Raw 264.7 murine macrophage cell line

Manufactured by Korean Cell Line Bank

The RAW 264.7 murine macrophage cell line is a widely used in vitro model derived from Mus musculus (mouse) macrophages. This cell line serves as a tool for researchers to study various aspects of macrophage biology, including cell signaling, immune response, and host-pathogen interactions.

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Lab products found in correlation

Fetal bovine serum (fbs), by Welgene (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Welgene (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Penicillin, by Cytiva (1 mentions) Streptomycin, by Cytiva (1 mentions) Penicillin streptomycin solution, by Cytiva (1 mentions) Streptomycin, by Welgene (1 mentions) L glutamine, by Welgene (1 mentions) Schisandrin a, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Penicillin, by Welgene (1 mentions) Raw264, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Versamax microplate reader, by Molecular Devices (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin g, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Versamax, by Molecular Devices (1 mentions) Mc3t3 e1 cell, by American Type Culture Collection (1 mentions)

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RAW 264.7 (104 citations)

13 protocols using raw 264.7 murine macrophage cell line

Murine Macrophage Cell Culture Protocol

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The RAW 264.7 murine macrophage cell line was purchased from the Korea Cell Line Bank (Seoul, Korea) and cultured in DMEM containing 10% fetal bovine serum, penicillin-streptomycin (100 units/mL) at 37°C with 5% CO₂.
Murine bone marrow derived macrophages (BMDMs) were isolated from femur of C57BL/6 mice. Cells in bone marrow were washed several times with cold phosphate buffered saline (PBS). The isolated cells were filtered through sieve mesh, centrifuged, and resuspended in DMEM (supplemented with 10% FBS, 100 units/mL penicillin-streptomycin). The cells were incubated at 2 × 10⁶ in Petri dish with 15% L929-conditioned medium in DMEM for 7 days to differentiate into macrophages. The culture medium was added at the 3rd day and replaced at the 6th day of incubation. After being differentiated, the cells were seeded in 24-well culture plates. In all experiments, cells were incubated with samples at various concentrations that was always added 1 h prior to LPS (1 μg/mL) treatment for the indicated time.

Han H.S., Jang E., Shin J.S., Inn K.S., Lee J.H., Park G., Jang Y.P, & Lee K.T. (2017). Kyungheechunggan-Tang-01, a New Herbal Medication, Suppresses LPS-Induced Inflammatory Responses through JAK/STAT Signaling Pathway in RAW 264.7 Macrophages. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 7383104.

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Cultivation of Murine Macrophages and Human Mast Cells

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The RAW 264.7 murine macrophage cell line and the human mast cell (HMC-1) line were purchased from the Korea Cell Line Bank (Seoul, Korea). Each cell line was grown in Dulbecco’s modified Eagle’s medium (DMEM, Welgene, Daegu, Korea) or Iscove’s Modified Dulbecco’s Medium (IMDM, Welgene, Daegu, Korea) supplemented with 10% heat-inactivated FBS (Welgene, Daegu, Korea) and 1% antibiotics (Ab, Welgene, Daegu, Korea) at 37°C and 5% CO₂.

Hong S.H., Ku J.M., Kim H.I., Kim T.Y., Seo H.S., Shin Y.C, & Ko S.G. (2019). Topical Application of KAJD Attenuates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis Symptoms Through Regulation of IgE and MAPK Pathways in BALB/C Mice and Several Immune Cell Types. Frontiers in Pharmacology, 10, 1097.

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Culturing Raw 264.7 Murine Macrophages

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Raw 264.7 murine macrophage cell line was purchased from Korean Cell Line Bank (Seoul, Korea) and was routinely cultured in Dulbecco’s modified Eagle medium (DMEM), appended with 10% (v/v) Fetal Bovine Serum and 1% (v/v) penicillin-streptomycin at 37°C in an incubator supplemented with 5% CO₂.

Shrestha A., Pun N.T, & Park P.H. (2018). ZFP36L1 and AUF1 Induction Contribute to the Suppression of Inflammatory Mediators Expression by Globular Adiponectin via Autophagy Induction in Macrophages. Biomolecules & Therapeutics, 26(5), 446-457.

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Culturing Murine Macrophage Cell Line

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The RAW 264.7 murine macrophage cell line was obtained from the Korea Cell Line Bank (Seoul, Korea). The cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Rockville, IL, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) and penicillin–streptomycin solution (100 U/mL penicillin and 100 µg/mL streptomycin; Hyclone Laboratories Inc., South Logan, UT, USA). The cells were grown in a 5% CO₂ humidified atmosphere at 37 °C.

Cho S.Y., Kim H.W., Lee M.K., Kim H.J., Kim J.B., Choe J.S., Lee Y.M, & Jang H.H. (2020). Antioxidant and Anti-Inflammatory Activities in Relation to the Flavonoids Composition of Pepper (Capsicum annuum L.). Antioxidants, 9(10), 986.

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Schisandrin A Modulates Macrophages

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The RAW 264.7 murine macrophage cell line was obtained from the Korea Cell Line Bank (Seoul, Korea) and maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v/v) fetal bovine serum, L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 U/ml) (all from WelGENE Inc., Daegu, Korea) at 37°C in a humidified atmosphere containing 5% CO₂ and 95% air. Schisandrin A was purchased from Sigma-Aldrich (cat. no. SML0054; Merck KGaA, Darmstadt, Germany), dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) and final concentrations were adjusted by dilution with complete culture medium. The DMSO concentration did not exceed 0.05% (i.e., a non-cytotoxic range). To stimulate the cells, the medium was replaced with fresh DMEM, and LPS (E. coli Serotype 055:B5; cat. no. L2880; Sigma-Aldrich; Merck KGaA) was added in the presence or absence of Schisandrin A for the indicated periods.

Kwon D.H., Cha H.J., Choi E.O., Leem S.H., Kim G.Y., Moon S.K., Chang Y.C., Yun S.J., Hwang H.J., Kim B.W., Kim W.J, & Choi Y.H. (2017). Schisandrin A suppresses lipopolysaccharide-induced inflammation and oxidative stress in RAW 264.7 macrophages by suppressing the NF-κB, MAPKs and PI3K/Akt pathways and activating Nrf2/HO-1 signaling. International Journal of Molecular Medicine, 41(1), 264-274.

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Culturing of Murine Macrophages and Human Cell Lines

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The RAW 264.7 murine macrophage cell line was purchased from the Korea Cell Line Bank (Seoul, South Korea). The RAW 264.7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, NY, USA), 100 U/mL of penicillin, and 100 μg/mL of streptomycin in 5% CO₂ at 37°C. HaCat cells (human keratinocyte cell line) and Hep3B cells were cultured in RPMI 1640 medium containing 10% (v/v) FBS and 1% penicillin-streptomycin (10,000 U and 10,000 μg/mL, respectively) in 5% CO₂ at 37°C.

Kim B., Kim J.E., Choi B.K, & Kim H.S. (2015). Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-κB-signaling Pathway. Biomolecules & Therapeutics, 23(1), 90-97.

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Evaluating FTM Cytotoxicity in RAW 264.7 Cells

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The RAW 264.7 murine macrophage cell line was purchased from the Korean Cell Line Bank (Seoul, Korea). Cells were cultured in DMEM containing 10% FBS and 1% P/S at 37˚C (5% CO₂, 95% humidity). To determine the effects of FTM on cellular viability, RAW 264.7 cells (5x10⁴ cells/well) were seeded into 96-well culture plates, and cultured in serum-free medium for 24 h in the presence or absence of FTM (12.5, 25, 50 and 100 µg/ml). Then, 10 µl CCK-8 reagent was added to each well and the plates were incubated for an additional 2 h. Cell viability was determined by absorbance measurement at a wavelength of 450 nm (VersaMax microplate reader; Molecular Devices, LLC). The absorbance values are expressed as a percentage of the non-treated cells, and a value of <90% was considered to indicate cytotoxicity. Serum-free medium was used to eliminate serum-associated interference.

Kim J.H., Kim M., Hong S., Kwon B., Song M.W., Song K., Kim E.Y., Jung H.S, & Sohn Y. (2021). Anti-inflammatory effects of Fritillaria thunbergii Miquel extracts in LPS-stimulated murine macrophage RAW 264.7 cells. Experimental and Therapeutic Medicine, 21(5), 429.

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Macrophage Response to TECA Exposure

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The RAW 264.7 murine macrophage cell line was obtained from the Korea Cell Line Bank (Seoul, Korea). These cells were grown at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM) medium supplemented with 10% FBS, penicillin (100 units/mL) and streptomycin sulfate (100 μg/mL) in humidified atmosphere of 5% CO₂. Cells were incubated with TECA at various concentrations (1, 2, or 5 μg/mL) or positive chemicals and then stimulated with LPS 1 μg/mL for the indicated time in figure legends. Various concentrations of TECA dissolved in ethanol were added together with LPS. The final concentration of ethanol used was less than 0.05%. Cells were treated with 0.05% ethanol as vehicle control.

Park J.H., Choi J.Y., Son D.J., Park E.K., Song M.J., Hellström M, & Hong J.T. (2017). Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model. International Journal of Molecular Sciences, 18(4), 738.

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RAW 264.7 Macrophage Cell Culture

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RAW 264.7 murine macrophage cell line was purchased from the Korean Cell Line Bank (Seoul, South Korea). The cells were grown in culture flasks containing Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 IU/mL penicillin G and 100 mg/mL streptomycin; Gibco BRL, Grand Island, NY, USA). Cell cultures were cultured at 37 °C in a humidified atmosphere of 5% CO₂.

Youn S.H., Kwon J.H., Yin J., Tam L.T., Ahn H.S., Myung S.C, & Lee M.W. (2017). Anti-Inflammatory and Anti-Urolithiasis Effects of Polyphenolic Compounds from Quercus gilva Blume. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(7), 1121.

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Carnosol Modulates Murine Macrophage Response

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The RAW 264.7 murine macrophage cell line was obtained from the Korea Cell Line Bank (Seoul, Korea). These cells were grown at 37°C in DMEM medium supplemented with 10% FBS, penicillin (100 units/ml) and streptomycin sulfate (100 μg/mL) in a humidified atmosphere of 5% CO₂. Cells were incubated with carnosol at various concentrations (1, 2, or 5 μM) or positive chemicals and then stimulated with LPS 1 μg/mL for the indicated time in figure legends. Various concentrations of carnosol dissolved in ethanol were added together with LPS. The final concentration of ethanol used was less than 0.05%. Cells were treated with 0.05% ethanol as the vehicle control.

Lee D.Y., Hwang C.J., Choi J.Y., Park M.H., Song M.J., Oh K.W., Son D.J., Lee S.H., Han S.B, & Hong J.T. (2017). Inhibitory Effect of Carnosol on Phthalic Anhydride-Induced Atopic Dermatitis via Inhibition of STAT3. Biomolecules & Therapeutics, 25(5), 535-544.

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