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The Sevag is a laboratory equipment used for the extraction and purification of nucleic acids, such as DNA and RNA, from various biological samples. It functions as a device to separate the organic and aqueous phases during the nucleic acid extraction process.

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Lab products found in correlation

Phenol, by Avantor (2 mentions) Phenol, by Thermo Fisher Scientific (2 mentions) Rnases, by Merck Group (2 mentions) Proteinase k, by Roche (2 mentions)

2 protocols using sevag

1

Solvent-Based DNA Extraction from Tissues

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DNA was isolated from the liver and lung tissues by a solvent extraction procedure (Gupta 1996 ) involving removal of RNA and proteins by digestion of isolated crude nuclei with RNases (Sigma Chemical Co., St. Louis, MO) and proteinase K (Roche Diagnostics, Indianapolis, IN,), respectively, followed by sequential extractions with phenol (Amresco, Solon, OH), phenol:Sevag (1:1) and Sevag (choloroform:isoamyl alcohol; 24:1) (Fisher Scientific, Pittsburgh, PA). DNA was recovered by precipitation with ethanol, washed, dissolved in water and its concentration and purity was estimated by spectrophotometry.
Aqil F., Shen H., Jeyabalan J., Xin X., Lehmler H.J., Ludewig G., Robertson L.W, & Gupta R.C. (2014). Sustained expression of CYPs and DNA adduct accumulation with continuous exposure to PCB126 and PCB153 through a new delivery method: Polymeric implants. Toxicology Reports, 1, 820-833.
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2

Solvent-Based DNA Extraction from Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was isolated from the liver and lung tissues by a solvent extraction procedure [16] involving removal of RNA and proteins by digestion of isolated crude nuclei with RNases (Sigma Chemical Co., St. Louis, MO) and proteinase K (Roche Diagnostics, Indianapolis, IN), respectively, followed by sequential extractions with phenol (Amresco, Solon, OH), phenol:Sevag (1:1) and Sevag (choloroform:isoamyl alcohol; 24:1) (Fisher Scientific, Pittsburgh, PA). DNA was recovered by precipitation with ethanol, washed, dissolved in water and its concentration and purity was estimated by spectrophotometry.
Aqil F., Shen H., Jeyabalan J., Xin X., Lehmler H.J., Ludewig G., Robertson L.W, & Gupta R.C. (2014). Sustained expression of CYPs and DNA adduct accumulation with continuous exposure to PCB126 and PCB153 through a new delivery method: Polymeric implants. Toxicology Reports, 1, 820-833.
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