Automatic immunostaining device

Manufactured by Leica

The Leica automatic immunostaining device is a laboratory equipment designed for the automated immunohistochemical staining of tissue samples. It performs the necessary steps for the staining process, including antigen retrieval, blocking, primary antibody incubation, and detection. The device is intended to provide consistent and reproducible results, while reducing the time and labor required for manual staining procedures.

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Lab products found in correlation

Desmin, by Gene Tech (2 mentions) Calretinin, by ZSGB-BIO (2 mentions) α inhibin, by Beijing Strong Biotechnologies (2 mentions)

2 protocols using automatic immunostaining device

Immunohistochemical Profiling of Tumor Samples

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Collected tumours or normal tissue after removal of paraffin as the experimental subject. Consecutive 4-μm thick unstained sections for immunohistochemical staining, which was performed using the Leica automatic immunostaining device (Leica Microsystems, Inc.). Primary antibodies against CD10 (1:100; no. 563871; DAKO; DK), α-inhibin (1:100; no. GT230202; CHN), SMA (1:50; no. MAB-0980; MXB; CHN), desmin (1:300; no. GT225202; Gene tech; CHN), TFE3 (1:100; no. ZA-0657; ZSGB-BIO; CHN), calretinin (1:100; no. ZM-0063; ZSGB-BIO; CHN), WT-1 (1:100; no. ZM-0269; ZSGB-BIO; CHN), and EMA (1:300; no. GM061302; Gene tech; CHN). Appropriate positive and negative controls were simultaneously stained to validate the staining method.

Zhao L., Zhou Y., Liu Y., Luo Q., Jiang Q., Wang H, & Wang N. (2023). TFE3 is a Novel Biomarker of Ovarian Sclerosing Stromal Tumours. Research Square.

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Immunohistochemical Staining of Tumor Samples

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As the experimental subjects, tumors or normal tissues were collected after paraffin removal. Consecutive 4-μm-thick unstained sections were used for immunohistochemical staining, which was performed using the Leica automatic immunostaining device (Leica Microsystems, Inc.). Primary antibodies against CD10 (1:100; no. 563871; DAKO; DK), α-inhibin (1:100; no. GT230202; CHN), SMA (1:50; no. MAB-0980; MXB; CHN), desmin (1:300; no. GT225202; Gene tech; CHN), TFE3 (1:100; no. ZA-0657; ZSGB-BIO; CHN), calretinin (1:100; no. ZM-0063; ZSGB-BIO; CHN), WT-1 (1:100; no. ZM-0269; ZSGB-BIO; CHN), and EMA (1:300; no. GM061302; Gene tech; CHN). Appropriate positive and negative controls were simultaneously stained to validate the staining method.

Zhao L., Yang Z., Zhou Y., Liu Y., Luo Q., Jiang Q., Wang H, & Wang N. (2023). TFE3 nuclear expression as a novel biomarker of ovarian sclerosing stromal tumors and associated with its histological morphology. Journal of Ovarian Research, 16, 152.

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