Hsc70 1b5 antibody

Manufactured by Enzo Life Sciences

The Hsc70 (1B5) antibody is a monoclonal antibody that specifically recognizes the heat shock cognate 70 kDa protein (Hsc70), a member of the heat shock protein 70 (Hsp70) family. Hsc70 is a constitutively expressed chaperone protein involved in the folding and trafficking of cellular proteins.

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Lab products found in correlation

Lamp 1 antibody, by Abcam (1 mentions) Transferrin, by Thermo Fisher Scientific (1 mentions) Tgn46 antibody, by Bio-Rad (1 mentions) Flag m2 antibody, by Merck Group (1 mentions) Erlotinib, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

HSC70 (16 citations)

2 protocols using hsc70 1b5 antibody

Immunofluorescence Antibody Panel Protocol

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Mouse monoclonal Flag (M2) antibody was obtained from Sigma-Aldrich. Rabbit polyclonal GFP (A6455), AF647-conjugated Phalloidin, transferrin, and anti–sheep, and Alexa Fluor 488–conjugated anti–mouse and anti–rabbit were purchased from Invitrogen. Chicken polyclonal Rab13 and rabbit monoclonal LAMP-1 antibodies were obtained from Abcam. Rat monoclonal Hsc70 (1B5) antibody was obtained from Enzo Life Sciences. Rabbit polyclonal TIP47 antibody was purchased from Novus Biologicals. Sheep polyclonal TGN46 antibody was obtained from AbD Serotec. HRP-conjugated anti–mouse and anti–rabbit were obtained from Jackson ImmunoResearch Laboratories, Inc.

Ioannou M.S., Bell E.S., Girard M., Chaineau M., Hamlin J.N., Daubaras M., Monast A., Park M., Hodgson L, & McPherson P.S. (2015). DENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior. The Journal of Cell Biology, 208(5), 629-648.

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Antibody Immunoblotting Protocols

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Rabbit polyclonal GFP (A6455) antibody was purchased from Invitrogen. Mouse monoclonal Arf5 (M01) antibody was obtained from Abnova. Rat monoclonal Hsc70 (1B5) antibody was purchased from Enzo LifeSciences. Mouse monoclonal pan Arf (1D9) antibody was purchased from Abcam. Rabbit polyclonal Rab35 antibody was raised against GST-tagged full-length human Rab35 as previously described (Allaire et al., 2010; (link)Allaire et al., 2013) (link). Rabbit polyclonal antibody recognizing GST was previously described (McPherson et al., 1994) . Rabbit monoclonal EGFR (D38B1) and phospho EGFR Y1068 (D7A5) and rabbit polyclonal phospho EGFR Y1148 antibodies were from Cell Signaling Technology. Mouse monoclonal actin (C4) was purchased from Millipore Sigma.
Cyclohexamide was obtained from Millipore Sigma and Erlotinib was from Selleckchem.

Kulasekaran G., Chaineau M., Piscopo V.E.C., Verginelli F., Laflamme C., Girard M., Tang Y., Dali R., Lo R., Stifani S., & McPherson P.S. (2020). An Arf/Rab cascade controls the growth and invasiveness of glioblastoma.

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