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Sp5 inverted confocal point scanning microscope

Manufactured by Leica
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Sourced in Germany

The SP5 is an inverted confocal point scanning microscope designed and manufactured by Leica. It is capable of performing high-resolution imaging and analysis of samples. The core function of the SP5 is to capture detailed microscopic images using a confocal scanning technique.

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Lab products found in correlation

Tcs spe 2 laser scanning confocal microscope, by Leica (1 mentions) Ti s microscope, by Nikon (1 mentions) Led4d120, by Thorlabs (1 mentions) Lf405 488 532 635 a 000, by IDEX Corporation (1 mentions) 5.5 scmos camera, by Oxford Instruments (1 mentions)

2 protocols using sp5 inverted confocal point scanning microscope

1

Laser Scanning Confocal Microscopy Protocol

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Images were detected on a Leica TCS SPE II laser scanning confocal microscope using 400 × magnification. Expansion microscopy was performed on a Leica SP5 inverted confocal point scanning microscope at the UW Biology Imaging Core. See Supplementary Methods for details.
Kaverina N.V., Eng D.G., Freedman B.S., Kutz J.N., Chozinski T.J., Vaughan J.C., Miner J.H., Pippin J.W, & Shankland S.J. (2019). Dual lineage tracing shows that glomerular parietal epithelial cells can transdifferentiate toward the adult podocyte fate. Kidney international, 96(3), 597-611.
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2

Epifluorescence and Confocal Microscopy

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Conventional epifluorescence microscopy was performed with an inverted Nikon Ti-S microscope fitted with a 4 × 0.2 NA air objective lens (Nikon, Melvill, NY, USA), a 10 × 0.25 NA air objective lens (Nikon), or a 20 × 0.45 NA air objective lens (Nikon). Illumination was achieved using a four-channel light emitting diode (LED4D120, Thorlabs, Newton, NJ, USA) with a multiband filter set LF405/488/532/635-A-000, Semrock, Rochester, NY, USA) and images were captured using a Zyla 5.5 sCMOS camera (Andor, Windsor, CT, USA). Confocal microscopy was performed on a Leica SP5 inverted confocal point scanning microscope at the UW Biology Imaging Core using a 10 × 0.4 NA air objective lens (Leica, Nussloch, Germany), 20 × 0.7 NA air objective (Leica), and a 63 × 1.2 NA water immersion objective (Leica).
Chozinski T.J., Mao C., Halpern A.R., Pippin J.W., Shankland S.J., Alpers C.E., Najafian B, & Vaughan J.C. (2018). Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy. Scientific Reports, 8, 10396.
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