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Alexa fluor 594 donkey anti mouse igg1

Manufactured by Thermo Fisher Scientific
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Sourced in United States

Alexa Fluor 594 donkey anti-mouse IgG1 is a secondary antibody conjugated with the Alexa Fluor 594 fluorescent dye. It is designed to detect and visualize mouse IgG1 primary antibodies in various immunoassays and imaging applications.

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Lab products found in correlation

Alexa fluor 488 goat anti mouse igg2b, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Confocal laser scanning microscope, by Olympus (2 mentions)

Close spelling variants of this product

Anti-mouse IgG1 Alexa-Fluor 594 Alexa Fluor 594 donkey anti-mouse Donkey anti-mouse Alexa 594 Alexa Fluor 594 anti-mouse Alexa Fluor donkey anti-mouse Alexa Fluor 594 Anti-mouse Alexa Fluor Alexa 594 Mouse IgG1 Alexa Fluors

2 protocols using alexa fluor 594 donkey anti mouse igg1

1

Immunofluorescence Analysis of Cellular Proteins

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For immunofluorescence analysis, cells were incubated first with primary antibodies and then with Alexa Fluor 594 donkey anti-mouse IgG1 (Life Technologies, Carlsbad, CA, USA) and Alexa Fluor 488 goat anti-mouse IgG2b (Life Technologies) secondary antibodies. Cells were washed 3 times in PBS, after which nuclei were counterstained with 4’,6-diamidino-2-phenylindole (Life Technologies). Images were obtained on a confocal laser scanning microscope (Olympus, Tokyo, Japan).
Li Y., Xie K., Wang C., Yang C., Huang K., Li F., Zheng C., Chen J., Dong S., Deng G., Huang G., Lu Q., Liu J., Li K., Tang Y, & Wang L. (2021). 3D Printing of Tricalcium Phosphate/Poly Lactic-co-glycolic Acid Scaffolds Loaded with Carfilzomib for Treating Critical-sized Rabbit Radial Bone Defects. International Journal of Bioprinting, 7(4), 405.
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2

Immunofluorescence Imaging of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence analysis, after incubation with primary antibodies, followed by Alexa Fluor 594 donkey anti-mouse IgG1 (Life Technologies, Carlsbad, CA, USA) and Alexa Fluor 488 goat anti-mouse IgG2b (Life Technologies) secondary antibodies, cells were washed 3 times in PBS, after which nuclei were counterstained with 4’,6-diamidino-2-phenylindole (Life Technologies). Images were obtained on a confocal laser-scanning microscope (Olympus, Tokyo, Japan).
Zheng C., Attarilar S., Li K., Wang C., Liu J., Wang L., Yang J, & Tang Y. (2021). 3D-printed HA15-loaded β-Tricalcium Phosphate/Poly (Lactic-co-glycolic acid) Bone Tissue Scaffold Promotes Bone Regeneration in Rabbit Radial Defects. International Journal of Bioprinting, 7(1), 317.
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