Be2011

The Myc-IbEGF and IbCOP9-5α-GFP vectors were transiently expressed in N. benthamiana leaves. Total proteins were extracted from the leaves with extraction buffer, mixed with Myc magnetic beads (P2118, Beyotime) and then incubated at 4°C for 4 h (Zhang et al., 2020 (link)). The agarose was washed at least five times with extraction buffer and boiled in 5× SDS loading buffer for 15 min to separate the proteins from the agarose beads. The proteins were detected using polyclonal anti-Myc (1:10000, BE2011, EASYBIO) and anti-GFP antibodies (1:10000, BE2002, EASYBIO).

Mouse antibody to α‐Tubulin‐FITC (1:400, F2168) was purchased from Sigma (USA). Mouse antibodies to γ‐Tubulin (1:200, ab11316) and TPM3 (1:100, ab113692) were purchased from Abcam (USA). Mouse antibody to FLAG (1:2000, M20008L) was purchased from Abmart (Shanghai, China). Rabbit antibody to MYC (1:1000, BE2011) was purchased from EASYBIO (Beijing, China). Rabbit antibodies to β‐Actin (1:2000, AC026) and β‐Tubulin (1:1000, AC008) were purchased from ABclonal (Wuhan, China). Rabbit antibodies to RNF20 (1: 1000, 21625‐1‐AP) and TPM3 (1:1000, 10737‐1‐AP) were purchased from Proteintech Group (USA). Rabbit antibodies to H2Bub (1: 1000, 5546s), H2B (1: 1000, 12364S) and H3 (1: 1000, 4499S) were purchased from Cell Signaling Technology (USA). Mouse antibody to HEC1 (1: 100, sc‐515510) was purchased from Santa Cruz Biotechnology (USA). Rabbit antibodies to RBBP7 (1:100, bs8620) and UBASH3B (1:100, bs8741) were purchased from Bioworld (Beijing, China). Human antibody to ACA (1: 200, 15–234) was purchased from Antibodiesinc (USA). Goat anti‐rabbit FITC (1:200, ZF‐0311) and goat anti‐mouse TRITC (1:200, ZB‐2305) were purchased from ZSGB‐BIO (Beijing, China). Alexa Fluor 680‐conjugated goat anti‐mouse (1:10000, A21057) and Alexa Fluor 800‐conjugated goat anti‐rabbit (1:10000, A21109) were purchased from Invitrogen (USA).