Anti cyclin e antibodies

Antibodies against CDK2, CDK6, cyclin D1, cyclin B, myc, HA, and Flag were purchased from MBL (Nagoya, Japan). Rabbit anti-Cullin1, anti-Skp1, and anti-cyclin E antibodies were from Santa Cruz (Dallas, TX, USA). Mouse anti-Skp1 and anti-Cullin1 antibodies were from Invitrogen (Carlsbad, CA, USA). Anti-Fbw7 antibody was from Sigma. Anti-Rbx1 antibody was from Cell Signaling Technology (Beverly, MA, USA). Anti-MAGE-C2 antibodies have been described previously (25). Antibodies specific for GAPDH, GFP and GST were from ProteinTech (Chicago, Illinois, USA). Mouse or rabbit IgG (Sigma) was used as control.

High-purity grade (≥98%) caffeine and crystal violet were purchased from Aladdin Bio-Chem Technology, Co., Ltd. (Shanghai, China). NADP, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and a NADP/NADPH Quantification Kit were obtained from Sigma-Aldrich (St. Louis, MO, United States). D-Glucose 6-phosphate, recombinant G6PDH protein, and the G6PDH Activity Colorimetric Assay Kit were purchased from BioVision Incorporated (Milpitas, CA, United States). Reactive Oxygen Species Detection Reagents and DMEM were purchased from Thermo Fisher Biochemical Products (Beijing) Co., Ltd. FBS and a mixed P/S were purchased from Biological Industries (Kibbutz Beit Haemek, Israel) and Solarbio (Beijing, China), respectively. Primary antibodies against G6PDH, NOX4, NOX2, SOD2, and Cyclin D1 were obtained from Abcam (Cambridge, MA, United States). Antibodies against STAT3, p-STAT3, Ki67, and β-actin were purchased from Cell Signaling Technology (Beverly, MA, United States). Anti-Catalase, anti-p47-phox, and anti-Cyclin E antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Anti-β-tubulin and horseradish peroxidase-conjugated secondary antibodies were purchased from Sino Biological, Inc. (Beijing, China) and R&D Systems (Minneapolis, MN, United States), respectively.