Hyperswitch

Manufactured by IonOptix

Sourced in United States

The Hyperswitch is a high-speed electronic switch designed for the precise control of light sources in optical measurement systems. It is capable of switching light sources on and off with microsecond response times, enabling rapid and accurate control of light exposure.

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Lab products found in correlation

X71 inverted microscope, by Olympus (1 mentions) Calcium and contractility system, by IonOptix (1 mentions) Myocam s, by IonOptix (1 mentions) Ionwizard, by IonOptix (1 mentions)

Spelling variants of this product

HyperSwitch system (6 citations) Ionoptix HyperSwitch Myocyte Calcium and Contractility System (2 citations)

2 protocols using hyperswitch

Fura-2 Calcium Imaging Procedure

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Fura-2 loading was done by incubating the cells with 2.5 μM Fura-2/AM and 0.75 μM Pluronic-127 (in 20% DMSO) at room temperature for 30 minutes, followed by 45 minutes incubation. The dye loaded cells were perfused in Tyrode solution containing (in mM): 150 NaCl, 5.4 KCl, 1.2MgCl2, 1 CaCl2, 10 Glucose at pH 7.4 during the experiment. An IonOptix system with a Hyperswitch (IonOptix Inc., USA) and a Olympus X71 inverted microscope with a water immersion fluorescence objective UPlanSPao 40X, 1.15 NA (Olympus USA), were used for fluorescence excitation (340 nm and 380 nm) and emission acquisition (510/40nm filter) by photomultiplier tube (PMT).

Jian Z., Chen Y.J., Shimkunas R., Jian Y., Jaradeh M., Chavez K., Chiamvimonvat N., Tardiff J.C., Izu L.T., Ross R.S, & Chen-Izu Y. (2016). In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. PLoS ONE, 11(8), e0160605.

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Calcium Transients in Ventricular Myocytes

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Calcium tolerant right ventricular myocytes were loaded with fura2-AM
ratiometric dye (Sigma, St Louis, MO, USA) at room temperature. Calcium
transients were recorded at 34 ± 2°C using IonOptix Calcium and
Contractility System equipped with a Hyperswitch and MyoCam-S (IonOptix,
Westwood, MA, USA). Myocytes were paced 2–6 Hz with field pacing using
Myopacer (IonOptix, Westwood, MA, USA). Bath solution contained (mM): NaCl 148,
KCl 5.4, CaCl₂ 1.8, MgCl₂ 1, HEPES 15, NaHPO₄0.4, D-glucose 5.5 and pH adjusted to 7.4 (NaOH). Diastolic calcium ratio was
analyzed (IonWizard, IonOptix; Origin 6). Each myocyte was paced for 1 minute
before recording 10 consecutive calcium transients for analysis. Cells from WT
and R67Q^+/− mice were used and calcium transients measured at
baseline and following incubation with isoproterenol (10 nM). In a separate set
of experiments, calcium tolerant right ventricular myocytes were paced for 30 s
at 2 Hz then treated with caffeine (10 mM) and sarcoplasmic reticulum (SR)
calcium content and Na⁺/Ca²⁺-exchanger (NCX) activity were
analyzed.

Reilly L., Alvarado F.J., Lang D., Abozeid S., Van Ert H., Spellman C., Warden J., Makielski J.C., Glukhov A.V, & Eckhardt L.L. (2020). Genetic Loss of IK1 Causes Adrenergic-induced Phase 3 Early Afterdepolarizations and Polymorphic and Bi-directional Ventricular Tachycardia. Circulation. Arrhythmia and electrophysiology, 13(9), e008638.

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