Species identification was based on morphological characteristics of the ascomata or conidiomata produced on infected host materials. The macro-morphological characteristics including structure and size of stromata; the size, color, and shape of discs; number and diameter of ostioles per disc; presence and absence of conceptacle were determined under a Leica stereomicroscope (M205). The micro-morphological characteristics including size and shape of conidiophores and conidia were determined under a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high-definition color camera with differential interference contrast (DIC). Over 10 ascomata/conidiomata were sectioned, and 10 asci and 30 ascospores/conidia were selected randomly for measurement. Colony morphology and growth rates were recorded and colony colors were described after 1 or 2 weeks according to the color charts of Rayner (1970 ). Adobe Bridge CS v.6 and Adobe Photoshop CS v.5 were used for the manual editing. Taxonomic novelties and descriptions were deposited in MycoBank (Crous et al., 2004 ).
Bridge cs v 6
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Lab products found in correlation
Eclipse 80i microscope, by Nikon (8 mentions)
M205 fa stereomicroscope, by Leica (4 mentions)
Digital sight ds ri2 high definition colour camera, by Nikon (4 mentions)
Nis elements d package v 3, by Nikon (3 mentions)
M205 stereomicroscope, by Leica (2 mentions)
Digital sight ds ri2, by Nikon (1 mentions)
Eclipse 80i compound microscope, by Nikon (1 mentions)
9 protocols using bridge cs v 6
1
Comprehensive Fungal Identification Protocol
2
Identification of Fungal Species
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Species identification was based on morphological features of the fruiting bodies, which was produced on the infected plant tissues, including stromata (arrangement and size), conceptacle (presence or absence), conidiomata (color, size, and shape), ostioles (number and diameter), locules (number and arrangement type), conidiophores, and conidia (size and shape), supplemented by cultural characteristics such as colony color, texture, and the presence or absence of airborne hyphae (Mostert et al., 2001 (link); Zhang et al., 2007 ; Zhu et al., 2020 (link)). The morphological features were observed under a Leica stereomicroscope (M205 FA) (Leica microsystem, Wetzlar, Genmany). Micro-morphological observations determined by a Nikon Eclipse 80i compound microscope. Measuring 30 conidiomata/ascomata and 50 conidia/ascospores, determined by length, width, and length/width ratio (L/W ratio). Recording the colony diameters and describing the color was based on the color charts of Rayner (1970) after 1–2 weeks on PDA in darkness. The results were edited manually by Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5.
3
Fungal Identification Protocol Using Morphology
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Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Cross-sections were prepared by hand using a double-edge blade under a dissecting microscope. More than 10 conidiomata/ascomata, 10 asci and/or 50 conidia/ascospores were measured to calculate the mean size and standard deviation (SD ). Microscopic photographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC ) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ). Colony diameters were measured and the colony colours described after 3 weeks according to the colour charts of Rayner (1970) .
4
Fungal Morphological Characterization
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Descriptions were performed based on morphological features of the ascomata or conidiomata from infected host materials. The macro-morphological photographs were captured using a Leica stereomicroscope (M205 FA) (structure and size of stromata, structure and size of ectostromatic disc and ostioles). Micro-morphological observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Over 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the color charts of Rayner (1970) . Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ).
5
Cytospora Morphological Characterization
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Observation and description of Cytospora species were based on morphological characteristics of the fruiting bodies produced on infected host materials including arrangement and size of stromata; presence and absence of a conceptacle; size, color, and shape of discs; and number and diameter of ostioles per disc. The morphological characteristics were determined under a Leica stereomicroscope (M205). Micro-morphological observations include size and shape of conidiophores and conidia determined under a Nikon Eclipse 80i microscope. Over 30 conidiomata were sectioned, and 50 conidia were selected randomly for measurement. Incubation was done on PDA at 25°C in darkness, and colony diameters and colors were recorded and described after 1 or 2 weeks according to the color charts of Rayner (1970) . Adobe Bridge CS v.6 and Adobe Photoshop CS v.5 were used for the manual editing. Taxonomic novelties were deposited in MycoBank (Crous et al., 2004 ).
6
Morphological Identification of Fungal Species
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Species identification was based on morphological features of the ascomata or conidiomata from infected host materials and micromorphology, supplemented by cultural characteristics. Microscopic photographs (structure and size of stromata; structure and size of ectostromatic disc and ostioles) were captured using a Leica stereomicroscope (M205 FA) (Leica Microsystems, Wetzlar, Germany). Microscopic observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope (Nikon Corporation, Tokyo, Japan), equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC ) illumination. The Nikon software NIS-Elements D Package v. 3.00, Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. More than 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured by Nikon software NIS-Elements D Package v. 3.00 to calculate the mean size/length and respective standard deviations (SD ). Colony diameters were measured and the colony features were described using the colour charts of Rayner (1970) .
7
Morphological Characterization of Fungal Strains
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Species identification was based on morphological features of fruiting bodies and micromorphology supplemented by cultural characteristics. Macro-morphological observations including structure and size of stromata, ectostromatic disc, and ostioles were determined using a Leica stereomicroscope (M205 FA) (Leica Microsystems, Wetzlar, Germany). Micro-morphological photographs were captured using a Nikon Eclipse 80i microscope (Nikon Corporation, Tokyo, Japan), including conidiophores, asci, and conidia/ascospores. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for manual editing. At least 10 conidiomata/ascomata, 10 asci, and 30 conidia/ascospores were randomly selected for measurement to calculate the mean width/length and respective standard deviations (SD). Cultural characteristics of strains incubated in the dark at 25°C were recorded. Colony morphology was described using the color charts of Rayner (1970) . Nomenclatural novelties were deposited in the MycoBank (1 Crous et al., 2004 ).
8
Fungal Identification and Characterization
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Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Cross-sections were prepared by hand using a double-edge blade under a dissecting microscope. At least 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size and standard deviation (SD). Microscopic photographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ). Colony diameters were measured, and the colony colours described after 3 wk according to the colour charts of Rayner (1970) .
9
Fungal Species Identification Protocol
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Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Microscopic photographs were captured using a Leica stereomicroscope (M205 FA) (Leica Microsystems, Wetzlar, Germany) (structure and size of stromata; structure and size of ectostromatic disc and ostioles). Microscopicl observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope (Nikon Corporation, Tokyo, Japan) equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. More than 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the colour charts of Rayner (1970) . Nomenclatural novelties and descriptions were deposited in MycoBank (www.mycobank.org; Crous et al. 2004) .
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