Ld efluor780

Manufactured by Thermo Fisher Scientific

Sourced in United States

The LD eFluor780 is a fluorescent dye that can be used for cell staining and flow cytometry applications. It is a far-red fluorescent dye that binds to intracellular proteins, allowing for the identification and analysis of cells. The LD eFluor780 dye has an excitation wavelength of 780 nm and an emission wavelength of 810 nm.

Automatically generated - may contain errors

Lab products found in correlation

Cd8 v500, by BD (1 mentions) Granzyme b fitc, by Thermo Fisher Scientific (1 mentions) Cd45ra bv605, by BD (1 mentions) Tnfα efluor 450, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Cd8 percp cy5, by Thermo Fisher Scientific (1 mentions) Pd 1 pe, by BD (1 mentions) Cd3 buv395, by BD (1 mentions) Granzyme b apc, by BioLegend (1 mentions) F4 80 bv510, by BioLegend (1 mentions) Ifn γ bv421, by BD (1 mentions) Cd11b pe cy7, by BioLegend (1 mentions) Cd4 buv737, by BD (1 mentions)

2 protocols using ld efluor780

Multiparametric Flow Cytometry Profiling

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All antibodies are from BioLegend, USA unless otherwise noted: surface staining: CCR7 (CD197)-PerCP-Cy5.5; clone: G043H7, CD4-A700; clone: OKT4, LD eFluor780 (eBioscience, USA), CD8-V500; clone: RPA-T8 (BD Biosciences, USA), CD45RA-BV605; clone: HI100. Intracellular staining: granzyme B-FITC; clone: GB11, IL-2-PE; clone: MQ1-17H12, CD137 (4-1BB)-PE-Cy7; clone: 4B4-1, CD154 (CD40L)-A647; clone: 24–31, TNFα-eFluor450; clone: MAb11 (eBioscience, USA), IFNg-BV650; clone: 4S.B3, CD3-BV785; clone: OKT3. Fixable Viability Dye eFluor 780(eBioscience, USA) was used for live/dead discrimination.

Anft M., Blazquez-Navarro A., Stervbo U., Skrzypczyk S., Witzke O., Wirth R., Choi M., Hugo C., Reinke P., Meister T.L., Steinmann E., Pfaender S., Schenker P., Viebahn R., Westhoff T.H, & Babel N. (2021). Detection of pre-existing SARS-CoV-2-reactive T cells in unexposed renal transplant patients. Journal of Nephrology, 34(4), 1025-1037.

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Comprehensive Immune Cell Analysis

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The following antibodies were used for flow cytometry analysis: CD45-FITC (Cat. # 553079; BD Biosciences), CD3-BUV395 (Cat. # 563565; BD Biosciences), CD4-BUV737 (Cat. # 612761; BD Biosciences), CD8-Percp-Cy5.5 (Cat. # 45-0081-82; eBioscience), CD44-BV711 (Cat. # 103057; Biolegend), CD335-PE/Dazzle594 (Cat. #137630; Biolegend), PD-1-PE (Cat. # 551892; BD Biosciences), Ki67*-BV605 (Cat. # 652413; Biolegend), Granzyme B*-APC (Cat. # 366408; Biolegend), IFN-γ*-BV421 (Cat. # 563376; BD Biosciences), CD11b-PE-Cy7 (Cat. # 101216; Biolegend), F4/80-BV510 (Cat. # 123135; Biolegend), CD206-AF700 (Cat. # 141734; Biolegend), I-A/I-E-BV786 (Cat. # 743875; BD Biosciences), and L/D-efluor780 (Cat. # 65-0865-18; eBioscience).

Zhang Y., Gabere M., Taylor M.A., Simoes C.C., Dumbauld C., Barro O., Tesfay M.Z., Graham A.L., Ferdous K.U., Savenka A.V., Chamcheu J.C., Washam C.L., Alkam D., Gies A., Byrum S.D., Conti M., Post S.R., Kelly T., Borad M.J., Cannon M.J., Basnakian A, & Nagalo B.M. (2022). Repurposing live attenuated trivalent MMR vaccine as cost-effective cancer immunotherapy. Frontiers in Oncology, 12, 1042250.

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