Fitc affinipure donkey anti goat igg

Cryosections of the eyeballs were processed as previously reported [20 (link)]. Eyecups were immediately fixed in 4% paraformaldehyde (PFA) for about 30 min. After graded dehydration in 10% and 20% sucrose for 1 h, respectively, and then in 30% sucrose overnight at 4°C, the eyecups were embedded into the optimum cutting temperature medium (Sakura Finetek, CA, USA). The cryosections were cut vertically (12 μm) using Leika CM1950 crostat (Leika, Wetzlar, Germany). The sections were mounted on polylysine slides and immunostained with the Gal-1 antibody (5 μg/ml, R&D System, MN, USA) overnight at 4°C. After rinsing with PBS-T buffer (0.1% Triton-X 100), cryosections were then incubated with FITC-AffiniPure Donkey Anti-Goat IgG (1 : 200, Jackson ImmunoResearch Laboratories, PA, USA) for 1 h at room temperature. The images were taken by laser scanning microscopy (FV1200; Olympus, Tokyo, Japan).

PTPδ peptides (IQKLTQIETGENVTGMELEF) and PTPσ peptides (PIADMAEHTERLKANDSLK) were synthesized and conjugated to keyhole limpet hemocyanin through a cysteine added to the C-terminus of the PTPδ peptide or the N-terminus of the PTPσ peptide. After immunization of rabbits with the respective immunogen, specific PTPδ antibodies (JK123; 1 μg/ml; RRID: AB_2713995) and PTPσ antibodies (JK125; 1 μg/ml; AB_2713993) were affinity-purified using a Sulfolink column (Pierce). The following primary antibodies were obtained commercially, as indicated: Goat polyclonal anti-GFP antibody (1:1000 dilution; RRID: AB_218182; Cat# 600-101-215) was purchased from Rockland Immunocytochemicals. FITC-AffiniPure donkey anti-goat IgG (1:150 dilution; RRID: AB_230401; Cat# 705-095-147) was purchased from Jackson ImmunoResearch.