The monocytic cell line THP-1 and alveolar epithelium cell line A549 were used to quantify the toxicity of the irradiation on human cells. Both cell lines were incubated at 37 °C in a 5% CO2 atmosphere in ambient humidified air. THP-1 cells were grown in RPMI 1640 (Gibco™ RPMI 1640 Medium, GlutaMAX™, Life Technologies Limited, Paisley, UK) containing phenol red, supplemented with 0.01 M HEPES Buffer (PAN-Biotech, Aidenbach, Germany), 10% v/v fetal bovine serum (FBS superior stabil, Bio&Sell, Feucht, Germany) and 0.2% v/v 2-mercaptoethanol (SERVA Electrophoresis, Heidelberg, Germany). The A549 cells were incubated in DMEM medium (DMEM, high glucose, Life Technologies Limited, Paisley, UK) containing phenol red, supplemented with 10% v/v fetal bovine serum (FBS superior stabil, Bio&Sell, Feucht, Germany) and 1 mM sodium pyruvate (Life Technologies Limited, Paisley, UK). Both cell culture media were supplemented with 1% v/v Penicillin–Streptomycin (PAN-Biotech, Aidenbach, Germany).
Fbs superior stabil
Manufactured by Bio&Sell
Sourced in Germany
FBS superior stabil is a specialized lab equipment designed for the stabilization of fetal bovine serum (FBS). Its core function is to provide a controlled environment to preserve the integrity and quality of FBS samples.
Automatically generated - may contain errors
Lab products found in correlation
Hepes buffer, by PAN Biotech (1 mentions)
Penicillin streptomycin, by PAN Biotech (1 mentions)
Collagenase a, by Roche (1 mentions)
Collagenase type 4, by Merck Group (1 mentions)
Antibiotic antimycotic solution, by Merck Group (1 mentions)
Schneider s drosophila medium, by Thermo Fisher Scientific (1 mentions)
Mccoy s medium, by Thermo Fisher Scientific (1 mentions)
Vero e6, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
4 protocols using fbs superior stabil
1
Cytotoxicity of Radiation Exposure on Human Cells
2
Isolation and Culture of Tumor Cells
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Tumor pieces were digested either with 2.5 mg/mL Collagenase A (Roche, Mannheim, Germany) or 1 mg/mL Collagenase type IV (Sigma-Aldrich, Munich, Germany). The amount of enzyme solution corresponded to five times the volume of the tumor pieces. The samples were shaken continuously in the water bath at 37°C. Every 30 min, the supernatant was collected, and the digestion was stopped with the same volume of serum-containing medium, while the solid residues were incubated with fresh enzyme solution. After all solid tissue was digested, cells were centrifuged at 200 g for 3 min. Cell pellets were suspended with Dulbecco's modified Eagle's medium (DMEM) (Thermo Fisher Scientific, Waltham, MA), 10% standardized fetal bovine serum (FBS SUPERIOR stabil®) (Bio & Sell GmbH, Feucht, Germany), and 1% antibiotic–antimycotic solution (Sigma-Aldrich). The cells were cultured for 21 days on 6 cm Petri dishes under standard culture conditions in a humidified incubator containing 5% CO2 at 37°C. Cell culture media were exchanged every 2–3 days.
3
Cell Culture Protocols for Drosophila, COS-7, HeLa, and U-2 OS
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Drosophila S2 cells (Cat. No. R69007, Lot No. 2082623; Thermo Fisher Scientific/Gibco) were cultivated in Schneider’s Drosophila medium (Cat. No. 21720024; Merck/Sigma-Aldrich/Gibco) supplemented with 1mM sodium pyruvate (Cat. No. S8636; Merck/Sigma-Aldrich) and 10% (vol/vol) fetal bovine serum (Cat. No. FBS. S 0615, FBS Superior stabil; Bio & Sell GmbH). Cells were cultivated at 28°C and ambient CO2 levels. Kidney fibroblast-like cells (COS-7) from the green African monkey C. aethiops (Cat. No. 87021302, Lot No. 05G008; Merck/Sigma-Aldrich) and human cervical cancer cells (HeLa) were cultivated in DMEM, containing 4.5 g/liter glucose and GlutaMAX additive (Cat. No. 10566016; Thermo Fisher Scientific) supplemented with 1 mM sodium pyruvate (Cat. No. S8636; Merck/Sigma-Aldrich) and 10% (vol/vol) FBS at 37°C and 5% CO2. Human osteosarcoma cells (U-2 OS) (Cat. No. 92022711, ECACC) were cultivated at 37°C and 5% CO2 and grown in McCoy’s medium (Thermo Fisher Scientific) supplemented with 10% (vol/vol) FBS and 1% (vol/vol) sodium pyruvate.
4
Cell Culture Protocols for Virus Research
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Vero E6 (Cercopithecus aethiops derived epithelial kidney) cells were purchased from ATCC® and grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) which was supplemented with 2.5% heat-inactivated fetal calf serum (FCS), 100 units/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1x non-essential amino acids. ELVIS cells (Enzyme-Linked Virus-Inducible System—ELVIS™), also from ATCC® are genetically engineered baby hamster kidney cells that encode a lacZ gene, which is expressed upon infection via the viral transactivator ICP10 (Proffitt and Schindler, 1995 (link)). TZM-bl cells are HeLa derived cell line expressing CD4, CCR5 and CXCR4, encoding luciferase and β-galactosidase genes under the control of the HIV-LTR promoter (Wei et al., 2002 (link)). ELVIS and TZM-bl cells were grown in DMEM supplemented with 2 mM L-glutamine, 100 units/ml penicillin, and 100 μg/ml streptomycin and 10% heat-inactivated FCS. Monocytic THP-1 cells were cultivated in Roswell Park Memorial Institute 1640 medium (RPMI 1640, Gibco™ RPMI 1640 Medium, GlutaMAX™, Life Technologies Limited) supplemented with 0.01 M HEPES Buffer (PAN-Biotech, Aidenbach, Germany), 10% v/v fetal bovine serum (FBS superior stabil, Bio&Sell GmbH, Feucht, Germany) and 0.2% v/v 2-mercaptoethanol (SERVA Electrophoresis GmbH, Heidelberg, Germany). Cells were incubated at 37°C and 5% CO2.
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