Endoglycosidase hf endo hf

Manufactured by New England Biolabs

Endoglycosidase Hf (Endo Hf) is an enzyme that cleaves the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins. It is used to remove N-linked oligosaccharides from glycoproteins.

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Lab products found in correlation

Chymotrypsin, by Promega (2 mentions) Fetuin, by New England Biolabs (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) O glycosidase, by New England Biolabs (2 mentions) Sequencing grade trypsin, by Promega (2 mentions) Neuraminidase, by New England Biolabs (2 mentions) Direct q3, by Merck Group (2 mentions) Glycerol free peptidyl n glycosidase f pngase f, by New England Biolabs (2 mentions) 4 vinylpyridine, by Merck Group (2 mentions) Trizma hydrochloride, by Merck Group (2 mentions) Trizma base, by Merck Group (2 mentions) Ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Optima lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Ethanol, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Urea, by Merck Group (1 mentions) Ammonium bicarbonate, by Merck Group (1 mentions)

Close spelling variants of this product

Endoglycosidase Hf (9 citations) Endo Hf (83 citations)

2 protocols using endoglycosidase hf endo hf

Monoclonal Antibody Characterization Protocol

Cited 3 times

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Trizma hydrochloride, Trizma base, ammonium bicarbonate, urea, Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), iodoacetamide (IAM), ethanol, 4-vinylpyridine (4-VP), and glacial acetic acid were purchased from Sigma. Other reagents used in this study included optima liquid chromatography (LC)-MS-grade acetonitrile, water, formic acid (Fisher Scientific), sequencing-grade trypsin (Promega), chymotrypsin (Promega), glycerol-free peptidyl-N-glycosidase F (PNGase F) (New England Biolabs), endoglycosidase Hf (Endo Hf) (New England Biolabs), O-glycosidase (New England Biolabs), neuraminidase (New England Biolabs), and fetuin (New England Biolabs). All reagents and buffers were prepared with deionized water purified with a Millipore Direct-Q3 (Billerica, MA) water purification system.
The 2–4, 4–8, and 2–43 monoclonal antibodies (MAbs) were a kind gift from the laboratory of David D. Ho (Columbia University Vagelos College of Physicians and Surgeons) (25 (link), 80 (link), 81 (link)). The CR3022 MAb was purchased from Abcam (12 (link), 82 (link), 83 (link)).

Zhang S., Go E.P., Ding H., Anang S., Kappes J.C., Desaire H, & Sodroski J.G. (2022). Analysis of Glycosylation and Disulfide Bonding of Wild-Type SARS-CoV-2 Spike Glycoprotein. Journal of Virology, 96(3), e01626-21.

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Glycoprotein Analysis Using Biochemical Techniques

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Trizma^© hydrochloride, Trizma^© base, ammonium bicarbonate, urea, tris(2-carboxyethyl) phosphine hydrochloride (TCEP), iodoacetamide (IAM), ethanol, 4-vinylpyridine (4-VP), and glacial acetic acid were purchased from Sigma. Other reagents used in this study included optima LC/MS grade acetonitrile, water, formic acid (Fisher Scientific), sequencing grade trypsin (Promega), chymotrypsin (Promega), glycerol-free peptidyl-N-glycosidase F (PNGase F) (New England Biolabs), Endoglycosidase Hf (Endo Hf) (New England Biolabs), O-glycosidase (New England Biolabs), neuraminidase (New England Biolabs) and fetuin (New England Biolabs). All reagents and buffers were prepared with deionized water purified with a Millipore Direct-Q3 (Billerica, MA) water purification system.

Zhang S., Go E.P., Ding H., Anang S., Kappes J.C., Desaire H, & Sodroski J. (2021). Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein. bioRxiv.

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