The patient’s whole blood was collected at the time of enrollment. Blood samples were centrifuged, and separated serum were stored at −80ºC until analysis of enzyme-linked immunosorbent assay (ELISA). We measured serum markers related to preeclampsia,22 (link) such as serum soluble fms-like tyrosine kinase-1 (sFlt-1; MBS262972-96T, MyBiosource, San Diego, CA, USA), endoglin (DNDG00, R&D Systems, Minneapolis, MN, USA), and placental growth factor (PlGF; LS-F1231, LSBio, Seattle, WA, USA). Biomarkers of OSA were evaluated, including interleukin (IL)-6 (D6050, R&D Systems), myeloid-related protein (MRP)8/14 (439707, Biolegend, San Diego, CA, USA), and tumor necrosis factor (TNF)-α (DTA00D, R&D Systems).23 (link)
Dndg00
Manufactured by R&D Systems
Sourced in United States
DNDG00 is a laboratory equipment designed for general research purposes. It serves as a tool for conducting experiments and analyses in a controlled laboratory environment. The core function of DNDG00 is to provide a standardized and reliable platform for data collection and analysis. Further details on the intended use or specific applications of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Glomax multi detection system, by Promega (2 mentions)
Dta00d, by R&D Systems (1 mentions)
D6050, by R&D Systems (1 mentions)
Dve00, by R&D Systems (1 mentions)
Quantikine human endoglin cd105, by R&D Systems (1 mentions)
Quantikine human vegf, by R&D Systems (1 mentions)
Human bmp4 quantikine elisa kit, by R&D Systems (1 mentions)
Elecsys sflt 1, by Roche (1 mentions)
3 protocols using dndg00
1
Preeclampsia and OSA Biomarker Evaluation
2
Quantifying sEng and VEGF in RC and CC
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Blood samples were collected from 37 RC and 81 CC patients at time 0 (immediately before surgery) and at different time points (3, 6, and 9 months) after resection of the tumor sample. sEng and human VEGF concentrations in plasma were measured and quantified by ELISA, according to the manufacturer’s protocol by Quantikine Human Endoglin/CD105 and Quantikine Human VEGF, respectively (DNDG00 and DVE00; R&D Systems). All immunoassays were measured with a GloMax multidetection system (Promega).
3
Quantification of Angiogenic Factors
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Human BMP4 from HUVECs culture supernatants or from human sera was measured with the Human BMP4 Quantikine ELISA Kit (DBP400; R&D Systems). To measure BMP4 in mouse plasma the Mouse BMP4 ELISA Kit (LSF13543; sensitivity range 15.6–1000 pg/mL; LSBio) was used. Concentration of human sEng in human or mouse sera was determined by ELISA (DNDG00; sensitivity range 0.2–10 ng/mL; R&D Systems), whereas concentration of sFlt1 in human sera was measured using an electrochemiluminescence immunoassay (Elecsys® sFlt-1, Roche). All immunoassays were performed following the manufacturer’s instructions and measured in a GloMax multidetection system (Promega).
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