Chromatin immunoprecipitation on purified Treg cells was performed with Agarose ChIP Kit (Pierce) and anti-RBPJ (Cell signaling), anti-Notch1 (biolegend), anti- Foxp3 (MBL), anti-Histone H3 trimethylated on Lysine 4 (H3K4me3) (abcam), anti-dimethylated on Lysine 27 (H3K27me2) (Millipore) and respective isotype control antibodies. Purified DNA was subjected to real-time PCR with primers flanking RBPJ binding site at Ifng CNS-22 or with primers flanking Foxp3 binding sites on Pde3b and Ikzf2 as previously described 40 37.
Anti histone h3 trimethylated on lysine 4 h3k4me3
Manufactured by Abcam
Anti-Histone H3 trimethylated on Lysine 4 (H3K4me3) is an antibody that specifically recognizes the trimethylation of lysine 4 on histone H3. This post-translational modification is associated with active transcription.
Automatically generated - may contain errors
2 protocols using anti histone h3 trimethylated on lysine 4 h3k4me3
1
ChIP-qPCR Analysis of T-reg Cells
2
ChIP-qPCR Analysis of T-reg Cells
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Chromatin immunoprecipitation on purified Treg cells was performed with Agarose ChIP Kit (Pierce) and anti-RBPJ (Cell signaling), anti-Notch1 (biolegend), anti- Foxp3 (MBL), anti-Histone H3 trimethylated on Lysine 4 (H3K4me3) (abcam), anti-dimethylated on Lysine 27 (H3K27me2) (Millipore) and respective isotype control antibodies. Purified DNA was subjected to real-time PCR with primers flanking RBPJ binding site at Ifng CNS-22 or with primers flanking Foxp3 binding sites on Pde3b and Ikzf2 as previously described 40 37.
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