, Massachusetts, USA). ¹H, ¹⁹F and ¹³C{¹H} NMR spectra were recorded on Varian INOVA 500 MHz, Bruker 500 MHz, or Bruker 600 MHz NMR instruments at the indicated frequencies. Chemical shifts are reported in ppm relative to residual protic solvent resonances. H₂S Detection was monitored by using an H₂S electrode (ISO-H₂S-2, World Precision Instruments, Inc. Sarasota, Florida, USA) or UV-Vis spectrometer (Cary 60, Agilent Technologies, Santa Clara, California, USA) by following methylene blue assay in PBS buffer. Stability test was performed on HPLC (1260 Infinity II, Agilent Technologies, Santa Clara, California, USA). OA-PeroxyTCM-1, OA-PeroxyTCM-2, OA-PeroxyTCM-3, and OA-TCM-1 were synthesized by following our previous study.⁶⁹

Zhao Y., Henthorn H.A, & Pluth M.D. (2017). Kinetic Insights into Hydrogen Sulfide (H2S) Delivery from Caged-Carbonyl Sulfide (COS) Isomeric Donor Platforms. Journal of the American Chemical Society, 139(45), 16365-16376.

+ Open protocol

+ Expand

Characterization of Synthetic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents and chemicals were purchased from Merck. ctDNA (Deoxyribonucleic acid sodium salt from calf thymus DNA) were purchased from Sigma.
Melting points are uncorrected and were measured on a Stuart SMP3 apparatus. IR spectra of products were recorded using the ALPHA II Compact FT-IR spectrometer on KBr disks. The NMR (¹H and ¹³C) spectroscopy was performed using a Varian-INOVA 500 MHz and Bruker Ascend-400 MHz spectrometer. The mass spectra of products were recorded on an Agilent Technology (HP 5975C MSD) mass spectrometer operating at an ionization potential of 70 eV.
The UV–Vis absorption spectra were measured using a PerkinElmer apparatus with 1 cm quartz cuvette.

Ebadi A., Najafi Z., Pakdel-yeganeh H., Dastan D, & Chehardoli G. (2022). Design, synthesis, molecular modeling and DNA-binding studies of new barbituric acid derivatives. Journal of the Iranian Chemical Society, 19(9), 3887-3898.

+ Open protocol

+ Expand

Synthesis and Characterization of Iminosugars

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals and solvents were
used at the highest degree of purity and without further purification
(Sigma-Aldrich, Alfa Aesar, VWR). Thin-layer chromatography analysis
was carried out to follow the reaction course by using F254 Merck
silica gel plates and subsequent exposure to ultraviolet radiation,
iodine vapor, and spraying with ethanolic p-anisaldehyde
solution. Intermediates and final products were purified by column
chromatography with silica gel (70–230 mesh, Merck Kiesegel
60) and characterized by NMR analysis (NMR spectrometers: Varian Inova
500 MHz and Bruker AVANCE 400 MHz). Matrix-assisted laser desorption/ionization
(MALDI) mass spectrometry (MS) analysis was performed with an AB SCIEX
TOF/TOF 5800 MALDI mass spectrometer working in high-resolution reflectron
mode. Optical rotations were measured at 25 ± 2 °C in the
stated solvent. Iminosugars ent-2–6 were synthesized as previously reported.^{39 (link),40 (link)} All compounds were herein converted into the corresponding hydrochloride
salt by addition of 1 M HCl (1.0 equiv) followed by evaporation of
volatiles. Absolute quantitative nuclear magnetic resonance (qNMR)
experiments were performed to assess the purity of compounds following
the “general guidelines for quantitative 1D ¹H NMR
(qHNMR) experiments”, provided by the Journal of Medicinal
Chemistry. In all cases, purity was ≥95% (see the Supporting Information for details).

De Pasquale V., Esposito A., Scerra G., Scarcella M., Ciampa M., Luongo A., D’Alonzo D., Guaragna A., D’Agostino M, & Pavone L.M. (2023). N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome. Journal of Medicinal Chemistry, 66(3), 1790-1808.

+ Open protocol

+ Expand

Cobalt-Porphyrin Catalyzed Alkyne Functionalization

Check if the same lab product or an alternative is used in the 5 most similar protocols

All manipulations were performed under a nitrogen atmosphere using standard Schlenk techniques. All solvents used for catalysis were dried over and distilled from sodium (toluene) or CaH₂ (dichloromethane, hexane, ethyl acetate, methanol). All the cobalt-porphyrin catalysts [Co^II(P1)], [Co^II(P2)] [Co^II(P3)], and N tosylhydrazone sodium salts were synthesized according to published procedures.^{7a (link),8 (link),11 (link)} All the alkynes were used as purchased from Aldrich. All other chemicals were purchased from commercial suppliers and used without further purification. NMR spectra (¹H and ¹³C) were measured on a Varian INOVA 500 MHz (125 MHZ for ¹³C), a Bruker AV400 (100 MHZ for ¹³C) or a Varian MERCURY 300 MHz (75 MHZ for ¹³C) spectrometer. Mass spectra of the newly synthesised compounds were recorded in Agilent-5973 GC-MS spectrometer the corresponding HRMS data were recorded on JEOL AccuTOF 4G via direct injection probe. Elemental analysis of the newly synthesized complexes were performed by the Mikroanalytisches Laboratorium Kolbe, Germany. EPR spectra were recorded on a Bruker EMXplus spectrometer.

Paul N.D., Mandal S., Otte M., Cui X., Zhang X.P, & de Bruin B. (2014). A Metalloradical Approach to 2H-Chromenes. Journal of the American Chemical Society, 136(3), 1090-1096.

+ Open protocol

+ Expand

Air-Sensitive Organometallic Compound Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All air and moisture sensitive compounds were manipulated in a glovebox under a nitrogen atmosphere. Solvents for air and moisture sensitive reactions were vacuum transferred from sodium benzophenone ketyl (THF, Et₂O, pentane, d_6-benzene and d₈-toluene) or predried by passing through activated alumina columns of a Pure Process Technology solvent purification system. Li(NP)[46 ], Ni(COD)₂[50 ], 2,6-xylyl isocyanide[51 (link)] and TaCl₅(THF)[52 (link)] were prepared according to literature procedure. Pd(P^tBu₃)₂, [Rh(COD)Cl]₂, and [Ir(COD)Cl]₂ were purchased from Strem Chemicals and used without further purification. ¹H and ³¹P spectra were recorded on Varian INOVA 500 MHz, Bruker Avance III 400 MHz, or Bruker AVANCE III 500 MHz spectrometers. Complexes 2-8 are quite insoluble, which prevented proper ¹³C NMR characterization. Chemical shifts are reported with respect to residual protio solvent impurity for ¹H (s, 7.16 ppm for C₆D₅H), a PPh₃ standard for ³¹P (s, −6 ppm in C₆D₆), and solvent carbons for ¹³C (p, 67.21 ppm for THF-d₈). Attempts at elemental analysis of these air-sensitive complexes were unsuccessful.

Dunn P.L., Beaumier E.P, & Tonks I.A. (2020). Synthesis and Characterization of Tantalum-Based Early-Late Heterobimetallic Complexes Supported by 2-(diphenylphosphino)pyrrolide Ligands. Polyhedron, 181, 114471.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!