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Superdex 200 increase 10 300 or 5 150 column

Manufactured by Cytiva
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The Superdex 200 Increase 10/300 or 5/150 column is a size-exclusion chromatography column designed for the analysis and separation of proteins, peptides, and other biomolecules. It is characterized by a high-performance porous matrix that allows for efficient separation based on molecular size.

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Lab products found in correlation

Astra software package, by Wyatt Technology (2 mentions) Optilab t rex refractometer, by Wyatt Technology (2 mentions) Minidawn treos mals detector, by Wyatt Technology (2 mentions) Agilent 1260 infinity 2, by Agilent Technologies (2 mentions)

Close spelling variants of this product

Superdex 200 5/150 Increase column Superdex 200 increase 10/300 Superdex 200 5/150 column Superdex 200 10/300 Superdex 200 Increase Superdex 200 column Superdex 200

2 protocols using superdex 200 increase 10 300 or 5 150 column

1

SEC Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experiments were performed on an Agilent 1260 Infinity II HPLC system equipped with a Superdex 200 Increase 10/300 or 5/150 column (Cytiva), a miniDAWN TREOS MALS detector, and an Optilab T-rEX refractometer (Wyatt Technology Corp.). The column was equilibrated in SEC buffer and 40–150 µg of protein were applied after 0.1 µm filtration. Data were processed with the Astra software package (Wyatt Technology Corp.).
Borgert S.R., Henke S., Witzgall F., Schmelz S., zur Lage S., Hotop S.K., Stephen S., Lübken D., Krüger J., Gomez N.O., van Ham M., Jänsch L., Kalesse M., Pich A., Brönstrup M., Häussler S, & Blankenfeldt W. (2022). Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa. Nature Communications, 13, 7402.
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2

SEC Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experiments were performed on an Agilent 1260 Infinity II HPLC system equipped with a Superdex 200 Increase 10/300 or 5/150 column (Cytiva), a miniDAWN TREOS MALS detector, and an Optilab T-rEX refractometer (Wyatt Technology Corp.). The column was equilibrated in SEC buffer and 40–150 µg of protein were applied after 0.1 µm filtration. Data were processed with the Astra software package (Wyatt Technology Corp.).
Borgert S.R., Henke S., Witzgall F., Schmelz S., zur Lage S., Hotop S.K., Stephen S., Lübken D., Krüger J., Gomez N.O., van Ham M., Jänsch L., Kalesse M., Pich A., Brönstrup M., Häussler S, & Blankenfeldt W. (2022). Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa. Nature Communications, 13, 7402.
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