Dee6500 2 cat

Arterial and femoral venous blood samples (1 ml each) were drawn into preheparinized syringes and analyzed immediately for blood gas variables, hemoglobin, electrolytes, lactate, and glucose (ABL 800 FLEX; Radiometer, Copenhagen, Denmark) with values corrected to blood temperatures measured simultaneously from the site of sampling in each vessel and the analyzer calibrated at regular intervals in accordance with manufacturer guidelines. Additional arterial and femoral venous blood samples from both legs were collected in 2-ml syringes and transferred to EDTA tubes, centrifuged, and separated. Plasma adrenaline and noradrenaline were subsequently determined using an enzyme-linked immunoassay kit (DEE6500 2-CAT; Demeditec Diagnostics GmbH, Kiel, Germany).

Arterial and jugular venous blood samples were drawn into pre-heparinised syringes and analysed immediately for blood gas variables (ABL 800 FLEX, Radiometer, Copenhagen, Denmark) corrected for blood temperature in the internal jugular vein. The analyser was calibrated at regular intervals in accordance with manufacturer guidelines. Additional arterial and jugular venous blood was collected in 2 ml syringes and transferred to EDTA tubes, centrifuged and separated. Plasma adrenaline and noradrenaline were subsequently determined using an enzyme immunoassay kit (DEE6500 2-CAT, Demeditec Diagnostics GmbH, Kiel, Germany). Blood samples were also collected directly in stop solution (Gorman et al. 2003 (link); Kalsi & González-Alonso, 2012 (link)). Plasma ATP was then determined using the luciferin–luciferase technique by a luminometer with three automatic injectors (Orion Microplate Luminometer, Bethold Detection System GmbH, Pforzheim, Germany).

Arterial and jugular venous blood samples were drawn into preheparinized syringes and analyzed immediately for blood gas variables (ABL 800 FLEX, Radiometer, Copenhagen, Denmark), corrected for blood temperature in the internal jugular vein. The analyzer was calibrated at regular intervals in accordance with manufacturer guidelines. Additional arterial and jugular venous blood were collected in 2-ml syringes, transferred to EDTA tubes, centrifuged, and separated. Plasma epinephrine and norepinephrine (NE) were subsequently determined using an enzyme-linked immunoassay kit (DEE6500 2-CAT, Demeditec Diagnostics).