Anti ha tag antibody ab9110

The following human protein-coding plasmids were used for the transient expression in HEK293 cells: PAR1was purchased from cDNA Resource Center (Bloomsberg, PA, USA), PAR1-Rluc was used as previously described (Ayoub et al., 2010 (link)), HA-AT1R was kindly provided by Reiter E. (INRA, Nouzilly, France), AT1R-Rluc was a gift from Laporte, S. (McGill University, Montréal, Canada), yPET-β-arrestin 2 was kindly provided by Scott, M. (Cochin Institute, Paris, France), Grb2-Rluc and Grb2-Venus were kindly given by Prof. Pfleger KD. (Harry Perkins Institute of Medical Research and UWA, Perth, Australia), and Venus-Gαq, Venus-Kras, and Venus-Rabs that were generously shared by Lambert, N. (Augusta University, GA, USA). AngII, thrombin, and irbesartan were from Sigma (St Louis, MO, USA). SCH79797 was from Tocris (Tocris, Ellisville, MO). The IP1 kit was purchased from Cisbio Bioassays (PerkinElmer, Codelet, France) and the anti-HA tag antibody (ab9110) was purchased from abcam (abcam, MA, USA).

The peptide ligands parathyroid hormone PTH (1 (link)–34 (link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, no link found, no link found, link, link, link, link, link)) (human, H-4835-GMP, 4033364) and parathyroid hormone-related peptide (PTHrP) (1 (link)–34 (link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, no link found, no link found, link, link, link, link, link)) (human, mouse, rat; 4017147) were from Bachem. SNAP-Cell 647-SiR (S9102S) was from New England Biosciences. Anti–HA-tag antibody (ab9110) was from Abcam and anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (7074P2) was from Cell Signaling. The 3,3′,5,5′-tetramethylbenzidine (TMB, T8665) was from Sigma-Aldrich. NanoBRET Nano-Glo Substrate–furimazine (N1663) and HaloTag NanoBRET 618 Ligand (G9801) were from Promega. Dimethyl sulfoxide (A994.2) for cell culture was from Carl Roth GmbH & Co. KG. Bovine serum albumin (BSA, SAFSA7030) was from VWR International.

Cells were lysed in lysis buffer containing 50 mM HEPES, 150 mN NaCl (4.38 g), 1 mM EDTA, 1% (w/v) CHAPS and Sigma protease inhibitor cocktail. Subsequently, the cell lysates were boiled in 2 × SDS–PAGE loading buffer for 10 min, and then resolved by 10% SDS–PAGE and transferred to the nitrocellulose membrane. All primary antibodies were diluted 1,000 times for western blotting if not specified. The following antibodies were used in this study: TEAD4 (#ab58310) antibody, anti-Myc tag antibody (#ab9106) and anti-HA tag antibody (#ab9110) were purchased from Abcam; N-cadherin (#610921) antibody was purchase from BD; Vimentin (#5741) and YAP (#12395) antibodies were purchased from Cell Signaling Technology; and alpha-tubulin (#T5168, 1:5000 dilution) and FLAG M2 (#F1804) were purchased from Sigma-Aldrich. RBM4 antibody (#11614-1-AP) was purchased from Proteintech. Bound antibodies were visualized using the ECL kit (GE Healthcare).