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Anti phospho stat1

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Anti-phospho-STAT1 is a laboratory reagent used to detect the phosphorylated form of the STAT1 protein. STAT1 is a transcription factor that plays a crucial role in cellular signaling pathways. The anti-phospho-STAT1 antibody specifically recognizes the phosphorylated version of STAT1, enabling researchers to study the activation and regulation of this important protein.

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Lab products found in correlation

Anti phospho akt ser473, by Cell Signaling Technology (2 mentions) Anti phospho axl, by Cell Signaling Technology (2 mentions) Anti phospho akt thr308, by Cell Signaling Technology (1 mentions) Human egf, by Thermo Fisher Scientific (1 mentions) Anti hgas6, by R&D Systems (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti axl, by Cell Signaling Technology (1 mentions) Anti zo 1, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti zeb1, by Cell Signaling Technology (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

STAT1 (19 citations) Anti-STAT1 (15 citations) Phospho-STAT1 (6 citations) Anti-phospho STAT1 (pY701) (2 citations)

2 protocols using anti phospho stat1

1

Receptor Tyrosine Kinase Signaling Assay

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Antibodies used were as follows: anti-hEGFR (Santa Cruz, Sc-120), PE-Mouse anti- hEGFR (BD Biosciences, 555997), anti-hTyro3 (Cell Signaling, D38C6), anti- phosphor-hTyro3 (Aviva Systems Biology, OAAF00456), anti-hAxl (Cell Signaling, C89E7), anti-phospho-Axl (Cell Signaling, D12B2), anti-hMer (Cell Signaling, D21F11), anti-phospho-Mer (FabGennix, PMKT-140AP), anti-phospho-STAT1 (BD Bioscience, 612233), anti-phospho-Akt (Ser473) (Cell Signaling, 193H12), anti-phospho-Akt (Thr308) (Cell Signaling, D25E6), anti-phospho-Erk1/2 (Cell Signaling, 20G11), anti-β-Actinin (Cell Signaling, MAB374), and anti-phosphotyrosine pY99 (Millipore, 05–321), anti-hGas6 (R&D Systems, AF986), Human EGF (Invitrogen, PHG0311). The secondary antibodies used for immunoblot analysis were horseradish peroxidase–conjugated Affinipure Goat anti-mouse (Jackson ImmunoResearch, 115-035-166) and anti-rabbit (Jackson ImmunoResearch, 111-035-144).
Kimani S.G., Kumar S., Davra V., Chang Y.J., Kasikara C., Geng K., Tsou W.I., Wang S., Hoque M., Boháč A., Lewis-Antes A., De Lorenzo M.S., Kotenko S.V, & Birge R.B. (2016). Normalization of TAM post-receptor signaling reveals a cell invasive signature for Axl tyrosine kinase. Cell Communication and Signaling : CCS, 14(1), 19.
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2

Comprehensive Protein Analysis in Cell Lysates

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Whole cell lysates of cells were prepared in HNTG buffer (20mM HEPES, pH 7.5, 150mM NaCl, 10% glycerol) supplemented with 1% Triton X-100, 1mM PMSF, 1mM sodium orthovanadate, 10mM sodium molybdate, 1mM EDTA, 10mM sodium fluoride and 1% protease inhibitor cocktail. Western blot analyses were performed using antibodies as follows: anti-Axl (SCBT), anti-phospho-Axl (Sigma), anti-phospho-Akt (Ser473) (Cell Signaling, 193H12), anti-Vimentin (Cell Signaling, D21H3), anti-E-Cadherin (Cell Signaling, 24E10), anti-β-Catenin (Cell Signaling, D10A8), anti-Zeb-1 (Cell Signaling, D80D3), anti-Zo-1 (Cell Signaling, D7D12), anti-β-Actin (Cell Signaling, MAB374), and anti-phospho-STAT1 (BD Bioscience, 612233) antibodies.
Davra V., Kumar S., Geng K., Calianese D., Mehta D., Gadiyar V., Kasikara C., Lahey K.C., Chang Y.J., Wichroski M., Gao C., De Lorenzo M.S., Kotenko S.V., Bergsbaken T., Mishra P.K., Gause W.C., Quigley M., Spires T.E, & Birge R.B. (2020). Axl and Mertk receptors cooperate to promote breast cancer progression by combined oncogenic signaling and evasion of host anti-tumor immunity. Cancer research, 81(3), 698-712.
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