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Citraconic anhydride for

Manufactured by Thermo Fisher Scientific
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Citraconic anhydride is a chemical compound used as a starting material in the synthesis of various organic compounds. It serves as a building block for the production of other chemicals and materials in laboratory settings.

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2 protocols using citraconic anhydride for

1

Fibroblast Proteomics and Metabolism

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High-performance liquid chromatography-grade acetonitrile, ethanol, methanol, water, trifluoroacetic acid (TFA), bleomycin (B5507, Lot#SLCG3757), and recombinant isoamylase were purchased from Sigma-Aldrich. α-cyano-4-hydroxycinnamic acid (CHCA) matrix was purchased from Cayman Chemical. Histological-grade xylenes were purchased from Spectrum Chemical. Citraconic anhydride for antigen retrieval was obtained from Thermo Fisher Scientific. Recombinant PNGaseF Prime was obtained from N-Zyme Scientifics (Doylestown, PA, USA). Normal lung fibroblast isolated from adult lung tissue and diseased lung fibroblast isolated from adult idiopathic pulmonary fibrosis lung tissue were purchased from Lonza, USA (Cat# CC-2512 and CC-7231, respectively). 13C6-glucose was purchased from Cambridge Isotope Laboratories, Inc (Cat# CLM-1396-pk).
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2

MALDI-TOF Mass Spectrometry Protocol

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Trifluoroacetic acid and alpha-cyano-4-hydroxycinnamic acid (CHCA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade methanol, ethanol, acetonitrile, xylene, and water were obtained from Fisher Scientific (Pittsburgh, PA, USA). Tissue Tack positively charged microscope slides were purchased from Polysciences, Inc. (Warrington, PA, USA). Citraconic anhydride for antigen retrieval was from Thermo Scientific (Bellefonte, PA, USA). Recombinant PNGaseF was obtained from the laboratory of Dr. Anand Mehta (Charleston, SC, USA), as described.[14 (link)] Hematoxylin and eosin (H&E) stains were obtained from Cancer Diagnostics (Durham, NC, USA).
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