A2228

Manufactured by Abcam

Sourced in United States

A2228 is a lab equipment product. It is a device used for the measurement and analysis of various samples in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Ab90532, by Abcam (1 mentions) Hrp conjugated goat anti rabbit igg, by Merck Group (1 mentions) Mouse anti gapdh, by Merck Group (1 mentions) Ab2074, by Abcam (1 mentions) Ab97672, by Abcam (1 mentions) Ab15087, by Abcam (1 mentions) Ab15093, by Merck Group (1 mentions) Ab5535, by Abcam (1 mentions) Hrp goat anti rabbit igg, by Proteintech (1 mentions) Dylight 550, by Thermo Fisher Scientific (1 mentions) Hrp goat anti mouse igg, by ABclonal (1 mentions) Dylight 488, by Thermo Fisher Scientific (1 mentions) Sa00001, by Proteintech (1 mentions) Sc 6248, by Santa Cruz Biotechnology (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions) F3165, by Merck Group (1 mentions) Ab20737, by Abcam (1 mentions) Nupage, by Thermo Fisher Scientific (1 mentions) Immobilon fl pvdf membrane, by Merck Group (1 mentions) Odyssey ir imaging system, by LI COR (1 mentions)

4 protocols using a2228

Immunoblotting of Phosphorylated NF-κB

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Immunoblotting of lysate cells was carried out accordingly^{38 (link)}. Very briefly, PVDF membranes (PVDF; Biorad, Hercules, CA, USA) were incubated with primary antibodies (1:5000 dilution of mouse anti-GAPDH (Sigma-Aldrich, A2228)) and rabbit anti-NF-κB/P65 antibody (Abcam, ab90532, dilution: 1/500) that specifically recognizes phosphorylated NF-κB for 90 min. Subsequent to washing, membranes were incubated again with horseradish peroxidase (HRP)-conjugated goat antibody against mouse IgG (BBI, Boston Biomedical Inc., Cambridge, MA, USA) at a dilution of 1:16,000, or HRP-conjugated goat anti-rabbit IgG (Sigma-Aldrich) at a dilution of 1:5000 for 45 min.

Pakravan G., Foroughmand A.M., Peymani M., Ghaedi K., Hashemi M.S., Hajjari M, & Nasr-Esfahani M.H. (2018). Downregulation of miR-130a, antagonized doxorubicin-induced cardiotoxicity via increasing the PPARγ expression in mESCs-derived cardiac cells. Cell Death & Disease, 9(7), 758.

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Western Blot Analysis of CXCR4 in BMSCs

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BMSCs cell lysates were prepared in Complete Lysis-M EDTA-free buffer containing protease inhibitors (Roche Diagnostics, Indianapolis, IN, USA) and western blots performed as described previously (Periyasamy-Thandavan et al. 2008; Periyasamy-Thandavan et al. 2012). The same amounts of protein (25μg) were loaded for each lane for reducing electrophoresis. The resolved proteins were then electroblotted onto polyvinylidene difluoride membranes. The blots were incubated in a blocking buffer with 5% fat-free milk, and exposed to the primary antibodies overnight at 4 °C, and finally incubated with the horseradish peroxidase-conjugated secondary antibody to reveal antigens using an enhanced chemiluminescence kit from Pierce. Primary antibodies were used for actin (Sigma; A2228) diluted 1:50000 and CXCR4 (Abcam; ab2074) diluted 1:250.

Periyasamy-Thandavan S., Herberg S., Arounleut P., Upadhyay S., Dukes A., Davis C., Johnson M., McGee-Lawrence M., Hamrick M.W., Isales C.M, & Hill W.D. (2015). Caloric Restriction and the Adipokine Leptin alter the SDF-1 signaling axis in Bone Marrow and in Bone Marrow Derived Mesenchymal Stem Cells. Molecular and cellular endocrinology, 410, 64-72.

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Antibody Verification for Meiotic Protein Analysis

Cited 1 time

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The following antibodies were used: rabbit polyclonal antibody against TERB1 (1:1000)^{21 (link)}, TERB2 (this study, 1:2000), MAJIN (this study, 1:2000), SUN1 (this study, 1:1000), Speedy A (this study, 1:3000), phos-HistoneH2A.X (Ser139) (Millipore, 05-636-25UG, 1:2000), SYCP1 (Abcam, ab15087, 1:1000), SYCP3 (Abcam, ab15093, 1:2000), SOX9 (Millipore, ab5535, 1:200), MSH4 (Abcam, ab58666, 1:500), c-Myc (Santa Cruz, sc-789, 1:1000), TRF1 (1:1000)^{40 (link)}, mouse monoclonal antibodies against MLH1 (BD, 550838, 1:200), SYCP3 (Abcam, ab97672, 1:2000), TRF1 (Abcam, ab66223, 1:1000), c-Myc (Santa Cruz, sc-40, 1:1000), FLAG (Sigma, F3165, 1:200), actin (Sigma, A2228, 1:2000), DMC1 (Abcam, ab11054, 1:100), Lamin B1 (Proteintech, 66095-1-Ig, 1:500), CDK2 (Santa Cruz, sc-6248, 1:100). Secondary antibody for western blot: Goat anti-Mouse IgG/HRP (Abclonal, AS003, 1:2000), Goat anti-Rabbit IgG/HRP (Proteintech, SA00001, 1:2000). Goat anti-mouse/rabbit secondary antibody for IF: DyLight 488 (Thermo, 35502, 35553, 1:500), DyLight 550 (Thermo, 84540, 84541, 1:500), DyLight 633 (thermos, 35512, 35562, 1:500).

Wang Y., Chen Y., Chen J., Wang L., Nie L., Long J., Chang H., Wu J., Huang C, & Lei M. (2019). The meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelope. Nature Communications, 10, 564.

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Proteomic Analysis of Metabolic Tissues

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Liver (25 μg), brown adipose tissue (BAT) (20 μg), and gonadal WAT (gWAT) (20 μg) homogenized in PBS, 2 mg/ml BSA, and 5 U/ml heparin and isolated TRLs (5 μg) were analyzed by SDS-PAGE on 4–12% Bis-Tris gradient gels (NuPage; Invitrogen) with an equal amount of protein loading. Proteins were visualized by silver staining (Pierce) or after transfer to Immobilon-FL PVDF membrane (Millipore). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences) for 30 min and incubated overnight at 4°C with respective antibodies. Goat, mouse, and rabbit antibodies were incubated with secondary Odyssey IR dye antibodies (1:14,000) and visualized with an Odyssey IR imaging system (LI-COR Biosciences). Western blot primary antibodies included: mouse anti-mouse β-actin (Sigma, A2228; 1:5,000), rabbit anti-mouse apoB (Abcam, ab20737; 1:1,000), rabbit anti-mouse apoC-III (IONIS Pharmaceuticals; 1:2,000) (29 (link)), rabbit anti-mouse apoE (Meridian Life Sciences, K23100R; 1:1,000), and goat anti-mouse LPL (provided by S. Young, University of California, Los Angeles; 10 μg/ml).

Ramms B., Patel S., Nora C., Pessentheiner A.R., Chang M.W., Green C.R., Golden G.J., Secrest P., Krauss R.M., Metallo C.M., Benner C., Alexander V.J., Witztum J.L., Tsimikas S., Esko J.D, & Gordts P.L. (2019). ApoC-III ASO promotes tissue LPL activity in the absence of apoE-mediated TRL clearance. Journal of Lipid Research, 60(8), 1379-1395.

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