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Novolin 30r penfill

Manufactured by Novo Nordisk
Sourced in Denmark

Novolin® 30R Penfill® is a pre-filled insulin pen device used for the subcutaneous administration of a 30% regular insulin and 70% isophane insulin suspension. It is designed to provide a combined insulin action profile for the management of diabetes.

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2 protocols using novolin 30r penfill

1

Glucose and Insulin Tolerance Tests in Mice

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GTT was carried out on mice by intraperitoneal injection with 1 g/kg body weight of D-glucose after fasting for 16 h. For ITT, mice were injected intraperitoneally with regular human insulin (Novolin® 30R Penfill®, Novo Nordisk, Bagsværd, Denmark) at a dose of 0.75 U/kg body weight. Blood glucose levels were measured from the tail vein at 0, 15, 30, 60, and 120 min after injection using a blood glucose meter (ACCU-CHEK Instant, Roche, Basel, Switzerland).
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2

Glucose, Pyruvate, and Insulin Tolerance Tests

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Glucose tolerance tests and pyruvate tolerance tests were carried out on mice by i.p. injection with 1 or 2 g/kg body weight of D-glucose or pyruvate (Sigma-Aldrich) after a 16 h fast. The insulin tolerance tests were also performed on 6 h fasted mice with the i.p. injection of regular human insulin (Novolin® 30R Penfill®, Novo Nordisk) at a dose of 0.75 U/kg body weight. Blood glucose levels were detected from the tail vein before injection and at 15, 30, 60 and 120 min after injection using a portable blood glucose meter (FreeStyle Optium Neo, Abbott).
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