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Aperio eslidemanager database

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Aperio eSlideManager is a digital pathology database solution that provides secure storage and management of whole slide images. The system facilitates the organization, retrieval, and sharing of digital pathology data.

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Lab products found in correlation

Aperio scanscope xt, by Leica (3 mentions) Cytoseal 60, by Thermo Fisher Scientific (3 mentions) Aperio at2, by Leica (3 mentions) Aperio scanscope cs, by Leica (2 mentions) Aperio scanscope fl, by Leica (2 mentions) Immpress hrp anti rabbit igg, by PerkinElmer (2 mentions) Tsa cy3, by PerkinElmer (2 mentions) Prolong gold, by Thermo Fisher Scientific (2 mentions) Tsa cy5, by PerkinElmer (1 mentions) Tmalab, by Leica (1 mentions) Cytoplasmic v2 algorithm, by Leica (1 mentions) Bond 3 autostainer, by Leica (1 mentions)

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ESlideManager database (3 citations)

6 protocols using aperio eslidemanager database

1

Digital Imaging of Stained Slides

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DAB stained slides for SYNGR3 were digitally scanned using the Aperio ScanScope-XT (serial number ss1475, Aperio Technologies; Vista, CA). DAB stained slides for p16 were digitally scanned using Aperio ScanScope CS (serial number ss5072, Leica Biosystems; Buffalo Grove, IL). Multiplex immunofluorescent slides were scanned using the Aperio ScanScope FL (serial number ss6132, Leica Biosystems; Buffalo Grove, IL). All images were scanned at an apparent 20x magnification and uploaded to the Aperio eSlideManager database (version 12.4.3, Leica Biosystems; Buffalo Grove, IL) at the Translational Pathology Laboratory at UNC.
Murphy R.M., Tasoulas J., Porrello A., Carper M.B., Tsai Y.H., Coffey A.R., Kumar S., Zeng P.Y., Schrank T.P., Midkiff B.R., Cohen S., Salazar A.H., Hayward M.C., Hayes D.N., Olshan A., Gupta G.P., Nichols A.C., Yarbrough W.G., Pecot C.V, & Amelio A.L. (2022). Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer. Cancer Research Communications, 2(9), 987-1004.
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2

Multiplex Immunofluorescence Staining Protocol

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Slides were incubated with SYNGR3 Invitrogen antibody at 1:300 for 1hr and was detected with ImmPress HRP anti-rabbit IgG and TSA Cy5 (SAT705A001EA; Perkin Elmer, Waltham, MA). After completion of SYNGR3 staining, a second round of denaturation (10 min, Bond-Epitope Retrieval solution 1) was followed by incubation in either anti- pan-Cytokeratin (30 min, 1:1500) or CD45 (30 min; ready to use) and detection with ImmPress HRP anti-rabbit IgG and TSA Cy3 (SAT704A001EA; Perkin Elmer). Following pan-Cytokeratin/CD45 staining, a third denaturation step was performed for 10 min in Bond-Epitope Retrieval solution 2 (pH 9.0; AR9640) followed by incubation with either anti-CD3 (1hr, 1:200) or p16 (1hr, 1:5) then detection with Bond Polymer (DS9455) and TSA Alexa-488 (B40953, Invitrogen).
Stained slides were dehydrated and coverslipped with either Cytoseal 60 (single DAB stains; 8310–4, Thermo Fisher) or Prolong gold (multiplex stains; P36930, Thermo Fisher). Positive and negative controls (no primary antibody) were included during staining runs. The slides were digitally scanned at 20x magnification using Aperio AT2 (Aperio Technologies, Vista, CA) and uploaded to the Aperio eSlideManager database (Leica Biosystems Inc) at the Pathology Services Core at UNC
Murphy R.M., Tasoulas J., Porrello A., Carper M.B., Tsai Y.H., Coffey A.R., Kumar S., Zeng P.Y., Schrank T.P., Midkiff B.R., Cohen S., Salazar A.H., Hayward M.C., Hayes D.N., Olshan A., Gupta G.P., Nichols A.C., Yarbrough W.G., Pecot C.V, & Amelio A.L. (2022). Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer. Cancer Research Communications, 2(9), 987-1004.
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3

Quantifying CRTC2 Subcellular Localization

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Slides DAB stained for CRTC2 were digitally scanned using the Aperio ScanScope-XT (Aperio Technologies; Vista, CA). All images were scanned at an apparent 20x magnification and uploaded to the Aperio eSlideManager database (version 12.4.3, Leica Biosystems; Buffalo Grove, IL) at the Pathology Services Core at UNC. For analysis of CRTC2 subcellular localization, slides stained CRTC2 were digitally segmented into individual cores using TMALab (Aperio Technologies; Vista, CA). Whole tissue section images were digitally annotated using Aperio ImageScope to remove staining artifacts and tissue folds before they were analyzed. The Cytoplasmic v2 algorithm (Aperio Technologies) was used to analyze CRTC2 staining. Using this algorithm, cells were analyzed for DAB signal and the number and percentage of cells with light (1+), medium (2+) and strong (3+) cell staining was determined. The average DAB intensities in cells, cytoplasm and nuclei were also determined.
Carper M.B., Goel S., Zhang A.M., Damrauer J.S., Cohen S., Zimmerman M.P., Gentile G.M., Parag-Sharma K., Murphy R.M., Sato K., Nickel K.P., Kimple R.J., Yarbrough W.G, & Amelio A.L. (2022). Activation of the CREB Coactivator CRTC2 by Aberrant Mitogen Signaling promotes oncogenic functions in HPV16 positive head and neck cancer. Neoplasia (New York, N.Y.), 29, 100799.
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4

Chromogenic IHC for CRTC2 in Tissue

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Chromogenic immunohistochemistry (IHC) was performed at the UNC Pathology Services Core on paraffin-embedded tissues that were sectioned at 5 micrometers. All IHC was carried out in the Bond III Autostainer (Leica Microsystems Inc.). Slides were dewaxed in Bond Dewax solution (AR9222) and hydrated in Bond Wash solution (AR9590). Antigen retrieval was performed for 20 min at 100°C in Bond-Epitope Retrieval solution 1, pH-6.0 (AR9961). For the CRTC2 antibody (Covance, affinity-purified rabbit polyclonal clone #3364), slides were incubated at a dilution of 1:500 for 1hr then ImmPress HRP anti-rabbit IgG secondary (Vector Laboratories, cat. # MP-7451). Stained slides were dehydrated and coverslipped with Cytoseal 60 (Thermo Fisher, cat. #8310-4). Positive and negative controls (no primary antibody) were included during staining runs. The slides were digitally scanned at 20X magnification using Aperio AT2 (Aperio Technologies, Vista, CA) and uploaded to the Aperio eSlideManager database (Leica Biosystems Inc) at the Pathology Services Core at UNC.
Carper M.B., Goel S., Zhang A.M., Damrauer J.S., Cohen S., Zimmerman M.P., Gentile G.M., Parag-Sharma K., Murphy R.M., Sato K., Nickel K.P., Kimple R.J., Yarbrough W.G, & Amelio A.L. (2022). Activation of the CREB Coactivator CRTC2 by Aberrant Mitogen Signaling promotes oncogenic functions in HPV16 positive head and neck cancer. Neoplasia (New York, N.Y.), 29, 100799.
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5

Digital Scanning of Immunostained Slides

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DAB-stained slides for SYNGR3 were digitally scanned using the Aperio ScanScope-XT (serial number ss1475, Aperio Technologies). DAB-stained slides for p16 were digitally scanned using Aperio ScanScope CS (serial number ss5072, Leica Biosystems). Multiplex immunofluorescent slides were scanned using the Aperio ScanScope FL (serial number ss6132, Leica Biosystems). All images were scanned at an apparent 20× magnification and uploaded to the Aperio eSlideManager database (version 12.4.3, Leica Biosystems) at the Translational Pathology Laboratory at UNC.
Murphy R.M., Tasoulas J., Porrello A., Carper M.B., Tsai Y.H., Coffey A.R., Kumar S., Zeng P.Y., Schrank T.P., Midkiff B.R., Cohen S., Salazar A.H., Hayward M.C., Hayes D.N., Olshan A., Gupta G.P., Nichols A.C., Yarbrough W.G., Pecot C.V, & Amelio A.L. (2022). Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer. Cancer Research Communications, 2(9), 987-1004.
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6

Multiplex Immunofluorescence Staining of Tissue Slides

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Slides were incubated with SYNGR3 Invitrogen antibody at 1:300 for 1 hour and were detected with ImmPress HRP anti-rabbit IgG and TSA Cy5 (SAT705A001EA). After completion of SYNGR3 staining, a second round of denaturation (10 minutes, Bond-Epitope Retrieval solution 1) was followed by incubation in either anti- pan-Cytokeratin (30 minutes, 1:1,500) or CD45 (30 minutes; ready to use) and detection with ImmPress HRP anti-rabbit IgG and TSA Cy3 (SAT704A001EA; Perkin Elmer). Following pan-Cytokeratin/CD45 staining, a third denaturation step was performed for 10 minutes in Bond-Epitope Retrieval solution 2 (pH 9.0; AR9640) followed by incubation with either anti-CD3 (1 hour, 1:200) or p16 (1 hour, 1:5) then detection with Bond Polymer (DS9455) and TSA Alexa-488 (B40953, Invitrogen).
Stained slides were dehydrated and coverslipped with either Cytoseal 60 (single DAB stains; 8310-4, Thermo Fisher Scientific) or Prolong gold (multiplex stains; P36930, Thermo Fisher Scientific). Positive and negative controls (no primary antibody) were included during staining runs. The slides were digitally scanned at 20× magnification using Aperio AT2 (Aperio Technologies) and uploaded to the Aperio eSlideManager database (Leica Biosystems Inc) at the Pathology Services Core at UNC.
Murphy R.M., Tasoulas J., Porrello A., Carper M.B., Tsai Y.H., Coffey A.R., Kumar S., Zeng P.Y., Schrank T.P., Midkiff B.R., Cohen S., Salazar A.H., Hayward M.C., Hayes D.N., Olshan A., Gupta G.P., Nichols A.C., Yarbrough W.G., Pecot C.V, & Amelio A.L. (2022). Tumor Cell Extrinsic Synaptogyrin 3 Expression as a Diagnostic and Prognostic Biomarker in Head and Neck Cancer. Cancer Research Communications, 2(9), 987-1004.
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