HLE, MHCC97H, SNU449, and Huh7 human HCC cell lines, and KKU156, KKU213, and RBE iCCA cell lines were used for the in vitro studies. The HLE, KKU156, and KKU213 cell lines were purchased from the JCRB Cell Bank, whereas SNU449, Huh7, and RBE cell lines were purchased from ATCC. The MHCC97H cell line was a kind gift from Dr. Binbin Liu from Fudan University, Shanghai, China. Cell lines were validated (Genetica DNA Laboratories, Burlington, NC, USA) and maintained as monolayer cultures in Dulbecco’s modified Eagle medium with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 100 U/mL penicillin, and 100 g/mL streptomycin (Gibco). Transfection with lentivirus and colony formation assay were performed as described before in detail (23 (link)).
Kku156
Manufactured by Japanese Collection of Research Bioresources Cell Bank
KKU156 is a laboratory equipment used for cell culture applications. It serves as an incubator that provides a controlled environment for the growth and maintenance of cells. The core function of KKU156 is to regulate temperature, humidity, and CO2 levels to support optimal conditions for cell culture.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Snu 449, by American Type Culture Collection (1 mentions)
Kku 214, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hucca 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku 055, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku 100, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Tfk 1, by RIKEN Cell Bank (1 mentions)
Ssp 25, by RIKEN Cell Bank (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Mmnk 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku 213, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hucct1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
2 protocols using kku156
1
Comprehensive Cell Line Analysis for HCC and iCCA
2
Bile Duct Cancer Cell Migration Assay
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KKU-213, KKU-214, KKU-156, KKU-100, KKU-055, HuCCA-1, HuCCT1 and MMNK-1 were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank, Japan. RBE, SSP-25, and TFK-1 were obtained from RIKEN Cell bank, Japan. KKK-D068, KKK-D131, KKK-D138 were obtained from Khon Kaen University, Thailand18 (link). MCF7, HCT116, HeLa, A549 were purchased from ATCC. All cells were maintained at 37 °C in a humidified 5% CO2 atmosphere using growth media that were recommended by original cell sources (see complete details in Supplementary Table S1 ). The cell line panel consists of cancer of bile duct, which is known to be relatively migrative. Other cell lines with similar migratory property also include breast cancer (MCF7), lung cancer (A549), colorectal cancer (HCT116), and cervical cancer (HeLa). However, to test our novel method we selected a set of bile duct carcinoma cell lines known to be migrative as our model.
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