C maf sym0f1

Manufactured by Thermo Fisher Scientific

The C-MAF (sym0F1) is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a core function within the laboratory setting. The product specifications and details are available upon request.

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C-Maf (2 citations)

2 protocols using c maf sym0f1

Profiling Peripheral Blood Mononuclear Cells

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Fresh peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Ficoll-Paque™ PLUS (GE Healthcare Bioscience) and stained for cell surface antigens employing the following fluorescently labelled monoclonal and isotype control antibodies: CD45RA (HI100), CD3 (UCHT1, OKT3), CD4 (OKT4), CD8α (HIT8a, RDA-T8), CCR9 (L053E8), CCR6 (G034E3), CCR7 (G043H7), β1 (TS2/16), β7 (FIB504) (BioLegend), α4 (9F10) (BD), CXCR3 (49801) (R&D). Dead cells were stained with the 7-aminoactinomycin D (7-AAD) viability staining solution (BioLegend). In some experiments, intracellular staining was performed with antibodies against RORγt (AFKJS-9), T-BET (eBio4B10), and C-MAF (sym0F1) (eBioscience) using the Cytofix or Cytoperm™ Kit (Becton Dickinson). Mouse or rat serum (eBioscience) was used for blocking. Intracellular FOXP3 was stained using the PE anti-human FOXP3 staining kit (eBioscience). Cells were analysed or sorted by BD FACSCanto II or FACSAria II.

Kadowaki A., Saga R., Lin Y., Sato W, & Yamamura T. (2019). Gut microbiota-dependent CCR9+CD4+ T cells are altered in secondary progressive multiple sclerosis. Brain, 142(4), 916-931.

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Detailed Flow Cytometry Antibody Panel

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The following antibodies were used for flow cytometry. For mouse cells, CD4 (RM4-5; eBioscience), CD8a (53-6.7; BD Biosciences), CD90.2 (53-2.1, eBioscience), PD-1 (29F.1A12; BioLegend), CTLA-4 (UC10-4B9, eBioscience), CD25 (PC61; BD Biosciences), CD69 (H1.2F3; BD Biosciences), Foxp3 (FJK-16s; eBioscience), and c-Maf (sym0F1, eBioscience) were used. For human cells, CD4 (OKT4; BioLegend), CXCR4 (12G5; eBioscience), CCR7 (G043H7; BioLegend), CD127 (A019D5; BioLegend), IFN-γ (4S.B3; eBioscience), Foxp3 (236A/E7; eBioscience) CD8 (PRA-T8, BD Biosciences), CTLA4 (BNI3; BioLegend), TIGIT (MBSA43; eBioscience), CD45 (HI30, BD Biosciences), and PD-1 (MIH4; eBioscience) were used.

Seki A, & Rutz S. (2018). Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. The Journal of Experimental Medicine, 215(3), 985-997.

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