We sourced DHP (Batch # B-DHP109, with expiry in May 2022) from the manufacturer, Divya Pharmacy, Haridwar, India. Lipopolysaccharides from Escherichia coli O111:B4 (L2630) and dexamethasone (PHR1526) were procured from Sigma-Aldrich, Bangalore. Standards for HPTLC, UHPLC and GC-MS were purchased from Natural Remedies Private Ltd., Bangalore; Sigma Aldrich, Bangalore; Cayman chemicals, USA; SISCO Research Lab (SRL), India. HPLC-grade chemicals were majorly procured from Merck and Sigma Aldrich.
Hplc grade chemicals
Manufactured by Merck Group
Sourced in United States
HPLC grade chemicals are high-purity solvents and reagents designed for use in high-performance liquid chromatography (HPLC) applications. These chemicals meet strict specifications for low levels of impurities, consistent quality, and reliable performance in HPLC instrumentation. They are formulated to provide accurate and reproducible results in HPLC analysis.
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7 protocols using hplc grade chemicals
1
Phytochemical Profiling of Divya Herbal Powder
2
Anti-malarial Evaluation of Nagbal and Yangchira
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Aristolochia griffithii Hook, commonly called Nagbal, was collected from Kalamati foot Hills of Sonitpur district of Assam, whereas Thalictrum foliolosum DC, commonly called Yangchira, was collected from the hills of Bomdila, West Kameng district of Arunachal Pradesh (Table 1 ). The aqueous extracts of root portion of both the plants has been used in the traditional treat of malaria for many years. Therefore, roots were used as testing material in the present study. The information about the use of plants in malaria treatment was gathered from locals through group discussions. The plants used in the study were identified by Dr. Ashish Kar of Tata Energy Research Institute, Guwahati, India and the voucher specimens of the plant material have been preserved in Defence Research Laboratory, Tezpur, India. Chloroform, ethyl acetate and n-butanol extracts were prepared using HPLC grade chemicals obtained from Merck, India Ltd., whereas water extracts were prepared using Millipore (Merck Millipore, USA) filtered water.
Details of plants used in anti-malarial evaluation against P. falciparum
| Name | Local name | Part used | Collection time | Location (district) | GPS coordinates |
|---|---|---|---|---|---|
| Aristolochia griffithii, Hook | Nagbal | Root | Nov–Dec 2009 | Kalamati foot hills (Sonitpur) | N 26°51′06″ |
| Thalictrum foliolosum, DC | Yangchira | Root | Oct–Dec 2009 | Bomdila (West Kameng) | N 27°19′11″ |
3
Comparative Analysis of Cow and Buffalo Milk
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Cow and buffalo milk was obtained from a farm. For analytical work, HPLC grade chemicals were used and procured from Sigma Chemical Co. (St. Louis, MO, USA). Yoghurt starter culture was purchased from CHR-Hansen, Denmark.
4
Cheese Starter Culture and Lipid Extraction
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Cheese starter culture, L. lactis ssp. lactis and L. lactis ssp. cremoris (DVS-RST-900), and Lactiplantibacillus plantarum “(Lp. plantarum)” (UALp-05) were purchased from Christian Hansen (Horsholm, Denmark). For analytical work, HPLC-grade chemicals were used (Sigma Aldrich, St. Louis, MO, USA). Safflower oil was obtained by mechanical expression of ‘20–25 °C; cold-pressed safflower oil was used for this study.
5
Cheddar Cheese Preparation Protocol
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Cheddar cheese was prepared using the facilities of Acchha Foods, Lahore. Vitamin E (Catalogue No. 10191-41-0) and selenomethionine (Catalogue No. 3211-76-5) were purchased from Sigma Aldrich (St. Louis MO, USA). Rennet and calcium chloride were purchased from Christian Hansen, Denmark. HPLC grade chemicals, were obtained from Sigma Aldrich, USA.
6
HPLC Analysis using Sigma Aldrich Chemicals
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HPLC grade chemicals from Sigma Aldrich were used for HPLC analysis, and analytical grade chemicals from Sigma Aldrich were used for all the analytical procedures. Mili-Q water obtained from Smart2Pure Water Purification System was used for HPLC analysis, and de-aerated distilled water was used for pharmacopoeial buffer preparation.
7
Mycotoxins Extraction and Analysis
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Citrate-(pH 3), acetate-(pH 5) and phosphate-(pH 7) buffers were prepared in the laboratory as described by Faucet-Marquis et al. (2014) . Aflatoxins (AFB1, AFB2, AFG1, AFG2), ochratoxin A (OTA) standards solutions were purchased from Romer Labs (DE, USA). AFM1 (powder) and standards for calibration curve were obtained from Trilogy lab, USA. HPLC grade chemicals used for extraction and analysis of mycotoxins were obtained from Sigma Aldrich, USA. RIDASCREEN R ⃝ Aflatoxins total ELISA kits were purchased from R-Biopharm, Darmstadt,-Germany.
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