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Nuclease free water and microtubes

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Nuclease-free water and microtubes are laboratory equipment designed for use in molecular biology applications. Nuclease-free water is purified water that is free from nucleases, enzymes that can degrade nucleic acids. Microtubes are small, plastic tubes used for storing and handling small volumes of liquids or samples in a laboratory setting.

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Lab products found in correlation

Paraformaldehyde solution, by Merck Group (2 mentions) Mouse anti human epcam mab, by R&D Systems (2 mentions) Mouse monoclonal anti fc blocker igg, by R&D Systems (2 mentions) Ck8 19 efluor 615, by Thermo Fisher Scientific (2 mentions) Anti pan ck ae1 ae3 efluor 615 mab, by Thermo Fisher Scientific (2 mentions) Anti human epcam efluor 660 mab, by Thermo Fisher Scientific (2 mentions) Anti human vimentin alexa fluor 488 mab, by R&D Systems (2 mentions) Anti human vimentin apc mab, by R&D Systems (2 mentions) Triton x 100, by Merck Group (1 mentions) Paraformaldehyde pfa solution, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions) Isopropyl alcohol, by Merck Group (1 mentions) Sodium carbonate and bicarbonate, by Thermo Fisher Scientific (1 mentions) Micro 90, by Thermo Fisher Scientific (1 mentions) Toluidine blue o, by Carolina Biological Supply (1 mentions) Frame sealed incubation chambers, by Bio-Rad (1 mentions) 2 4 morpholino ethane sulfonic acid, by Merck Group (1 mentions)

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Nuclease-free water (659 citations)

3 protocols using nuclease free water and microtubes

1

Comprehensive Immunophenotyping of Tumor Samples

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COC (6013S-04) was purchased from TOPAS Advanced Polymers (Florence, KY). Chemicals and reagents used in these studies included Micro-90, reagent-grade isopropyl alcohol (IPA), phosphate-buffered saline pH = 7.4 (PBS), 2-(4-morpholino)-ethane sulfonic acid (MES), 7.5% bovine serum albumin (BSA), Triton X-100, paraformaldehyde solution (Sigma-Aldrich, St. Louis, MO), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS) (Pierce, Rockford, IL), mouse anti-human EpCAM mAb (R&D Systems, clone#158210, Minneapolis, MN), mouse anti-human Fibroblast Activation Protein α (FAPα) mAb (R&D Systems, clone#427819), mouse monoclonal anti-Fc blocker IgG (R&D Systems,), DAPI, anti-CD45-FITC mAb (eBioscience, clone HI30), anti-CK 8 and 19 mAb (CK8/19-eFluor®615, clone#LP3K, BA17), anti-pan-CK-(AE1/AE3) eFluor®615 mAb (eBioscience, San Diego, CA), anti-human EpCAM-eFluor®660 mAb (eBioscience, clone#1B7), anti-human vimentin-Alexa Fluor®488 mAb (clone 280618 (R&D), and anti-human vimentin-APC mAb (R&D Systems, clone#280618). Nuclease-free water and microtubes (Ambion, Foster City, CA) were used for preparation and storage of all samples and reagents.
Witek M.A., Aufforth R.D., Wang H., Kamande J.W., Jackson J.M., Pullagurla S.R., Hupert M.L., Usary J., Wysham W.Z., Hilliard D., Montgomery S., Bae-Jump V., Carey L.A., Gehrig P.A., Milowsky M.I., Perou C.M., Soper J.T., Whang Y.E., Yeh J.J., Martin G, & Soper S.A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24.
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2

Immunofluorescence Staining Protocol for CTC Characterization

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COC (6013S-04) was purchased from TOPAS Advanced Polymers (Florence, KY). Chemicals and reagents used in these studies included Micro-90, reagent-grade isopropyl alcohol (IPA), phosphate-buffered saline pH = 7.4 (PBS), 2-(4-morpholino)-ethane sulfonic acid (MES), 7.5% bovine serum albumin (BSA), Triton X-100, paraformaldehyde solution (Sigma-Aldrich, St. Louis, MO), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS) (Pierce, Rockford, IL), mouse anti-human EpCAM mAb (R&D Systems, clone#158210, Minneapolis, MN), mouse anti-human Fibroblast Activation Protein α (FAPα) mAb (R&D Systems, clone#427819), mouse monoclonal anti-Fc blocker IgG (R&D Systems,), DAPI, anti-CD45-FITC mAb (eBioscience, clone HI30), anti-CK 8 and 19 mAb (CK8/19-eFluor®615, clone#LP3K, BA17), anti-pan-CK-(AE1/AE3) eFluor®615 mAb (eBioscience, San Diego, CA), anti-human EpCAM-eFluor®660 mAb (eBioscience, clone#1B7), anti-human vimentin-Alexa Fluor®488 mAb (clone 280618 (R&D), and anti-human vimentin-APC mAb (R&D Systems, clone#280618). Nuclease-free water and microtubes (Ambion, Foster City, CA) were used for preparation and storage of all samples and reagents.
Witek M.A., Aufforth R.D., Wang H., Kamande J.W., Jackson J.M., Pullagurla S.R., Hupert M.L., Usary J., Wysham W.Z., Hilliard D., Montgomery S., Bae-Jump V., Carey L.A., Gehrig P.A., Milowsky M.I., Perou C.M., Soper J.T., Whang Y.E., Yeh J.J., Martin G, & Soper S.A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24.
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3

Immobilization of Oligonucleotides on Polymer Surfaces

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PMMA and 6013S-04 COC were purchased from Plaskolite (Columbus, OH) and TOPAS Advanced Polymers (Florence, KY), respectively. Frame-sealed incubation chambers were purchased from Bio-Rad (Hercules, CA). Chemicals and reagents used in these studies included: Micro-90, sodium dodecyl sulfate (SDS), sodium hydroxide, sodium carbonate and bicarbonate (Fisher Scientific, Houston, TX); reagent-grade isopropyl alcohol (IPA), 2-(4-morpholino)-ethane sulfonic acid (MES), bovine serum albumin (BSA), Triton X-100, and paraformaldehyde solution (PFA) (Sigma-Aldrich, St. Louis, MO); toluidine blue O (TBO) (Carolina Biological Supply, Burlington, NC); phosphate-buffered saline (PBS, pH = 7.4) (Life Technologies, Carlsbad, CA); 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) (Pierce, Rockford, IL); Cy3-labeled oligonucleotides (5′NH2-C6-TTT-TTT-TTT-TTC-CGA-CAC-TTA-CGT-TTT-TTT-T-Cy3-3′; Integrated DNA Tech., Coralville, IA); NorthernLights 493-streptavidin (NL493-streptavidin), monoclonal anti-EpCAM Abs, and Fc blocker IgG (R&D Systems, Minneapolis, MN); and 4′,6-diamidino-2-phenylindole (DAPI), and CD45-FITC and cytokeratin 8 and 19 (CK8/19-PK) Abs (eBioscience, San Diego, CA). Nuclease-free water and microtubes (Ambion, Foster City, CA) were used for preparation and storage of all samples and reagents unless noted otherwise.
Jackson J.M., Witek M.A., Hupert M.L., Brady C., Pullagurla S., Kamande J., Aufforth R.D., Tignanelli C.J., Torphy R.J., Yeh J.J, & Soper S.A. (2014). UV activation of polymeric high aspect ratio microstructures: ramifications in antibody surface loading for circulating tumor cell selection. Lab on a chip, 14(1), 106-117.
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