Zearalenone

Manufactured by Pribolab

Sourced in China

Zearalenone is a mycotoxin produced by certain fungi. It is used as a laboratory standard for quantitative analysis and research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Glycerol gel loading buffer, by Sangon (1 mentions) Millipore water purification system, by Merck Group (1 mentions) Trisodium citrate, by Sinopharm (1 mentions) Mgcl2, by Sinopharm (1 mentions) Ochratoxin b, by Pribolab (1 mentions) Aflatoxin b1, by Pribolab (1 mentions) Kanamycin, by Solarbio (1 mentions) T4 dna ligase, by Takara Bio (1 mentions) Methanol, by ANPEL (1 mentions) Acetonitrile, by ANPEL (1 mentions) Deoxynivalenol, by Pribolab (1 mentions) Infinite m200 pro, by Tecan (1 mentions)

4 protocols using zearalenone

Optimized Protocols for Mycotoxin Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

All DNA sequences (shown in Table S1) were synthesized by Sangon Biotech. Co., Ltd. (Shanghai, China) and purified using high-performance liquid chromatography (HPLC). Tris (2-carboxyethyl), Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), 6× Glycerol Gel Loading Buffer, and 10× TM buffer (80 mM MgSO₄; pH 7.4) were also obtained from Sangon Biotech. Co., Ltd. (Shanghai, China). Chloroauric acid (HAuCl₄), NaCl, MgCl₂, Trisodium citrate, and H₂O₂ were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hemin, 2-methylimidazole, 3,3′,5,5′-tetramethylbenzidine (TMB), Tween 20, and Tris-HCl buffer (pH 7.4) were provided by Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The AFB1 ELISA kit was bought from BEOSEN Food Safety Technology Co., Ltd. (Wuxi, China). Aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZEN), fumonisin B1 (FB1), aflatoxin B2 (AFB2), and ochratoxin (OTA) were obtained from Pribolab Co., Ltd. (Qingdao, China). Ultrapure water (18.2 MΩ·cm) was prepared through the Millipore water purification system.

Wang W., Li X., Zeng K., Lu Y., Jia B., Lv J., Wu C., Wang X., Zhang X, & Zhang Z. (2024). Improved Catalytic Activity of Spherical Nucleic Acid Enzymes by Hybridization Chain Reaction and Its Application for Sensitive Analysis of Aflatoxin B1. Sensors (Basel, Switzerland), 24(7), 2325.

+ Open protocol

+ Expand

Multiplexed Detection of Mycotoxins

Check if the same lab product or an alternative is used in the 5 most similar protocols

All DNA strands listed in Table 1 were synthesized and purified by Sangon Biotech Inc. (Shanghai, China). Aflatoxin B1 (AFB1), ochratoxin A (OTA), ochratoxin B (OTB), fumonisin B1 (FB1), fumonisin B2 (FB2), and zearalenone (ZAE) were purchased from Pribolab (Qingdao, China). Beer and corn flour were bought from a local supermarket. Assay buffer was 10 mM Tris-HCl (pH 7.5) solution containing 50 mM NaCl, 50 mM MgCl₂, and 0.1% Tween20. Black 96-wells microplates were purchased from Thermo Fisher Scientific Inc. (Waltham, USA). All reagents used for experiments were analytical reagents. Solutions were prepared with ultrapure water (>18.2 MΩ·cm) from a Purelab Ultra Genetic Elga Labwater system (High Wycombe, UK).

Wang C., Yu H, & Zhao Q. (2022). A Simple Structure-Switch Aptasensor Using Label-Free Aptamer for Fluorescence Detection of Aflatoxin B1. Molecules, 27(13), 4257.

+ Open protocol

+ Expand

Cloning and Expression of Zearalenone-Degrading Enzyme

Check if the same lab product or an alternative is used in the 5 most similar protocols

E. coli JM109 was used as the cloning host strain. The plasmid pET28a (+) and E. coli BL21 (DE3) were used for gene expression. Luria–Bertani (LB) medium was prepared to culture the strains. Restriction enzymes (BamHI and HindIII), Ex Taq DNA polymerase, T4 DNA ligase, and other related enzymes were obtained from Takara (Dalian, China). Isopropyl-β-D-1-thiogalactopyranosid (IPTG) and kanamycin were purchased from Solarbio (Beijing, China). Zearalenone was bought from Pribolab (Qingdao, China) and dissolved in acetonitrile as a standard stock solution (1 mg/mL). acetonitrile and methanol of HPLC grade were purchased from ANPEL laboratory Technologies (Shanghai, China).

Zhang Y., Liu X., Zhang Y., Zhang X, & Huang H. (2022). Cloning and Characterization of Three Novel Enzymes Responsible for the Detoxification of Zearalenone. Toxins, 14(2), 82.

+ Open protocol

+ Expand

Mycotoxin Detection Using QD-Nanobody

Check if the same lab product or an alternative is used in the 5 most similar protocols

Restriction enzymes and PrimeSTAR^® HS DNA polymerase were supplied by Takara Biomedical Tech. Co. Ltd. (Beijing, China). The primers listed in Table S1 were synthesized by Sangon Biotech (Shanghai, China). Gluconic acid, N, N-dicyclohexyl carbodiimide (DCC), N, N-dimethyl formamide (DMF), and N-hydroxysuccinimide (NHS) were obtained from Aladdin (Shanghai, China). Standards of mycotoxins, including aflatoxin B₁ (AFB₁), deoxynivalenol (DON), fumonisin B₁ (FB₁), OTA, ochratoxin B (OTB), ochratoxin C (OTC), and zearalenone (ZEN) were purchased from Pribolab (Qingdao, China). The ZnCdSe/ZnS QDs modified with an amino group (RQD-NH₂, λ_em=614 nm) were supplied by Jiayuan Tech. (Wuhan, China). The recombinant vectors, pET25b-Nb28 encoding the Nb gene against OTA and pGEM-T-SGFP encoding the SGFP gene, were previously prepared in our laboratory. The remaining inorganic chemicals and organic solvents were of analytical grade or purity.
A spectral scanning multimode reader was used to scan the fluorescence spectra and UV–vis spectra (Infinite M200 Pro, Tecan, Switzerland). A PerkinElmer Frontier infrared spectrometer was used to analyze the Fourier transform infrared spectroscopy (FTIR) spectra (PerkinElmer, Waltham, MA, USA).

Su B., Zhang Z., Sun Z., Tang Z., Xie X., Chen Q., Cao H., Yu X., Xu Y., Liu X, & Hammock B.D. (2021). Fluonanobody-Based Nanosensor Via Fluorescence Resonance Energy Transfer for Ultrasensitive Detection of Ochratoxin A. Journal of hazardous materials, 422, 126838.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!