Dulbecco s modified eagle medium dmem with glutamax

All the solvents, reagents, and standards used were of analytical grade. The following substances were used in the study: Ethanol 96% (v/v) (AB Strumbras, Kaunas, Lithuania), procyanidin C1, procyanidin B2, quercetin, hyperoside, avicularin, quercitrin, kaempherol 3-O-glucoside, luteolin 7-O-glucoside, phloridzin, formic acid, acetonitrile, (+)-catechin, (-)-epicatechin, rutin, isoquercitrin, chlorogenic acid, p-coumaric acid, caffeic acid, hydrochloric acid, Hoechst33342, propidium iodide, glucose, temozolomide, DMSO and KCl (Sigma-Aldrich, Steinheim, Germany), Dulbecco’s Modified Eagle Medium (DMEM) with Glutamax, foetal bovine serum, penicillin-streptomycin, Versene solution, antibiotic-antimycotic solution (Anti-Anti) were of Gibco brand and purchased from Thermo Fisher Scientific, Waltham, MA, USA. During the study, we used purified de-ionized water prepared with the Milli–Q^® (Millipore, Bedford, MA, USA) water purification system.

The Plant Genomic DNA Extract Mini Kit was purchased from Fisher Molecular Biology (Rome, Italy). The Qubit dsDNA HS Assay Kit was purchased from Invitrogen (Carlsbad, CA, USA). The PCR Mix Plus was purchased from A&A Biotechnology (Gdansk, Poland). HepG2 cell line (ATCC^® HB-8065™) was purchased from ATCC (Manassas, VA, USA). Dulbecco’s modified Eagle medium (DMEM) with GlutaMAX was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Phosphate-buffered saline (PBS), penicillin–streptomycin mix, dimethyl sulfoxide (DMSO), and Cholesterol Quantitation Kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Euroclone (Milan, Italy). CellTiter 96^® AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega (Madison, WI, USA). The Total Bile Acid Assay Kit (Fluorometric) was purchased from Cell Biolabs San Diego, CA, USA). OriginLab software was purchase from OriginLab Corporation (Northampton, MA, USA).