T2A, E3330, CRT0044876, etoposide (Eto), mitomycin C (MMC), methyl methanesulfonate (MMS), hydroxyurea (HU), camptothecin (Cpt), doxycycline (DOX), dimethyl sulfoxide (DMSO), and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Cell culture media, fetal bovine serum, and trypsin were obtained from Thermo Scientific HyClone (Logan, UT, USA). The LightShift Chemiluminescence Electrophoretic Mobility-Shift Assay (EMSA) kit, SuperSignal West Pico chemiluminescence reagents, and horseradish peroxidase-conjugated antimouse or antirabbit IgG antibodies were purchased from Thermo Fisher Scientific, Waltham, MA, USA.
Lightshift chemiluminescence electrophoretic mobility shift assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The LightShift Chemiluminescence EMSA kit is a laboratory tool used to study protein-DNA interactions. It allows researchers to detect and analyze the binding of transcription factors or other DNA-binding proteins to specific DNA sequences. The kit provides the necessary components, including labeled DNA probes and reagents, to perform electrophoretic mobility shift assays, which can be used to identify and characterize these protein-DNA interactions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Trypsin, by Thermo Fisher Scientific (3 mentions)
Cell culture media, by Thermo Fisher Scientific (3 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Horseradish peroxidase conjugated anti mouse or anti rabbit igg antibodies, by Thermo Fisher Scientific (1 mentions)
E3330, by Merck Group (1 mentions)
Crt0044876, by Merck Group (1 mentions)
Supersignal west pico chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions)
Ne per nuclear protein cytoplasmic protein extraction kit, by Thermo Fisher Scientific (1 mentions)
Protein assay reagent, by Bio-Rad (1 mentions)
Trypsin, by GE Healthcare (1 mentions)
Restriction endonuclease, by Promega (1 mentions)
Dulbecco s modified eagle s medium dmem, by GE Healthcare (1 mentions)
Dual luciferase assay system kit, by Promega (1 mentions)
Prl tk vector, by Promega (1 mentions)
Gossypol, by Merck Group (1 mentions)
T4 polynucleotide kinase, by Promega (1 mentions)
Myricetin, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
T4 ligase, by Promega (1 mentions)
3 protocols using lightshift chemiluminescence electrophoretic mobility shift assay kit
1
Cellular Stress Response Assessment
2
Analyzing ATG16L1 gene promoter interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nuclear extracts of the HEK-293 and H9c2 cells were prepared with an NE-PER® Nuclear Protein/Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Inc.). The protein concentration of the nuclear extract was determined using Bio-Rad Protein Assay Reagent and stored at –80°C until use. Biotinylated double-stranded oligonucleotides (30 bp) were used that contained SNP sites as probes. A LightShift® chemiluminescence electrophoretic mobility shift assay (EMSA) kit (Thermo Fisher Scientific, Inc.) was used for the DNA-protein binding reaction to explore the interaction between the DNA fragment of ATG16L1 gene promoter and the nucleoprotein. Unfortunately, due to the limitations of EMSA experimental technology, it is impossible to determine whether the protein directly interacts with the polymorphic sites on the promoter or indirectly affects the transcription process of the gene.
3
Molecular Mechanisms of Natural Compound Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gossypol and myricetin were purchased from Sigma-Aldrich (St Louis, MO, USA), dissolved in 100% dimethyl sulfoxide, and stored as 10 mM stocks at −20°C. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), and trypsin were obtained from GE Healthcare Life Sciences (Logan, UT, USA). A LightShift chemiluminescence electrophoretic mobility shift assay (EMSA) kit was purchased from Thermo Fisher Scientific (Rockford, IL, USA). The luciferase-harboring vector (pGL3)-control vector, thymidine kinase-driven Renilla luciferase (pRL-TK) vector, Dual-Luciferase Assay System kit, T4 polynucleotide kinase, T4 ligase, restriction endonucleases, and high-fidelity Pyrococcus furiosis DNA polymerase were from Promega (Madison, WI, USA). A Cell Counting Kit-8 (CCK-8) was purchased from Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!