Mouse anti α tubulin ab

Cells were washed with cold DPBS for two times and then lysed in 2× Laemmli buffer (2% SDS, 20% glycerol, and 125 mM Tris-HCl, pH 6.8) supplemented with 1× protease inhibitor cocktail (Sigma, P8340). The cell lysate was scraped and sonicated, and the concentration of protein was determined by BCA assay. The protein was separated by SDS-PAGE and transferred onto nitrocellulose membrane. The membrane was then blocked with 5% non-fat milk for 1 h at room temperature, and incubated with the indicated primary antibody overnight at 4 °C with shaking. The membrane was washed for 3 times and incubated with secondary antibodies for 2 h. The signal was then detected with ECL substrates (Millipore, WBKLS0500). Dilutions of antibodies were: rabbit anti-ARID1A Ab (1:1000, Abcam, ab182560), rabbit anti-BRD4 Ab (1:1000, Active Motif, 39909), mouse anti-α-Tubulin Ab (1:3000, Cell Signaling, 3873 s). Goat anti-Rabbit IgG (H + L) Secondary Antibody (1:5000, Thermo Fisher Scientific, 31460), Goat anti-Mouse IgG (H + L) Secondary Antibody (1:5000, Thermo Fisher Scientific, 31430). The full scan blots were included in the Source data file.