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Iron 2 sulfate

Manufactured by Merck Group
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Iron (II) sulfate is a chemical compound with the formula FeSO4. It is a crystalline solid that is soluble in water, and is commonly used as a source of iron in various applications. The core function of iron (II) sulfate is to provide a source of iron, which is an essential mineral for various biological processes.

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Lab products found in correlation

Ascorbic acid, by Merck Group (7 mentions) Sulfuric acid, by Merck Group (7 mentions) Gallic acid, by Merck Group (6 mentions) Iron 3 chloride, by Merck Group (6 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (6 mentions) Sodium hydroxide (naoh), by Merck Group (6 mentions) Hydrogen peroxide, by Merck Group (6 mentions) Acetonitrile, by Merck Group (6 mentions) Folin ciocalteu reagent, by Merck Group (5 mentions) Ammonium molybdate, by Merck Group (5 mentions) Methionine, by Merck Group (5 mentions) Hydrochloric acid (hcl), by Merck Group (4 mentions) Ethanol, by Merck Group (4 mentions) Sodium acetate, by Merck Group (4 mentions) Quercetin, by Merck Group (3 mentions) Catechin, by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Nitroblue tetrazolium, by Merck Group (3 mentions) Methanol, by Merck Group (3 mentions) Riboflavin, by Merck Group (3 mentions)

Close spelling variants of this product

Iron(II) sulfate heptahydrate (FeSO4·7H2O) Ammonium iron (II) sulfate Iron(II) sulfate (FeSO4), Iron (II) sulfate tetrahydrate Iron (II) sulfate 7-hydrate Ammonium iron(II) sulfate hexahydrate Iron(II) sulfate heptahydrate Iron sulfate Iron (II)

27 protocols using iron 2 sulfate

1

Oxidative Stress Biomarker Assay

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Dimethyl sulfoxide (DMSO), hydrogen peroxide (H2O2), 5,5′-dithio-bis(2-nitro-benzoic acid) (DTNB), 2-4-dinitrophenolhydrazine (DNPH), thiobarbituric acid (TBA), versenic acid (EDTA) and Iron (II) sulfate were purchased from Sigma-Aldrich (St. Louis, MO., USA). Trichloroacetic acid (TCA), hydrochloric acid (HCl), sodium chloride (NaCl), ethanol 96%, ethyl acetate, guanidine hydrochloride were acquired from POCh (Gliwice, Poland) and Alfachem (Poznań, Poland). Thrombin was purchased from Biomed (Lublin, Poland). Reagents for PT and APTT were acquired from Kselmed (Grudziąc, Poland).
Żuchowski J., Skalski B., Juszczak M., Woźniak K., Stochmal A, & Olas B. (2020). LC/MS Analysis of Saponin Fraction from the Leaves of Elaeagnus rhamnoides (L.) A. Nelson and Its Biological Properties in Different In Vitro Models. Molecules, 25(13), 3004.
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2

Comprehensive Reagent Inventory for Assays

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The following reagents were used in this study: Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, gallic acid, catechin, trolox, iron(II)sulfate, tryptic soy broth, ketoconazole, streptomycin, phosphate-buffed saline (PBS), and iron(III)chloride were purchased from Sigma Aldrich (St. Louis, MO, USA); sodium-carbonate from FHI Zdravlje AD Leskovac (Serbia); sodium nitrite from Alkaloid Skopje (Skopje, Macedonia); aluminum chloride, and tri-chloroacetic acid from Kemika (Zagreb, Croatia); sodium hydroxide from NRK Inzenjering (Belgrade, Serbia); 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid)-ABTS from Roche Diagnostics GmbH (Penzberg, Germany); neocuproin from Acros Organics (Geel, Belgium); monosodium phosphate and disodium phosphate from Merck (Boston, MA, USA); cuprum chloride from Fluka (Buchs, Switzerland); ammonium acetate and ethanol from Zorka Pharma-Hemija (Šabac, Serbia); gentamicin from Panfarma (Belgrade, Serbia); crystal violet from Bio-Merieux (France); methanol and ferrous sulfate from Zorka Šabac, Serbia; high-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS); 2 mM L-glutamine from Thermo Fisher Scientific (Altrincham, UK); and penicillin as bought from Panfarma, Šabac, Serbia.
Marković T., Čutović N., Carević T., Gašić U., Stojković D., Xue J, & Jovanović A. (2023). Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. International Journal of Molecular Sciences, 24(14), 11764.
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3

Glycation Inhibition and Antioxidant Assay

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Bovine serum albumin (BSA), D-fructose, D-ribose, methylglyoxal (MGO), L-alanine, aminoguanidine hydrochloride (AG), 1,2-phenylenediamine (PD), 2,3-dimethylquinoxaline (DQ), α-cyano-4-hydroxy-cinnamic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), trifluoroacetic acid (TFA), ascorbic acid, 2,2′-bipyridyl, sodium dodecyl sulfate, and iron (II) sulfate were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). G.K. peptide was purchased from Bachem Americas, Inc. (Torrance, CA, USA) and all HPLC solvents were purchased from Thermo Fisher Scientific (Rockford, IL, USA).
Ma H., Liu W., Frost L., Kirschenbaum L.J., Dain J.A, & Seeram N.P. (2016). Glucitol-core containing gallotannins inhibit the formation of advanced glycation end-products mediated by their antioxidant potential. Food & function, 7(5), 2213-2222.
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4

Antioxidant and Enzymatic Assays

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Bovine serum albumin (BSA), aminoguanidine (AG), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (Trolox), 2,4,6- tripyridyl-S-triazine (TPTZ), iron (II) sulfate (FeSO4), xanthine, xanthine oxidase, 5,5′-dithiobisnitro benzoic acid (DTNB), nitroblue tetrazolium (NBT), 1-deoxy-1-morpholinofructose (DMF), 2,4-dinitrophenylhydrazine (DNPH), thioflavin T reagent (4-(3,6-dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylaniline chloride), and L-cysteine were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Fructose, Folin-Ciocalteu’s phenol reagent, and gallic acid were purchased from Fluka (St. Louis, MO, USA). All other chemical reagents used in this study were of analytical grade.
Chayaratanasin P., Barbieri M.A., Suanpairintr N, & Adisakwattana S. (2015). Inhibitory effect of Clitoria ternatea flower petal extract on fructose-induced protein glycation and oxidation-dependent damages to albumin in vitro. BMC Complementary and Alternative Medicine, 15, 27.
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5

Phytochemical Analysis and Antioxidant Evaluation

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The following chemical reagents were used in the present experiments: dimethyl sulfoxide (DMSO, ≥99%, Stanlab, Lublin, Poland), methanol (≥99%, Stanlab, Lublin, Poland), Folin-Ciocalteu reagent (AKTYN, Suchy Las, Poland), sodium carbonate (≥99%, Stanlab, Lublin, Poland), gallic acid (≥98%, Sigma Aldrich, Poznań, Poland), 2,2-diphenyl-1-picrylhydrazyl, (DPPH, Sigma Aldrich, Poznań, Poland), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, ≥98%, Sigma Aldrich, Poznań, Poland), iron(II)sulfate (≥99%, Sigma Aldrich, Poznań, Poland), ferrosine (97%, Sigma Aldrich, Poznań, Poland), ethylenediaminetetraacetic acid (EDTA, ≥ 99%, Sigma Aldrich, Poznań, Poland), diethyl ether (≥98%, Sigma Aldrich, Poznań, Poland), aluminium chloride hexahydrate (≥99%, POCH), quercetin (≥95%, Sigma Aldrich), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma Aldrich, Poznań, Poland), potassium persulfate (≥99%, Sigma Aldrich, Poznań, Poland), acetonitrile (≥99.9%, Sigma Aldrich, Poznań, Poland), formic acid (≥98%, Sigma Aldrich, Poznań, Poland), hydrochloric acid (Fluka, Poznań, Poland) and sodium hydroxide (Sigma Aldrich, Poznań, Poland).
Ostolski M., Adamczak M., Brzozowski B, & Wiczkowski W. (2021). Antioxidant Activity and Chemical Characteristics of Supercritical CO2 and Water Extracts from Willow and Poplar. Molecules, 26(3), 545.
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6

Phantom Validation for Quantitative Susceptibility Mapping

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Five phantoms with various iron compounds and iron containing molecules were constructed for validating QSM acquisition and reconstruction. Each phantom contained seven vials with linearly increasing concentrations of the iron-containing material. Phantom #1 contained Ferumoxytol (carboxymethyl-dextran coated ultra small super paramagnetic iron oxide, Advanced Magnetics, Cambridge, MA) with concentrations of (0, 10, 20, 30, 40, 50, 60) μg Fe/mL. Phantom #2 contained ferric iron (Iron(III) chloride, Sigma-Aldrich) with concentrations of (0, 100, 300, 500, 700, 900, 1200) μg Fe/mL. Phantom #3 contained ferrous iron (Iron(II) sulfate, Sigma-Aldrich) with concentrations of (0, 100, 300, 500, 700, 900, 1200) μg Fe/mL. Phantom #4 contained extracted human hemoglobin (Sigma-Aldrich) with concentrations of (0, 10, 20, 30, 40, 50, 60) μg Fe/mL. Phantom #5 contained ferritin from equine spleen (Sigma-Aldrich) with approximate concentrations of (0, 29, 145, 290, 435, 580, 725) μg Fe/mL, assuming 29% w/w (iron/ferritin). Vials in each phantom were all immersed in a water container.
Tan H., Liu T., Wu Y., Thacker J., Shenkar R., Mikati A.G., Shi C., Dykstra C., Wang Y., Prasad P.V., Edelman R.R, & Awad I.A. (2014). Evaluation of Iron Content in Human Cerebral Cavernous Malformation using Quantitative Susceptibility Mapping. Investigative radiology, 49(7), 498-504.
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7

Antioxidant Activity of Tannin Extracts

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Chestnut tannins (CT) and quebracho tannins (QT) were provided by Silvateam (S. Michele Mondovì, Cuneo, Italy).
Whatman® Nylon membrane filters (0.45 μm pore size, 47 mm diameter) were from Sigma-Aldrich (Milan, Italy).
2,2-Diphenyl-1-picrylhydrazyl (DPPH), iron (III) chloride hexahydrate (97%), iron (II) chloride tetrahydrate, iron (II) sulfate, 2,4,6-tris(2-pirydyl)-s-triazine (TPTZ) (≥98%), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) (97%), sodium borohydride, and ethylenediaminetetraacetic acid (EDTA) sodium salt were obtained from Sigma-Aldrich (Milan, Italy) and used as obtained.
The laccase used in this study was the recombinant POXA1b from the fungus Pleurotus ostreatus heterologously produced in the yeast Pichia pastoris [28 (link)].
UV-Vis spectra were recorded using a HewlettPackard 8453 Agilent spectrophotometer.
Chemical structures were drawn with the software ChemDraw Ultra 12.0.
Moccia F., Piscitelli A., Giovando S., Giardina P., Panzella L., d’Ischia M, & Napolitano A. (2020). Hydrolyzable vs. Condensed Wood Tannins for Bio-based Antioxidant Coatings: Superior Properties of Quebracho Tannins. Antioxidants, 9(9), 804.
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8

Copper(II) Complex Characterization

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Copper(II) chloride dihydrate; 3,4-difluorophenylacetic acid; 2-methylpyridine; 3-methylpyridine; 2,2-diphenyl-1-picrylhydrazyl radical (DPPH); ascorbic acid; iron(II) sulfate; hydrogen peroxide; nitric acid; sodium salt of the salmon sperm DNA (SS-DNA); methanol; and DMSO were purchased from Sigma-Aldrich, St. Louis, MO, USA. These chemicals were used without any further treatment. Distilled water was used for the experimental work. The melting points of the synthesized complexes were recorded in a capillary tube using a digital electro-thermal melting point apparatus. Elemental analysis was performed on a Leco CHNS 932. A Perkin Elmer atomic absorption spectrometer A analyst 700 was used to determine the percentage of copper. The electronic absorption spectra (200–800 nm) of the complexes were recorded using a Perkin Elmer UV/Vis spectrometer Lambda 25 in DMSO solvent. A nicolet-6700 FT-IR spectrophotometer (Thermo Scientific, Waltham, MA, USA) was used to record FT-IR spectra in the range of 4000–400 cm−1, adopting the attenuated total reflectance (ATR) technique.
V.i.o.l.a., Muhammad N., Noor A., Sirajuddin M., Kubicki M., Rahim S., Samad A., Shujah S., Wadood A, & Ali S. (2023). Designing and Exploration of the Biological Potentials of Novel Centrosymmetric Heteroleptic Copper(II) Carboxylates. Pharmaceuticals, 16(10), 1462.
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9

Comprehensive Antioxidant Activity Assays

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The following reagents were used: Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, gallic acid, catechin, Trolox, iron(II)sulfate, and iron(III)chloride were bought from Sigma Aldrich (USA), sodium-carbonate from Zdravlje (Serbia), sodium nitrite from Alkaloid Skopje (Macedonia), aluminum chloride and trichloroacetic acid were from Kemika (Croatia), sodium hydroxide from NRK Inzenjering (Serbia), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS was from Roche Diagnostics GmbH (Germany), neocuproin was from Acros Organics (Belgium), monosodium phosphate and disodium phosphate were from Merck (USA), cuprum chloride was from Fluka (Germany), and ammonium acetate and ethanol were from Zorka Pharma (Serbia).
Čutović N., Marković T., Kostić M., Gašić U., Prijić Ž., Ren X., Lukić M, & Bugarski B. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. Pharmaceuticals, 15(12), 1537.
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10

Hypoxia Induction and Compound Preparation

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Cycloheximide (CHX; Sigma-Aldrich) was dissolved in ethanol; desferrioxamine (DFX; Sigma-Aldrich), (+)-sodium l-ascorbate (Sigma-Aldrich), 2-oxoglutarate (Sigma-Aldrich), diethyl 2-oxoglutarate (DE-2OG; Sigma-Aldrich) and iron (II) sulfate (Sigma-Aldrich) in H2O; and dimethyloxalylglycine (DMOG; Frontier Scientific, Logan, UT, USA) and FG-4592 (Roxadustat; Selleckchem, Houston, TX, USA) in dimethylsulfoxide (DMSO, Sigma-Aldrich). Hypoxic incubations were performed using the InvivO2 400 humidified cell culture workstation (Baker Ruskinn, Bridgend, South Wales, UK) operated at 0.2% O2 and 5% CO2 as described previously [29 (link)] or in humidified oxygen-regulated cell culture incubators (Binder GmbH, Tuttlingen, Germany) operated at 1%–8% O2 and 5% CO2. If not otherwise indicated, “normoxia” refers to the standard oxygen concentration in the gas phase within a cell culture incubator at 500 m altitude (18.5% O2) and “hypoxia” to 0.2% O2 [30 (link)].
Pickel C., Günter J., Ruiz-Serrano A., Spielmann P., Fabrizio J.A., Wolski W., Peet D.J., Wenger R.H, & Scholz C.C. (2019). Oxygen-dependent bond formation with FIH regulates the activity of the client protein OTUB1. Redox Biology, 26, 101265.
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