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Eyelink toolbox

Manufactured by SR Research
Sourced in Canada

The Eyelink Toolbox is a software package designed to facilitate the integration of eye-tracking technology into research studies. It provides a set of tools and functions that allow researchers to interface with Eyelink eye-tracking systems and incorporate eye movement data into their experimental paradigms. The Toolbox is compatible with various programming environments, enabling researchers to collect and analyze eye-tracking data within their preferred research workflows.

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8 protocols using eyelink toolbox

1

Neural Correlates of Patch Leaving and Traveling Salesman Tasks

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Two monkeys were trained on both tasks, first the patch leaving task, followed by neural recordings, and then the traveling salesman task, followed by neural recordings. Neural recordings began once a stable pattern of behavior emerged, within two weeks of onset of training for both tasks. For the patch leaving task, we regarded behavior as stabilized when a significant influence of travel time on total time in patch emerged (cf. (Houston and McNamara 1999 ). For the traveling salesman task, we regarded behavior as stable when monkeys exhibited the same pattern of choices (a trapline) over the course of five behavioral sessions.
During training and recording, monkeys’ access to fluid was controlled outside of experimental sessions. Custom software written in MATLAB (Mathworks, Natick, MA, USA) using Psychtoolbox (Brainard 1997 (link)) controlled stimulus presentation, reward delivery, and recorded all task and behavioral events. Horizontal and vertical eye traces were sampled at 1000 Hz by an infrared eye-monitoring camera system (SR Research, Osgoode, ON) and recorded using the Eyelink toolbox (Cornelissen et al. 2002 ). Solenoid valves controlled juice delivery. All data were analyzed using custom software written in MATLAB.
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2

Visual Attention Study with Eyetracking

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The experiment was conducted using the Psychtoolbox (51 (link), 52 (link)) in MATLAB
(R2016a; The MathWorks, Natick, MA) and stimuli were presented on a
VIEWPixx monitor (VPIxx Technologies, Inc, Saint-Bruno, Quebec, Canada)
at a viewing distance of 60 cm. The monitor had a spatial resolution of
1920 x 1080 pixel and a size of 51.5 x 29 cm. Eye movements of the right
eye were recorded at 1000 Hz using a desktop mounted EyeLink 1000 (SR
Research Ltd., Ontario, Canada) and the EyeLink Toolbox (53 (link)). Due to
technical issues, the data of five participants were recorded at 2000 Hz
and downsampled to 1000 Hz before further analysis.
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3

Visual Stimulus Presentation and Eye Tracking

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The experiment was performed in a quiet and dark room. Subjects sat in front of a monitor screen (40 × 30 cm) at a distance of 57 cm, with their head stabilized by a chin rest. Stimuli were generated using the PsychoPhysics Toolbox routines (Brainard, 1997 (link); Pelli, 1997 (link)) for Matlab (MatLab r2010a, The Mathworks, inc.) and presented on a CRT monitor (Barco Calibrator Line) with a resolution of 800 × 600 pixel and a refresh rate of 120 Hz, driven by a Mac Pro 4.1.
Eye movements were monitored in-synch with visual presentations using the EyeLink 1000 system (SR Research, Canada) and the Eyelink toolbox for Matlab (Cornelissen et al., 2002 (link)). Eye position and pupil diameter of the left eye were measured with a frequency of 1000 Hz by means of an infrared sensor mounted below the screen, which allowed for unrestrained binocular viewing of the display. At the beginning of the experimental session, a standard 13-point calibration routine was performed.
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4

Visual Stimuli Presentation and Eye Tracking Protocol

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Stimuli were presented on an Eizo FlexScan 22-inch CRT monitor (Eizo, Hakusan, Japan) with a resolution of 1,152 × 870 pixels, a refresh rate of 75 Hz, and an effective display size of 40.7 × 30.5 cm. Participants viewed stimuli from a 67 cm distance. Head movements were restricted by means of a chin–forehead rest. Stimulus presentation was controlled via the Psychtoolbox (Brainard, 1997 (link); Kleiner et al., 2007 ) in MATLAB (The MathWorks, Natick, MA). Eye position of the right eye was recorded at 1000 Hz using the EyeLink 1000 (SR Research, Mississauga, ON, Canada) and the EyeLink Toolbox (Cornelissen et al., 2002 (link)). All stimuli were presented on a black background. The EyeLink was calibrated at the beginning of each block using a 9-point calibration protocol.
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5

Eye Tracking in Stereoscopic Experiments

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Experiments were conducted using the Psychtoolbox (Brainard, 1997 (link); Pelli, 1997 (link)) in MATLAB (The MathWorks, Natick, MA) and presented on a VIEWPixx monitor (VPixx Technologies Inc., Saint-Bruno, Quebec, Canada) at a viewing distance of 68.5 cm. The monitor had a spatial resolution of 1920 × 1080 pixels and a size of 51.5 × 29 cm. We recorded eye movements of both eyes using a desktop mounted EyeLink 1000 (SR Research Ltd., Ontario, Canada) with a sampling rate of 1000 Hz and the Eyelink Toolbox (Cornelissen, Peters, & Palmer, 2002 (link)). A mirror stereoscope (Wheatstone, 1838 (link)) consisting of four first surface mirrors with a diameter of 50.8 mm (Thorlabs Inc. Newton, NJ) was used to bring the views of the two eyes into alignment. The eye tracker was recording the eyes directly beneath the mirrors of the stereoscope.
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6

Monitoring Gaze Behavior on Linearized CRT

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Displays were presented on a linearized CRT monitor (Sony GDM-FW900) with resolution 1,024 × 640 pixels refreshing at 120 Hz. The monitor was viewed from 60 cm and the middle-gray background had a mean luminance of 56 cd/m2. Stimuli were created with MATLAB (MathWorks, Natick, MA, USA) and Psychophysics Toolbox (Brainard, 1997 (link)). Gaze position was monitored for all trials using an Eyelink 1000 (SR Research, Ottawa, Canada) and the Eyelink toolbox (Cornelissen et al., 2002 (link)). Trials without good fixation (e.g., broken fixations or blinks) were excluded from analysis. Such excluded trials were infrequent; across participants, they occurred on 1.9 ± 0.4% of trials for all conditions.
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7

Eye Tracking Stimulus Presentation

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Stimuli were presented on an Eizo FlexScan 22-inch CRT monitor (Eizo, Hakusan, Japan) with a resolution of 1152 × 870 pixels, a refresh rate of 75 Hz, and an effective display size of 40.7 × 30.5 cm. Participants viewed the monitor from a 67 cm distance. We controlled stimulus presentation via the Psychtoolbox (Brainard, 1997 (link); Kleiner et al., 2007 ) in MATLAB (The MathWorks, Natick, MA) and recorded eye position of the right eye at 1000 Hz using the EyeLink 1000 (SR Research, Mississauga, ON) and the EyeLink Toolbox (Cornelissen et al., 2002 (link)). All stimuli were presented on a uniform gray background.
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8

Eye Tracking for Probabilistic Perception

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We recorded eye movements as subjects watched sequential visual displays designed to elicit probabilistic expectations, following methods employed in preverbal infants [16 (link)] and gaze-based experimental protocols for studying animal visual perception and cognition [27 (link)]. Eye positions were measured with the Eyelink Toolbox and were sampled at 1000 Hz by an infrared eye-monitoring camera system (SR Research, Osgoode, ON, Canada) [28 (link)]. A solenoid valve delivered a 53 µl water reward when each object was at its peak (every 1.5 s), regardless of where or whether the subject was looking. The intermittent and fully predictable reward is a standard procedure in primate behaviour studies designed to increase general task participation without making any particular task events reward associated [26 (link)]. Regardless of subjects' gaze behaviour, each sequence was displayed in full. The rate of presentation was between 0 and 2 trials, interspersed within unrelated trials for other studies [26 (link),29 (link),30 (link)].
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