Milliplex map cytokine chemokine panel and immunoassay

Manufactured by Merck Group

Sourced in United States

The Milliplex MAP Cytokine/Chemokine Panel and Immunoassay is a multiplex assay used for the simultaneous quantitative detection of multiple analytes in a single sample. It utilizes Luminex xMAP technology to measure cytokines, chemokines, and other proteins. The assay provides a comprehensive analysis of the target analytes in a time-efficient and cost-effective manner.

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Milliplex MAP Cytokine Immunoassay MILLIPLEX MAP Milliplex immunoassay Milliplex panels

2 protocols using milliplex map cytokine chemokine panel and immunoassay

Inflammatory Cytokine and Cardiovascular Marker Assays

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The serum cytokines can be elevated/deranged as a result of a systemic or local inflammatory response following nanoparticle exposure, contributing to the changes in vascular tissue contractility. Thus, selected serum cytokines and chemokines (IL6, IL10, TNFα, MCP1, PAI1, VEGF, INFγ, and IL1β) were assessed using Milliplex MAP Cytokine/Chemokine Panel and Immunoassay (EMD Millipore MA, USA). Serum cardiovascular disease markers (PAI1 and vWF) were assessed using Milliplex MAP Rat Cardiovascular Panel and Immunoassay (EMD Millipore MA, USA) from both maternal and fetal serum according to the manufacturer’s directions. Assays were run using a Luminex 100/200 (Luminex, Austin, TX) and results reported using Luminex xPONENT® software versions 2.3/3.1.

Vidanapathirana A.K., Thompson L.C., Mann E.E., Odom J.T., Holland N.A., Sumner S.J., Han L., Lewin A.H., Fennell T.R., Brown J.M, & Wingard C.J. (2014). PVP formulated Fullerene (C60) increases Rho-kinase dependent Vascular Tissue Contractility in Pregnant Sprague Dawley Rats. Reproductive toxicology (Elmsford, N.Y.), 49, 86-100.

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Maternal-Fetal Serum Cytokine Analysis

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The targets for maternal and fetal serum cytokine analysis were selected based on the reported cytokine targets in previous MWCNT exposure studies [4 (link),33 (link),34 (link)]. The selected serum cytokines and chemokines (IL6, IL10, TNFα, MCP1, VEGF, INFγ, and IL1β) were assessed using Milliplex MAP Cytokine/Chemokine Panel and Immunoassay (EMD Millipore MA, USA) according to the manufacturer’s directions. Assays were run using Luminex 100/200 (Luminex, Austin, TX) and results reported using Luminex xPONENT^® software versions 2.3/3.1.

Vidanapathirana A., Thompson L., Odom J., Holland N., Sumner S., Fennell T., Brown J, & Wingard C. (2014). Vascular Tissue Contractility Changes Following Late Gestational Exposure to Multi-Walled Carbon Nanotubes or their Dispersing Vehicle in Sprague Dawley Rats. Journal of nanomedicine & nanotechnology, 5(3), 201.

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