Vero E6 cells were infected with LASV VLPVs at multiplicity of infection (MOI) of 1. On day 2 (D2), cells were fixed with 4% paraformaldehyde, permeabilized, stained with antibodies against LASV GPC and/or against fibritin, calnexin (clone 1D4), syntaxin-6 (LifeSpan BioSciences) and analyzed with a Zeiss AX10 microscope. LASV GP expression was also measured by flow cytometry (FACScalibur, BD Bioscience, San Diego, CA) using LASV GP2 or fibritin antibody and Texas Red-conjugated anti-rabbit IgG. Apoptosis was measured by TUNEL assay using the ApopTag Red Apoptosis Detection Kit.
Apoptag red apoptosis detection kit
Manufactured by BD
The ApopTag Red Apoptosis Detection Kit is a laboratory equipment product that is used to detect and analyze apoptosis, a programmed cell death process, in cell samples. The kit provides the necessary reagents and protocols to perform this analysis.
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2 protocols using apoptag red apoptosis detection kit
1
Visualization of LASV VLPs in Vero E6 Cells
2
Visualization of LASV VLPs in Vero E6 Cells
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Vero E6 cells were infected with LASV VLPVs at multiplicity of infection (MOI) of 1. On day 2 (D2), cells were fixed with 4% paraformaldehyde, permeabilized, stained with antibodies against LASV GPC and/or against fibritin, calnexin (clone 1D4), syntaxin-6 (LifeSpan BioSciences) and analyzed with a Zeiss AX10 microscope. LASV GP expression was also measured by flow cytometry (FACScalibur, BD Bioscience, San Diego, CA) using LASV GP2 or fibritin antibody and Texas Red-conjugated anti-rabbit IgG. Apoptosis was measured by TUNEL assay using the ApopTag Red Apoptosis Detection Kit.
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